Metalloprotein design using genetic code expansion

Hu, C., Chan, S.I., Sawyer, E.B., Yu, Y. and Wang, J. 2014. Metalloprotein design using genetic code expansion. Chemical Society Reviews. 43, pp. 6498-6510. https://doi.org/10.1039/c4cs00018h

TitleMetalloprotein design using genetic code expansion
AuthorsHu, C., Chan, S.I., Sawyer, E.B., Yu, Y. and Wang, J.
Abstract

More than one third of all proteins are metalloproteins. They catalyze important reactions such as photosynthesis, nitrogen fixation and CO2 reduction. Metalloproteins such as the olfactory receptors also serve as highly elaborate sensors. Here we review recent developments in functional metalloprotein design using the genetic code expansion approach. We show that, through the site-specific incorporation of metal-chelating unnatural amino acids (UAAs), proton and electron transfer mediators, and UAAs bearing bioorthogonal reaction groups, small soluble proteins can recapitulate and expand the important functions of complex metalloproteins. Further developments along this route may result in cell factories and live-cell sensors with unprecedented efficiency and selectivity.

JournalChemical Society Reviews
Journal citation43, pp. 6498-6510
ISSN0306-0012
1460-4744
Year2014
PublisherRoyal Society of Chemistry
Digital Object Identifier (DOI)https://doi.org/10.1039/c4cs00018h
Web address (URL)http://www.scopus.com/inward/record.url?eid=2-s2.0-84906282231&partnerID=MN8TOARS
Publication dates
Published2014

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