Dual-acting therapeutic proteins for intraocular use

University of Westminster HUBS L&T workshop ‘Authentic assessments in an AI world’
Smith, C.L. and Guiliano, D.B. 2024. University of Westminster HUBS L&T workshop ‘Authentic assessments in an AI world’.

Bioengineering of Antibody Fragments: Challenges and Opportunities.
Pirkalkhoran, Sama, Grabowska, Wiktoria Roksana, Kashkoli, Hamid Heidari, Mirhassani, Reihaneh, Guiliano, David, Dolphin, Colin and Khalili, H. 2023. Bioengineering of Antibody Fragments: Challenges and Opportunities. Bioengineering. 10 (2) 122. https://doi.org/10.3390/bioengineering10020122

Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection
Srey, M.T., Taccogna, A., Oksov, Y., Lustigman, S., Tai, P-Y., Acord, J., Selkirk, M.E., Lamb, T.J. and Guiliano, D.B. 2020. Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. PLoS Neglected Tropical Diseases. https://doi.org/10.1371/journal.pntd.0008842

Hybridization to High-Density Filter Arrays of a Brugia malayi BAC Library with Biotinylated Oligonucleotides and PCR Products
Foster, J.M., Kamal, I.H., Daub, J., Swan, M.C., Ingram, J.R., Ganatra, M., Ware, J., Guiliano, D.B., Aboobaker, A., Moran, L., Blaxter, M. and Slatko, B.E. 2018. Hybridization to High-Density Filter Arrays of a Brugia malayi BAC Library with Biotinylated Oligonucleotides and PCR Products. BioTechniques. 30 (6), pp. 1216-1224. https://doi.org/10.2144/01306bm06

Polymorphisms in the F Pocket of HLA-B27 Subtypes Strongly Affect Assembly, Chaperone Interactions, and Heavy-Chain Misfolding
Guiliano, D.B., North, H., Panayoitou, E., Campbell, E.C., McHugh, K., Cooke, F.G.M., Silvestre, M., Bowness, P., Powis, S.J. and Antoniou, A.N. 2017. Polymorphisms in the F Pocket of HLA-B27 Subtypes Strongly Affect Assembly, Chaperone Interactions, and Heavy-Chain Misfolding. Arthritis & Rheumatology. 69 (3), pp. 610-621. https://doi.org/10.1002/art.39948

Functional Heterologous Protein Expression by Genetically Engineered Probiotic Yeast Saccharomyces boulardii
Hudson, L.E., Fasken, M.B., McDermott, C.D., McBride, S.M., Kuiper, E.G., Guiliano, D.B., Corbett, A.H. and Lamb, T.J. 2014. Functional Heterologous Protein Expression by Genetically Engineered Probiotic Yeast Saccharomyces boulardii. PLoS ONE. 9 (11) e112660. https://doi.org/10.1371/journal.pone.0112660

Endoplasmic Reticulum Degradation-Enhancing α-Mannosidase-like Protein 1 Targets Misfolded HLA-B27 Dimers for Endoplasmic Reticulum-Associated Degradation
Guiliano, D.B., Fussell, H., Lenart, I., Tsao, E., Nesbeth, D., Fletcher, A.J., Campbell, E.C., Yousaf, N., Williams, S., Santos, S., Cameron, A., Towers, G.J., Kellam, P., Hebert, D.N., Gould, K.G., Powis, S.J. and Antoniou, A.N. 2014. Endoplasmic Reticulum Degradation-Enhancing α-Mannosidase-like Protein 1 Targets Misfolded HLA-B27 Dimers for Endoplasmic Reticulum-Associated Degradation. Arthritis & Rheumatology. 66 (11), pp. 2915-3250. https://doi.org/10.1002/art.38809

Measuring Synthesis and Degradation of MHC Class I Molecules
Guiliano, D.B. and Antoniou, A.N. 2012. Measuring Synthesis and Degradation of MHC Class I Molecules. in: van Endert, P. (ed.) Antigen Processing Springer. pp. 93-108

The MHC Class I Heavy Chain Structurally Conserved Cysteines 101 and 164 Participate in HLA-B27 Dimer Formation
Lenart, I., Guiliano, D.B., Burn, G., Campbell, E.C., Morley, K.D., Fussell, H., Powis, S.J. and Antoniou, A.N. 2012. The MHC Class I Heavy Chain Structurally Conserved Cysteines 101 and 164 Participate in HLA-B27 Dimer Formation. Antioxidants and Redox Signaling. 16 (1), pp. 33-43. https://doi.org/10.1089/ars.2010.3693

Antigen Processing and Presentation by MHC Class I, II, and Nonclassical Molecules
Antoniou, A.N., Lenart, I., Guiliano, D.B. and Powis, S.J. 2012. Antigen Processing and Presentation by MHC Class I, II, and Nonclassical Molecules. in: Morrow, W.J.W., Sheikh, N.A., Schmidt, C.S. and Davies, D.H. (ed.) Vaccinology: Principles and Practice Wiley.

Introduction to helminth infections
Guiliano, D. 2012. Introduction to helminth infections. in: Lamb, T.J. (ed.) Immunity to Parasitic Infections Wiley-Blackwell.

Introduction to protozoan infections
Guiliano, D.B. and Lamb, T.J. 2012. Introduction to protozoan infections. in: Lamb, T.J. (ed.) Immunity to Parasitic Infections Wiley-Blackwell.

Pathogenicity of Misfolded and Dimeric HLA-B27 Molecules
Antoniou, A.N., Lenart, I. and Guiliano, D.B. 2011. Pathogenicity of Misfolded and Dimeric HLA-B27 Molecules. International Journal of Rheumatology. 2011 Article ID 486856. https://doi.org/10.1155/2011/486856

The Oxidative Folding and Misfolding of Human Leukocyte Antigen-B27
Antoniou, A.N., Guiliano, D.B., Lenart, I., Burn, G. and Powis, S.J. 2011. The Oxidative Folding and Misfolding of Human Leukocyte Antigen-B27. Antioxidants and Redox Signaling. 15 (2), pp. 669-684. https://doi.org/10.1089/ars.2010.3692

Trichinella and the Nurse Cell
Guiliano, D.B. and Oksov, Y. 2009. Trichinella and the Nurse Cell. in: Schaible, U.E. and Haas, A. (ed.) Intracellular Niches of Microbes: A Pathogens Guide Through the Host Cell Wiley. pp. 669-688

Draft Genome of the Filarial Nematode Parasite Brugia malayi
Ghedin, E., Wang, S., Spiro, D., Caler, E., Zhao, Q., Crabtree, J., Allen, J.E., Delcher, A.L., Guiliano, D.B., Miranda-Saavedra, D., Samuel V. Angiuoli, S.V., Todd Creasy, T., Paolo Amedeo, P., Brian Haas, B., El-Sayed, N.M., Wortman, J.R., Feldblyum, T., Tallon, L., Schatz, M., Shumway, M., Koo, H., Salzberg, S.L., Schobel, S., Pertea, M., Pop, M., White, O., Barton, G.J., Carlow, C.K.S., Crawford, M.J., Daub, J., Dimmic, M.W., Estes, C.F., Foster, J.M., Ganatra, M., Gregory, W.F., Johnson, N.M., Jinming Jin, J., Richard Komuniecki, R., Ian Korf, I., Sanjay Kumar, S., Sandra Laney, S., Ben-Wen Li, B-W., Wen Li, W., Lindblom, T.H., Lustigman, S., Ma, D., Maina, C.V., Martin, D.M.A., McCarter, J.P., McReynolds, L. and Mitreva, M. 2007. Draft Genome of the Filarial Nematode Parasite Brugia malayi. Science. 317 (5845), pp. 1756-1760. https://doi.org/10.1126/science.1145406

Operon Conservation and the Evolution of trans-Splicing in the Phylum Nematoda
Guiliano, D.B. and Blaxter, M.L. 2006. Operon Conservation and the Evolution of trans-Splicing in the Phylum Nematoda. PLoS Genetics. 2 (11) e198. https://doi.org/10.1371/journal.pgen.0020198

Characterization of a Novel Filarial Serine Protease Inhibitor, Ov-SPI-1, from Onchocerca volvulus, with Potential Multifunctional Roles during Development of the Parasite
Ford, L., Guiliano, D.B., Oksov, Y., Debnath, A.K., Liu, J., Williams, S.A., Blaxter, M.L. and Lustigman, S. 2005. Characterization of a Novel Filarial Serine Protease Inhibitor, Ov-SPI-1, from Onchocerca volvulus, with Potential Multifunctional Roles during Development of the Parasite. Journal of Biological Chemistry. 280 (49), pp. P40845-40856. https://doi.org/10.1074/jbc.m504434200

A molecular map of mesenchymal tumors
Henderson, S.R., Guiliano, D.B., Presneau, N., McLean, S., Frow, R., Vujovic, S., Anderson, J., Sebire, N., Whelan, J., Athanasou, N., Flanagan, A.M. and Boshoff, C. 2005. A molecular map of mesenchymal tumors. Genome Biology. 6 R76. https://doi.org/10.1186/gb-2005-6-9-r76

Construction of bacterial artificial chromosome libraries from the parasitic nematode Brugia malayi and physical mapping of the genome of its Wolbachia endosymbiont
Foster, J.M., Kumar, S., Ganatra, M.B., Kamal, I.H., Ware, J., Ingram, J., Pope-Chappell, J., Guiliano, D.B., Whitton, C., Daub, J., Blaxter, M.L. and Slatko, B.E. 2004. Construction of bacterial artificial chromosome libraries from the parasitic nematode Brugia malayi and physical mapping of the genome of its Wolbachia endosymbiont. International Journal for Parasitology. 34 (6), pp. 733-746. https://doi.org/10.1016/j.ijpara.2004.02.001

A gene family of cathepsin L-like proteases of filarial nematodes are associated with larval molting and cuticle and eggshell remodeling
Guiliano, D.B., Hong, X., McKerrow, J.H., Blaxter, M.L., Oksov, Y., Liu, J., Ghedin, E. and Lustigman, S. 2004. A gene family of cathepsin L-like proteases of filarial nematodes are associated with larval molting and cuticle and eggshell remodeling. Molecular and Biochemical Parasitology. 36 (2), pp. 227-242. https://doi.org/10.1016/j.molbiopara.2004.03.015

Most of the Response Elicited against Wolbachia Surface Protein in Filarial Nematode Infection Is Due to the Infective Larval Stage
Lamb, T.J., Le Goff, L., Kurniawan, A., Guiliano, D.B., Fenn, K., Blaxter, M.L., Read, A.F. and Allen, J.E. 2004. Most of the Response Elicited against Wolbachia Surface Protein in Filarial Nematode Infection Is Due to the Infective Larval Stage. The Journal of Infectious Diseases. 189 (1), pp. 120-127. https://doi.org/10.1086/380490

Cathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans
Hashmi, S., Britton, C., Liu, J., Guiliano, D.B., Oksov, Y. and Lustigman, S. 2002. Cathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans. Journal of Biological Chemistry. 277 (5), pp. P3477-3486. https://doi.org/10.1074/jbc.m106117200

The Brugia malayi genome project: expressed sequence tags and gene discovery
Blaxter, M., Daub, J., Guiliano, D.B., Parkinson, J., Whitton, C. and The Filarial Genome Project 2002. The Brugia malayi genome project: expressed sequence tags and gene discovery. Transactions of the Royal Society of Tropical Medicine and Hygiene. 96 (1), pp. 7-17. https://doi.org/10.1016/s0035-9203(02)90224-5

Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi
Ware, J., Moran, L., Foster, J., Posfai, J., Vincze, T., Guiliano, D.B., Blaxter, M., Eisen, J. and Slatko, B. 2002. Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi. International Journal for Parasitology. 32 (2), pp. 159-166. https://doi.org/10.1016/s0020-7519(01)00367-8

IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype
Loke, P., Nair, M.G., Parkinson, J., Guiliano, D.B., Blaxter, M. and Allen, J.E. 2002. IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype. BMC Immunology. 3 7. https://doi.org/10.1186/1471-2172-3-7

Making sense of EST sequences by CLOBBing them
Parkinson, J., Guiliano, D.B. and Blaxter, M. 2002. Making sense of EST sequences by CLOBBing them. BMC Bioinformatics. 3 31. https://doi.org/10.1186/1471-2105-3-31

Conservation of long-range synteny and microsynteny between the genomes of two distantly related nematodes
Guiliano, D.B., Hall, N., Jones, S.J.M., Clark, L.N., Corton, C.H., Barrell, B.G. and Blaxter, M.L. 2002. Conservation of long-range synteny and microsynteny between the genomes of two distantly related nematodes. Genome Biology. 3 (10) research0057.1. https://doi.org/10.1186/gb-2002-3-10-research0057

200 000 nematode expressed sequence tags on the Net
Parkinson, J., Whitton, C., Guiliano, D.B., Daub, J. and Blaxter, M. 2001. 200 000 nematode expressed sequence tags on the Net. Trends in Parasitology. 17 (8), pp. P394-396. https://doi.org/10.1016/s1471-4922(01)01954-7

The filarial genome project: analysis of the nuclear, mitochondrial and endosymbiont genomes of Brugia malayi
Williams, S.A., Lizotte-Waniewski, M.R., Foster, J., Guiliano, D.B., Daub, J., Scott, A.L., Slatko, B. and Blaxter, M.L. 2000. The filarial genome project: analysis of the nuclear, mitochondrial and endosymbiont genomes of Brugia malayi. International Journal for Parasitology. 30 (4), pp. 411-419. https://doi.org/10.1016/s0020-7519(00)00014-x

Analysis of Genes Expressed at the Infective Larval Stage Validates Utility of Litomosoides sigmodontis as a Murine Model for Filarial Vaccine Development
Judith E. Allen, J.E., Jennifer Daub, J., Guiliano, D.B., McDonnell, A., Lizotte-Waniewski, M., Taylor, D.W. and Blaxter, M. 2000. Analysis of Genes Expressed at the Infective Larval Stage Validates Utility of Litomosoides sigmodontis as a Murine Model for Filarial Vaccine Development. Infection and Immunity. 68 (9), pp. 5454-5458. https://doi.org/10.1128/iai.68.9.5454-5458.2000

Identification of Potential Vaccine and Drug Target Candidates by Expressed Sequence Tag Analysis and Immunoscreening of Onchocerca volvulus Larval cDNA Libraries
Lizotte-Waniewski, M., Tawe, W., Guiliano, D.B., Lu, W., Liu, J., Williams, S.A. and Lustigman, S. 2000. Identification of Potential Vaccine and Drug Target Candidates by Expressed Sequence Tag Analysis and Immunoscreening of Onchocerca volvulus Larval cDNA Libraries. Infection and Immunity. 68 (6), pp. 3491-3501. https://doi.org/10.1128/iai.68.6.3491-3501.2000

Chemiluminescent Detection of Sequential DNA Hybridizations to High-Density, Filter-Arrayed cDNA Libraries: A Subtraction Method for Novel Gene Discovery
Guiliano, D.B., Ganatra, M., Ware, J., Parrot, J., Daub, J., Moran, L., Brennecke, H., Foster, J.M., Supali, T., Blaxter, M., Scott, A.L., Williams, S.A. and Slatko, B.E. 1999. Chemiluminescent Detection of Sequential DNA Hybridizations to High-Density, Filter-Arrayed cDNA Libraries: A Subtraction Method for Novel Gene Discovery. BioTechniques. 27 (1), pp. 146-152. https://doi.org/10.2144/99271rr03

Parasitic helminth genomics
Blaxter, M., M. Aslett, M., Guiliano, D.B., Daub, J. and The Filarial Genome Project 1999. Parasitic helminth genomics. Parasitology. 118 (7), pp. 39-51. https://doi.org/10.1017/s0031182099004060

A unified nomenclature for filarial genes
Blaxter, M.L., Guiliano, D.B., Scott, A.L. and Williams, S.A. 1997. A unified nomenclature for filarial genes. Parasitology Today. 13 (11), pp. P416-417. https://doi.org/10.1016/s0169-4758(97)01140-x

Genes expressed in Brugia malayi infective third stage larvae
Blaxter, M.L., Raghavan, N., Ghosh, I., Guiliano, D.B., Lu, W., Williams, S.A., Slatko, B. and Scott, A.L. 1996. Genes expressed in Brugia malayi infective third stage larvae. Molecular and Biochemical Parasitology. 77 (1), pp. 77-93. https://doi.org/10.1016/0166-6851(96)02571-6