Operon Conservation and the Evolution of trans-Splicing in the Phylum Nematoda

Guiliano, D.B. and Blaxter, M.L. 2006. Operon Conservation and the Evolution of trans-Splicing in the Phylum Nematoda. PLoS Genetics. 2 (11) e198. https://doi.org/10.1371/journal.pgen.0020198

TitleOperon Conservation and the Evolution of trans-Splicing in the Phylum Nematoda
TypeJournal article
AuthorsGuiliano, D.B. and Blaxter, M.L.
Abstract

The nematode Caenorhabditis elegans is unique among model animals in that many of its genes are cotranscribed as polycistronic pre-mRNAs from operons. The mechanism by which these operonic transcripts are resolved into mature mRNAs includes trans-splicing to a family of SL2-like spliced leader exons. SL2-like spliced leaders are distinct from SL1, the major spliced leader in C. elegans and other nematode species. We surveyed five additional nematode species, representing three of the five major clades of the phylum Nematoda, for the presence of operons and the use of trans-spliced leaders in resolution of polycistronic pre-mRNAs. Conserved operons were found in Pristionchus pacificus, Nippostrongylus brasiliensis, Strongyloides ratti, Brugia malayi, and Ascaris suum. In nematodes closely related to the rhabditine C. elegans, a related family of SL2-like spliced leaders is used for operonic transcript resolution. However, in the tylenchine S. ratti operonic transcripts are resolved using a family of spliced leaders related to SL1. Non-operonic genes in S. ratti may also receive these SL1 variants. In the spirurine nematodes B. malayi and A. suum operonic transcripts are resolved using SL1. Mapping these phenotypes onto the robust molecular phylogeny for the Nematoda suggests that operons evolved before SL2-like spliced leaders, which are an evolutionary invention of the rhabditine lineage.

Article numbere198
JournalPLoS Genetics
Journal citation2 (11)
ISSN1553-7390
1553-7404
Year2006
PublisherPublic Library of Science
Digital Object Identifier (DOI)https://doi.org/10.1371/journal.pgen.0020198
Web address (URL)http://dx.doi.org/10.1371/journal.pgen.0020198
Publication dates
Published24 Nov 2006

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