Self-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering

Sawyer, E.B. and Gras, S.L. 2013. Self-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering. in: Gerrard, J.A. (ed.) Protein Nanotechnology Humana Press.

Chapter titleSelf-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering
AuthorsSawyer, E.B. and Gras, S.L.
EditorsGerrard, J.A.
Abstract

Amyloid fibrils are attractive targets for applications in biotechnology. These thin, nanoscale protein fibers are highly ordered structures that self-assemble from their component proteins or peptides. This chapter describes the use of several biophysical techniques to monitor the formation of amyloid fibrils including a common dye-binding assay, turbidity assay, and small-angle X-ray scattering. These techniques provide information about the assembly mechanism, the rate and reproducibility of assembly, as well as the size of species along the assembly pathway. © 2013 Springer Science+Business Media, New York.

Book titleProtein Nanotechnology
Year2013
PublisherHumana Press
Publication dates
Published2013
SeriesMethods in Molecular Biology
ISBN9781627033534
ISSN1064-3745
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-62703-354-1_5
PubMed ID23504419
Web address (URL)http://www.scopus.com/inward/record.url?eid=2-s2.0-84877148212&partnerID=MN8TOARS
JournalMethods in Molecular Biology

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