Sawyer, E.B. and Gras, S.L. 2013. Self-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering. in: Gerrard, J.A. (ed.) Protein Nanotechnology Humana Press.
| Chapter title | Self-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering |
|---|---|
| Authors | Sawyer, E.B. and Gras, S.L. |
| Editors | Gerrard, J.A. |
| Abstract | Amyloid fibrils are attractive targets for applications in biotechnology. These thin, nanoscale protein fibers are highly ordered structures that self-assemble from their component proteins or peptides. This chapter describes the use of several biophysical techniques to monitor the formation of amyloid fibrils including a common dye-binding assay, turbidity assay, and small-angle X-ray scattering. These techniques provide information about the assembly mechanism, the rate and reproducibility of assembly, as well as the size of species along the assembly pathway. © 2013 Springer Science+Business Media, New York. |
| Book title | Protein Nanotechnology |
| Year | 2013 |
| Publisher | Humana Press |
| Publication dates | |
| Published | 2013 |
| Series | Methods in Molecular Biology |
| ISBN | 9781627033534 |
| ISSN | 1064-3745 |
| Digital Object Identifier (DOI) | https://doi.org/10.1007/978-1-62703-354-1_5 |
| PubMed ID | 23504419 |
| Web address (URL) | http://www.scopus.com/inward/record.url?eid=2-s2.0-84877148212&partnerID=MN8TOARS |
| Journal | Methods in Molecular Biology |