Cathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans

Hashmi, S., Britton, C., Liu, J., Guiliano, D.B., Oksov, Y. and Lustigman, S. 2002. Cathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans. Journal of Biological Chemistry. 277 (5), pp. P3477-3486. https://doi.org/10.1074/jbc.m106117200

TitleCathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans
TypeJournal article
AuthorsHashmi, S., Britton, C., Liu, J., Guiliano, D.B., Oksov, Y. and Lustigman, S.
Abstract

Cysteine proteases play critical biological roles in both intracellular and extracellular processes. We characterizedCe-cpl-1, a Caenorhabditis elegans cathepsin L-like cysteine protease. RNA interference with Ce-cpl-1activity resulted in embryonic lethality and a transient delayed growth of larvae to egg producing adults, suggesting an essential role forcpl-1 during embryogenesis, and most likely during post-embryonic development. Cpl-1 gene (Ce-cpl-1:lacZ) is widely expressed in the intestine and hypodermal cells of transgenic worms, while the fusion protein (Ce-CPL-1::GFP) was expressed in the hypodermis, pharynx, and gonad. The CPL-1 native protein accumulates in early to late stage embryos and becomes highly concentrated in gut cells during late embryonic development. CPL-1 is also present near the periphery of the eggshell as well as in the cuticle of larval stages suggesting that it may function not only in embryogenesis but also in further development of the worm. Although the precise role of Ce-CPL-1 during embryogenesis is not yet clear it could be involved in the processing of nutrients responsible for synthesis and/or in the degradation of eggshell. Moreover, an increase in the cpl-1 mRNA is seen in the intermolt period approximately 4 h prior to each molt. During this process Ce-CPL-1 may act as a proteolytic enzyme in the processing/degradation of cuticular or other proteins. Similar localization of a related cathepsin L in the filarial nematodeOnchocerca volvulus, eggshell and cuticle, suggests that some of the Ce-CPL-1 function during development may be conserved in other parasitic nematodes.

JournalJournal of Biological Chemistry
Journal citation277 (5), pp. P3477-3486
ISSN0021-9258
Year2002
PublisherElsevier
Digital Object Identifier (DOI)https://doi.org/10.1074/jbc.m106117200
Web address (URL)http://dx.doi.org/10.1074/jbc.m106117200
Publication dates
PublishedFeb 2002

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