Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB - E. coli cytoplasm

Next generation e-learning platform: virtual laboratory simulations
Smith, C.L. and Markiv, A. 2016. Next generation e-learning platform: virtual laboratory simulations. Heads of Biological Sciences, Royal Society of Biology, Spring 2016. University of Leicester 04 - 05 May 2016

Immunocytochemical staining of endogenous nuclear proteins with the HIS-1 anti-poly-histidine monoclonal antibody: a potential source of error in His-tagged protein detection
Chilumuri, A., Markiv, A. and Milton, N.G.N. 2014. Immunocytochemical staining of endogenous nuclear proteins with the HIS-1 anti-poly-histidine monoclonal antibody: a potential source of error in His-tagged protein detection. Acta Histochemica. 116 (6), pp. 1022-1028. https://doi.org/10.1016/j.acthis.2014.04.006

Identification of metastasis-associated glycoproteins in colorectal cancer
Peiris, D., Markiv, A., Curley, G.P. and Dwek, M. 2012. Identification of metastasis-associated glycoproteins in colorectal cancer. European Journal of Cancer. 48 (5), pp. S39-S40. https://doi.org/10.1016/S0959-8049(12)70863-8

Ribosome display of combinatorial antibody libraries derived from mice immunised with heat-killed Xylella fastidiosa and the selection of MopB-specific single-chain antibodies
Azizi, A., Arora, A., Markiv, A., Lampe, D.J., Miller, T. and Kang, A.S. 2012. Ribosome display of combinatorial antibody libraries derived from mice immunised with heat-killed Xylella fastidiosa and the selection of MopB-specific single-chain antibodies. Applied and Environmental Microbiology. 78 (8), pp. 2638-2647. https://doi.org/10.1128/AEM.07807-11

Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune to the biocontrol fungus, Trichoderma viride
Ujor, V.C., Peiris, D., Monti, M., Kang, A.S., Clements, M.O. and Hedger, J.N. 2012. Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune to the biocontrol fungus, Trichoderma viride. Letters in Applied Microbiology. 54 (4), pp. 336-343. https://doi.org/10.1111/j.1472-765X.2012.03215.x

A novel approach to determining the affinity of protein-carbohydrate interactions employing adherent cancer cells grown on a biosensor surface
Peiris, D., Markiv, A., Curley, G.P. and Dwek, M. 2012. A novel approach to determining the affinity of protein-carbohydrate interactions employing adherent cancer cells grown on a biosensor surface. Biosensors and Bioelectronics. 35 (1), pp. 160-166. https://doi.org/10.1016/j.bios.2012.02.037

Beyond the genome and proteome: targeting protein modifications in cancer
Markiv, A., Rambaruth, N.D.S. and Dwek, M. 2012. Beyond the genome and proteome: targeting protein modifications in cancer. Current Opinions in Pharmacology.. 12 (4), pp. 408-413. https://doi.org/10.1016/j.coph.2012.04.003

A physiological approach to assess the affinity of lectin carbohydrate interactions using cancer cells immobilised on a biosensor surface
Peiris, D., Markiv, A. and Dwek, M. 2011. A physiological approach to assess the affinity of lectin carbohydrate interactions using cancer cells immobilised on a biosensor surface. Glycobiology. 21 (11), p. 1523. https://doi.org/10.1093/glycob/cwr126

Identification, cloning and characterization of two N-acetylgalactosamine binding lectins from the albumen gland of Helix pomatia
Markiv, A., Peiris, D., Curley, G.P., Odell, M. and Dwek, M. 2011. Identification, cloning and characterization of two N-acetylgalactosamine binding lectins from the albumen gland of Helix pomatia. Journal of Biological Chemistry. 286 (23), pp. 20260-20266. https://doi.org/10.1074/jbc.M110.184515

Module based antibody engineering: a novel synthetic REDantibody
Markiv, A., Anani, B., Durvasula, R.V. and Kang, A.S. 2011. Module based antibody engineering: a novel synthetic REDantibody. Journal of Immunological Methods. 364 (1-2), pp. 40-49. https://doi.org/10.1016/j.jim.2010.10.009

Fluorescent fusion polypeptides and methods of use
Markiv, A., Durvasula, R.V. and Kang, A.S. 2011. Fluorescent fusion polypeptides and methods of use.

Trypanosoma cruzi: Synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to T. cruzi and potential bacterial hosts
Fiecka, A., Hurwitza, I., Kang, A.S. and Durvasula, R.V. 2010. Trypanosoma cruzi: Synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to T. cruzi and potential bacterial hosts. Experimental Parasitology. 125 (4), pp. 342-347. https://doi.org/10.1016/j.exppara.2010.02.016

Cancer prognostication using a recombinant form of the lectin from Helix pomatia agglutinin
Dwek, M., Markiv, A. and Odell, M. 2009. Cancer prognostication using a recombinant form of the lectin from Helix pomatia agglutinin. Glycobiology. 19 (11), p. 1318. https://doi.org/10.1093/glycob/cwp135

Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals
Nguyen, T.V., Sacci, J.B., de la Vega, P., John, C.C., James, A.A. and Kang, A.S. 2009. Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals. Malaria Journal. 8, p. 235. https://doi.org/10.1186/1475-2875-8-235

Antibodies against protective antigen and methods of use for passive immunization and treatment of anthrax
Jeanne, Wang, F., Jiang, I., Sawada-Hirai, R., Scholz, W.W., Morrow, P.R. and Kang, A.S. 2008. Antibodies against protective antigen and methods of use for passive immunization and treatment of anthrax.

Immunization and treatment methods for anthrax
Jeanne, Wang, F., Jiang, I., Sawada-Hirai, R., Scholz, W.W., Morrow, P.R. and Kang, A.S. 2008. Immunization and treatment methods for anthrax.

Microbial biosynthesis of PHAs and its applications
Roy, I., Philip, S.E., Francis, L., Rai, R., Valappil, S.P., Markiv, A., Keshavarz, T., Misra, S.K., Decheng, M. and Boccaccini, A.R. 2008. Microbial biosynthesis of PHAs and its applications. COST Action 868: Biotechnical Functionalisation of Renewable Polymeric Materials: 2008 Annual Workshop (combined Working Group and Management Committee meeting). Varna, Bulgaria 18 -19 Sep 2008

Microbial PHA biosynthesis and its applications
Roy, I., Philip, S.E., Francis, L., Rai, R., Valappil, S.P., Markiv, A., Keshavarz, T., Misra, S.K., Decheng, M. and Boccaccini, A.R. 2008. Microbial PHA biosynthesis and its applications. 11th International Symposium on Biodegradable Polyesters. Massey University in Auckland, New Zealand 23 - 26 Nov 2008

The three-dimensional structure of codakine and related marine C-type lectins
Gourdine, J.P., Markiv, A. and Smith-Ravin, J. 2007. The three-dimensional structure of codakine and related marine C-type lectins. Fish & Shellfish Immunology. 23 (4), pp. 831-839. https://doi.org/10.1016/j.fsi.2007.03.009

Human monoclonal anti-protective antigen antibody completely protects rabbits and is synergistic with ciprofloxacin in protecting mice and guinea pigs against inhalation anthrax
Peterson, J.W., Comer, J.E., Noffsinger, D.M., Wenglikowski, A., Walberg, K.G., Chatuev, B.M., Chopra, A.K., Stanberry, L.R., Kang, A.S., Scholz, W.W. and Sircar, J. 2006. Human monoclonal anti-protective antigen antibody completely protects rabbits and is synergistic with ciprofloxacin in protecting mice and guinea pigs against inhalation anthrax. Infection and Immunity. 74 (2), pp. 1016-1024. https://doi.org/10.1128/IAI.74.2.1016-1024.2006

The detection of protective antigen (PA) associated with spores of Bacillus anthracis and the effects of anti-PA antibodies on spore germination and macrophage interactions
Cote, C.K., Rossi, C.A., Kang, A.S., Morrow, P.R., Lee, J.S. and Welkos, S.L. 2005. The detection of protective antigen (PA) associated with spores of Bacillus anthracis and the effects of anti-PA antibodies on spore germination and macrophage interactions. Microbial Pathogenesis. 38 (5-6), pp. 209-225. https://doi.org/10.1016/j.micpath.2005.02.001

Cognate peptide-receptor ligand mapping by directed phage display
Stratmann, T. and Kang, A.S. 2005. Cognate peptide-receptor ligand mapping by directed phage display. Proteome Science. 3 (7). https://doi.org/10.1186/1477-5956-3-7

Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor
Chappel, J.A., Rogers, W.O., Hoffman, S.L. and Kang, A.S. 2004. Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor. Malaria Journal. 3 (28). https://doi.org/10.1186/1475-2875-3-28

Human anti-anthrax protective antigen neutralizing monoclonal antibodies derived from donors vaccinated with anthrax vaccine adsorbed
Sawada-Hirai, R., Jiang, I., Wang, F., Sun, S.M., Nedellec, R., Ruther, P., Alvarez, A., Millis, D., Morrow, P.R. and Kang, A.S. 2004. Human anti-anthrax protective antigen neutralizing monoclonal antibodies derived from donors vaccinated with anthrax vaccine adsorbed. Journal of Immune Based Therapies and Vaccines. 2 (5). https://doi.org/10.1186/1476-8518-2-5

Human monoclonal antibodies that neutralize anthrax toxin by inhibiting heptamer assembly
Wang, F., Ruther, P., Jiang, I., Sawada-Hirai, R., Sun, S.M., Nedellec, R., Morrow, P.R. and Kang, A.S. 2004. Human monoclonal antibodies that neutralize anthrax toxin by inhibiting heptamer assembly. Human Antibodies. 13 (4), pp. 105-110.

IgG_{4} Pf NPNA-1 a human anti-Plasmodium falciparum sporozoite monoclonal antibody cloned from a protected individual inhibits parasite invasion of hepatocytes
Chappel, J.A., Hollingdale, M.R. and Kang, A.S. 2004. IgG_{4} Pf NPNA-1 a human anti-Plasmodium falciparum sporozoite monoclonal antibody cloned from a protected individual inhibits parasite invasion of hepatocytes. Human Antibodies. 13 (3), pp. 91-96.

Stage-dependent localization of a novel gene product of the malaria parasite, Plasmodium falciparum
Nguyen, T.V., Fujioka, H., Kang, A.S., Rogers, W.O., Fidock, D.A. and James, A.A. 2001. Stage-dependent localization of a novel gene product of the malaria parasite, Plasmodium falciparum. Journal of Biological Chemistry. 276 (28), pp. 26724-26731. https://doi.org/10.1074/jbc.M103375200

The I-Ag7 MHC class II molecule linked to murine diabetes is a promiscuous peptide binder
Stratmann, T., Apostolopoulos, V., Mallet-Designe, V., Corper, A.L., Scott, C.A., Wilson, I.A., Kang, A.S. and Teyton, L. 2000. The I-Ag7 MHC class II molecule linked to murine diabetes is a promiscuous peptide binder. Journal of Immunology. 165 (6), pp. 3214-3225.

A structural framework for deciphering the link between I-Ag7 and autoimmune diabetes
Corper, A.L., Stratmann, T., Apostolopoulos, V., Scott, C.A., Garcia, K.C., Kang, A.S., Wilson, I.A. and Teyton, L. 2000. A structural framework for deciphering the link between I-Ag7 and autoimmune diabetes. Science. 288 (5465), pp. 505-511.

Expression of a functional antibody fragment in the gut of Rhodnius prolixus via transgenic bacterial symbiont Rhodococcus rhodnii
Durvasula, R.V., Gumbs, A., Panackal, A., Kruglov, O., Taneja, J., Kang, A.S., Cordon-Rosales, C., Richards, F.F., Whitham, R.G. and Beard, C.B. 1999. Expression of a functional antibody fragment in the gut of Rhodnius prolixus via transgenic bacterial symbiont Rhodococcus rhodnii. Medical and Veterinary Entomology. 13 (2), pp. 115-119. https://doi.org/10.1046/j.1365-2915.1999.00175.x

Modulation of antibody display on M13 filamentous phage
Chappel, J.A., He, M. and Kang, A.S. 1998. Modulation of antibody display on M13 filamentous phage. Journal of Immunological Methods. 221 (1-2), pp. 25-34. https://doi.org/10.1016/S0022-1759(98)00094-5

Recombinant immunocytokines targeting the mouse transferrin receptor: construction and biological activities
Dreier, T., Lode, H.N., Xiang, R., Dolman, C.S., Reisfeld, R.A. and Kang, A.S. 1998. Recombinant immunocytokines targeting the mouse transferrin receptor: construction and biological activities. Bioconjugate Chemistry. 9 (4), pp. 482-489. https://doi.org/10.1021/bc980020e

Gene therapy with a single chain interleukin 12 fusion protein induces T cell-dependent protective immunity in a syngeneic model of murine neuroblastoma
Lode, H.N., Dreier, T., Xiang, R., Varki, N.M., Kang, A.S. and Reisfeld, R.A. 1998. Gene therapy with a single chain interleukin 12 fusion protein induces T cell-dependent protective immunity in a syngeneic model of murine neuroblastoma. Proceedings of the National Academy of Sciences. 95 (5), pp. 2475-2480.

Bacterial expression and purification of recombinant Plasmodium yoelii circumsporozoite protein
Stratmann, T., Schmida, S.R., Harperb, J.F. and Kang, A.S. 1997. Bacterial expression and purification of recombinant Plasmodium yoelii circumsporozoite protein. Protein Expression and Purification. 11 (1), pp. 72-78. https://doi.org/10.1006/prep.1997.0768

Cloning and expression of a single-chain antibody fragment specific for foot-and-mouth disease virus
Mason, P.W., Berinstein, A., Baxt, B., Parsells, R., Kang, A.S. and Reider, E. 1996. Cloning and expression of a single-chain antibody fragment specific for foot-and-mouth disease virus. Virology. 224 (2), pp. 548-554. https://doi.org/10.1006/viro.1996.0562

Propagation of an attenuated virus by design: Engineering a novel receptor for a noninfectious foot-and-mouth disease virus
Rieder, E., Berinstein, A., Baxt, B., Kang, A.S. and Mason, P.W. 1996. Propagation of an attenuated virus by design: Engineering a novel receptor for a noninfectious foot-and-mouth disease virus. Proceedings of the National Academy of Sciences. 91 (19), pp. 10428-10433.

Functional expression of a single-chain anti-progesterone antibody fragment in the cytoplasm of a mutant Escherichia coil
He, M., Hamon, M., Liu, H., Kang, A.S. and Taussig, M.J. 1995. Functional expression of a single-chain anti-progesterone antibody fragment in the cytoplasm of a mutant Escherichia coil. Nucleic Acids Research. 23 (19), pp. 4009-4010. https://doi.org/10.1093/nar/23.19.4009

Characterization of a progesterone-binding, three-domain antibody fragment (VH/K) expressed in Escherichia coli
He, M., Kang, A.S., Hamon, M., Humphreys, A.S., Gani, M. and Taussig, M.J. 1995. Characterization of a progesterone-binding, three-domain antibody fragment (VH/K) expressed in Escherichia coli. Immunology. 84 (4), pp. 662-668.

Properties of a three domain anti-progesterone antibody fragment expressed in Escherichia coli: comparison of periplasmic, culture medium and refolded forms
He, M., Hamon, M., Liu, H., Gani, M., Kang, A.S. and Taussig, M.J. 1995. Properties of a three domain anti-progesterone antibody fragment expressed in Escherichia coli: comparison of periplasmic, culture medium and refolded forms. Tumor Targeting. 1, pp. 267-274.

Crystallization, sequence and preliminary crystallographic data for transmission-blocking anti-malaria Fab 4B7 with cyclic peptides from the Pfs25 protein of P. falciparum
Stura, E.A., Kang, A.S., Stefanko, R.S., Calvo, J.C., Kaslow, D.C. and Satterthwait, A.C. 1994. Crystallization, sequence and preliminary crystallographic data for transmission-blocking anti-malaria Fab 4B7 with cyclic peptides from the Pfs25 protein of P. falciparum. Acta Crystallographica. D50 (4), pp. 535-542. https://doi.org/10.1107/S0907444994001356

Antibodies without immunization
Lerner, R.A., Kang, A.S., Bain, J.D., Burton, D.R. and Barbas III, C.F. 1992. Antibodies without immunization. Science. 258 (5086), pp. 1313-1314. https://doi.org/10.1126/science.1455226

In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library
Gram, H., Marconi, L.A., Barbas III, C.F., Collet, T.A., Lerner, R.A. and Kang, A.S. 1992. In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library. Proceedings of the National Academy of Sciences. 89 (8), pp. 3576-3580.

Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries
Kang, A.S., Jones, T.M. and Burton, D.R. 1991. Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries. Proceedings of the National Academy of Sciences. 88 (24), pp. 11120-11123.

On the use of combinatorial antibody libraries to clone the "fossil record" of an individual's immune response
Lerner, R.A., Barbas III, C.F., Kang, A.S. and Burton, D.R. 1991. On the use of combinatorial antibody libraries to clone the "fossil record" of an individual's immune response. Proceedings of the National Academy of Sciences. 88 (21), pp. 9705-9706.

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site
Barbas III, C.F., Kang, A.S., Lerner, R.A. and Benkovic, S.J. 1991. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proceedings of the National Academy of Sciences. 88 (18), pp. 7978-7982.

Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces
Kang, A.S., Barbas III, C.F., Janda, K.J., Benkovic, S.J. and Lerner, R.A. 1991. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. Proceedings of the National Academy of Sciences. 88 (10), pp. 4363-4366.

Combinatorial immunoglobulin libraries in phage
Kang, A.S., Burton, D.R. and Lerner, R.A. 1991. Combinatorial immunoglobulin libraries in phage. Methods: a Companion to Methods in Enzymology. 2 (2), pp. 111-118. https://doi.org/10.1016/S1046-2023(05)80211-7

Catalytic antibodies-designer enzymes
Kang, A.S., Kingsbury, G.A., Blackburn, G.M. and Burton, D.R. 1990. Catalytic antibodies-designer enzymes. Chemistry in Britain.

Catalytic antibodies
Kang, A.S., Burton, D.R. and Blackburn, G.M. 1990. Catalytic antibodies. Analytical Proceedings. 27, pp. 6-12.

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
Huse, W.D., Sastry, L., Iverson, S.A., Kang, A.S., Alting-Mees, M., Burton, D.R., Benkovic, S.J. and Lerner, R.A. 1989. Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science. 246 (4935), pp. 1275-1281. https://doi.org/10.1126/science.2531466

Low-resolution epitope characterisation in a family of seed apolipoproteins using polyclonal antibodies
Au, D.M.Y., Kang, A.S. and Murphy, D.J. 1989. Low-resolution epitope characterisation in a family of seed apolipoproteins using polyclonal antibodies. Biochimica et Biophysica Acta: Lipids and Lipid Metabolism. 1005 (1), pp. 97-102. https://doi.org/10.1016/0005-2760(89)90037-4

An immunologically related family of apolipoproteins associated with triacylglycerol storage in the Cruciferae
Au, D.M.Y., Kang, A.S. and Murphy, D.J. 1989. An immunologically related family of apolipoproteins associated with triacylglycerol storage in the Cruciferae. Archives of Biochemistry and Biophysics. 273 (2), pp. 516-526. https://doi.org/10.1016/0003-9861(89)90511-0

Catalytic antibodies
Blackburn, G.M., Kang, A.S., Kingsbury, G.A. and Burton, D.R. 1989. Catalytic antibodies. Biochemical Journal. 262 (2), pp. 381-390.

Synthesis of the major oil-body membrane protein in developing rapeseed (Brassica napus) embryos. Integration with storage-lipid and storage-protein synthesis and implications for the mechanism of oil-body formation
Murphy, D.J., Cummins, I. and Kang, A.S. 1989. Synthesis of the major oil-body membrane protein in developing rapeseed (Brassica napus) embryos. Integration with storage-lipid and storage-protein synthesis and implications for the mechanism of oil-body formation. Biochemical Journal. 258 (1), pp. 285-293.

Immunological investigation of lipases in germinating oilseed rape, Brassica napus
Murphy, D.J., Cummins, I. and Kang, A.S. 1989. Immunological investigation of lipases in germinating oilseed rape, Brassica napus. Journal of the Science of Food and Agriculture. 47, pp. 21-31.

ELISA of aflatoxins
Wilkinson, A.P., Kang, A.S., Chan, H.W.S. and Morgan, M.R.A. 1988. ELISA of aflatoxins. in: Morris, B.A., Clifford, M.N. and Jackman, R. (ed.) Immunoassays for veterinary and food analysis - 1 London, UK Elsevier. pp. 343-345

Novel hapten-protein conjugation methods for the synthesis of immunogens and coating conjugates for use in ELISA
Kang, A.S., Chan, H.W.S. and Morgan, M.R.A. 1988. Novel hapten-protein conjugation methods for the synthesis of immunogens and coating conjugates for use in ELISA. in: Morris, B.A., Clifford, M.N. and Jackman, R. (ed.) Immunoassays for veterinary and food analysis - 1 London, UK Elsevier. pp. 271-273

Assay of gene expression by immunodetection
Kang, A.S. and Abbott, S.J. 1988. Assay of gene expression by immunodetection. in: Robins, R.J. and Rhodes, M.J.C. (ed.) Manipulating secondary metabolism in culture Oxford University Press.

Expression of legumin sequences in pea, Nicotiana and yeast
Croy, R.R.D., Evans, I.M., Yarwood, J.N., Harris, N., Gatehouse, J.A., Shirsat, A.H., Kang, A.S., Ellis, J.R., Thompson, A.J. and Boulter, D. 1988. Expression of legumin sequences in pea, Nicotiana and yeast. Biochemie und Physiologie der Pflanzen. 183, pp. 183-197.

Production of antisera against sterigmatocystin and its use in an enzyme-linked immunosorbent assay for sterigmatocystin in barley
Morgan, M.R.A., Kang, A.S. and Chan, H.W.S. 1986. Production of antisera against sterigmatocystin and its use in an enzyme-linked immunosorbent assay for sterigmatocystin in barley. Journal of the Science of Food and Agriculture. 37 (9), pp. 873-880. https://doi.org/10.1002/jsfa.2740370911

Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay
Morgan, M.R.A., Kang, A.S. and Chan, H.W.S. 1986. Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay. Journal of the Science of Food and Agriculture. 37 (9), pp. 908-914. https://doi.org/10.1002/jsfa.2740370913

Effects of nutrition on the interaction of Fusarium oxysporum and Sunn-hemp mosaic virus in cowpea seedlings
Chant, S.R., Gbaja, I.S. and Kang, A.S. 1984. Effects of nutrition on the interaction of Fusarium oxysporum and Sunn-hemp mosaic virus in cowpea seedlings. Tropical Agriculture. 61 (2), pp. 87-91.