• Journal article

Combining experiment and energy landscapes to explore anaerobic heme breakdown in multifunctional hemoproteins

Alasdair D. Keith, Elizabeth B. Sawyer, Desmond Choy, Yuhang Xie, George Biggs, Oskar James Klein, Paul D. Brear, David Wales and Paul Barker 2024. Combining experiment and energy landscapes to explore anaerobic heme breakdown in multifunctional hemoproteins. Physical Chemistry Chemical Physics. 26, pp. 695-712. https://doi.org/10.1039/d3cp03897a

TitleCombining experiment and energy landscapes to explore anaerobic heme breakdown in multifunctional hemoproteins
TypeJournal article
AuthorsAlasdair D. Keith, Elizabeth B. Sawyer, Desmond Choy, Yuhang Xie, George Biggs, Oskar James Klein, Paul D. Brear, David Wales and Paul Barker
Abstract

To survive, many pathogens extract heme from their host organism and break down the porphyrin scaffold to sequester the Fe2+ ion via a heme oxygenase. Recent studies have revealed that certain pathogens can anaerobically degrade heme. Our own research has shown that one such pathway proceeds via NADH-dependent heme degradation, which has been identified in a family of hemoproteins from a range of bacteria. HemS, from Yersinia enterocolitica, is the main focus of this work, along with HmuS (Yersinia pestis), ChuS (Escherichia coli) and ShuS (Shigella dysenteriae). We combine experiments, Energy Landscape Theory, and a bioinformatic investigation to place these homologues within a wider phylogenetic context. A subset of these hemoproteins are known to bind certain DNA promoter regions, suggesting not only that they can catalytically degrade heme, but that they are also involved in transcriptional modulation responding to heme flux. Many of the bacterial species responsible for these hemoproteins (including those that produce HemS, ChuS and ShuS) are known to specifically target oxygen-depleted regions of the gastrointestinal tract. A deeper understanding of anaerobic heme breakdown processes exploited by these pathogens could therefore prove useful in the development of future strategies for disease prevention.

JournalPhysical Chemistry Chemical Physics
Journal citation26, pp. 695-712
ISSN1463-9076
1463-9084
Year2024
PublisherRoyal Society of Chemistry
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1039/d3cp03897a
Web address (URL)http://dx.doi.org/10.1039/d3cp03897a
Publication dates
Published in print2024
Published online29 Nov 2023

Related outputs

Ribosome profiling enhances understanding of mycobacterial translation
Sawyer, E.B. and Cortes, T. 2022. Ribosome profiling enhances understanding of mycobacterial translation. Frontiers in Microbiology. 13 976550. https://doi.org/10.3389/fmicb.2022.976550

A snapshot of translation in Mycobacterium tuberculosis during exponential growth and nutrient starvation revealed by ribosome profiling
Sawyer, E.B., Phelan, J.E., Clark, T.G. and Cortes, T. 2021. A snapshot of translation in Mycobacterium tuberculosis during exponential growth and nutrient starvation revealed by ribosome profiling. Cell Reports. 34 (5) 108695. https://doi.org/10.1016/j.celrep.2021.108695

Structural effects of the highly protective V127 polymorphism on human prion protein.
Hosszu LLP, Conners R, Sangar D, Batchelor M, Sawyer EB, Fisher S, Cliff MJ, Hounslow AM, McAuley K, Leo Brady R, Jackson GS, Bieschke J, Waltho JP and Collinge J 2020. Structural effects of the highly protective V127 polymorphism on human prion protein. Communications Biology. 3 402. https://doi.org/10.1038/s42003-020-01126-6

Translational regulation in mycobacteria and its implications for pathogenicity
Sawyer, E.B., Grabowska, A. and Cortes, T. 2018. Translational regulation in mycobacteria and its implications for pathogenicity. Nucleic Acids Research. 46 (14), pp. 6950-6961. https://doi.org/10.1093/nar/gky574

Preclinical detection of infectivity and disease-specific PrP in blood throughout the incubation period of prion disease
Sawyer, E.B., Edgeworth, J.A., Thomas, C., Collinge, J. and Jackson, G.S. 2015. Preclinical detection of infectivity and disease-specific PrP in blood throughout the incubation period of prion disease. Scientific Reports. 5 17742. https://doi.org/10.1038/srep17742

Self-assembly of amyloid fibrils that display active enzymes
Zhou, X.-M., Entwistle, A., Zhang, H., Jackson, A.P., Mason, T.O., Shimanovich, U., Knowles, T.P.J., Smith, A.T., Sawyer, E.B. and Perrett, S. 2014. Self-assembly of amyloid fibrils that display active enzymes. ChemCatChem. 6 (7), pp. 1961-1968. https://doi.org/10.1002/cctc.201402125

Metalloprotein design using genetic code expansion
Hu, C., Chan, S.I., Sawyer, E.B., Yu, Y. and Wang, J. 2014. Metalloprotein design using genetic code expansion. Chemical Society Reviews. 43, pp. 6498-6510. https://doi.org/10.1039/c4cs00018h

Self-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering
Sawyer, E.B. and Gras, S.L. 2013. Self-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering. in: Gerrard, J.A. (ed.) Protein Nanotechnology Humana Press.

Permalink - https://westminsterresearch.westminster.ac.uk/item/w7qqx/combining-experiment-and-energy-landscapes-to-explore-anaerobic-heme-breakdown-in-multifunctional-hemoproteins


Share this

Tweet
Email

Usage statistics

28 total views
11 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.