Cognate peptide-receptor ligand mapping by directed phage display
Stratmann, T. and Kang, A.S. 2005. Cognate peptide-receptor ligand mapping by directed phage display. Proteome Science. 3 (7). https://doi.org/10.1186/1477-5956-3-7
Stratmann, T. and Kang, A.S. 2005. Cognate peptide-receptor ligand mapping by directed phage display. Proteome Science. 3 (7). https://doi.org/10.1186/1477-5956-3-7
Title | Cognate peptide-receptor ligand mapping by directed phage display |
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Authors | Stratmann, T. and Kang, A.S. |
Abstract | Background: A rapid phage display method for the elucidation of cognate peptide specific ligand for receptors is described. The approach may be readily integrated into the interface of genomic and proteomic studies to identify biologically relevant ligands. Methods: A gene fragment library from influenza coat protein haemagglutinin (HA) gene was constructed by treating HA cDNA with DNAse I to create 50 - 100 bp fragments. These fragments were cloned into plasmid pORFES IV and in-frame inserts were selected. These in-frame fragment inserts were subsequently cloned into a filamentous phage display vector JC-M13-88 for surface display as fusions to a synthetic copy of gene VIII. Two well characterized antibodies, mAb 12CA5 and pAb 07431, directed against distinct known regions of HA were used to pan the library. Results: Two linear epitopes, HA peptide 112 - 126 and 162 - 173, recognized by mAb 12CA5 and pAb 07431, respectively, were identified as the cognate epitopes. Conclusion: This approach is a useful alternative to conventional methods such as screening of overlapping synthetic peptide libraries or gene fragment expression libraries when searching for precise peptide protein interactions, and may be applied to functional proteomics. |
Journal | Proteome Science |
Journal citation | 3 (7) |
ISSN | 1477-5956 |
Year | 2005 |
Digital Object Identifier (DOI) | https://doi.org/10.1186/1477-5956-3-7 |
Web address (URL) | http://www.proteomesci.com/content/3/1/7 |
Publication dates | |
Published | 2005 |
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Ribosome display of combinatorial antibody libraries derived from mice immunised with heat-killed Xylella fastidiosa and the selection of MopB-specific single-chain antibodies
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Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune to the biocontrol fungus, Trichoderma viride
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Module based antibody engineering: a novel synthetic REDantibody
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Fluorescent fusion polypeptides and methods of use
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Trypanosoma cruzi: Synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to T. cruzi and potential bacterial hosts
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Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals
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Antibodies against protective antigen and methods of use for passive immunization and treatment of anthrax
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Immunization and treatment methods for anthrax
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Human monoclonal anti-protective antigen antibody completely protects rabbits and is synergistic with ciprofloxacin in protecting mice and guinea pigs against inhalation anthrax
Peterson, J.W., Comer, J.E., Noffsinger, D.M., Wenglikowski, A., Walberg, K.G., Chatuev, B.M., Chopra, A.K., Stanberry, L.R., Kang, A.S., Scholz, W.W. and Sircar, J. 2006. Human monoclonal anti-protective antigen antibody completely protects rabbits and is synergistic with ciprofloxacin in protecting mice and guinea pigs against inhalation anthrax. Infection and Immunity. 74 (2), pp. 1016-1024. https://doi.org/10.1128/IAI.74.2.1016-1024.2006
The detection of protective antigen (PA) associated with spores of Bacillus anthracis and the effects of anti-PA antibodies on spore germination and macrophage interactions
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Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor
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Human anti-anthrax protective antigen neutralizing monoclonal antibodies derived from donors vaccinated with anthrax vaccine adsorbed
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Human monoclonal antibodies that neutralize anthrax toxin by inhibiting heptamer assembly
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IgG_{4} Pf NPNA-1 a human anti-Plasmodium falciparum sporozoite monoclonal antibody cloned from a protected individual inhibits parasite invasion of hepatocytes
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Stage-dependent localization of a novel gene product of the malaria parasite, Plasmodium falciparum
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The I-Ag7 MHC class II molecule linked to murine diabetes is a promiscuous peptide binder
Stratmann, T., Apostolopoulos, V., Mallet-Designe, V., Corper, A.L., Scott, C.A., Wilson, I.A., Kang, A.S. and Teyton, L. 2000. The I-Ag7 MHC class II molecule linked to murine diabetes is a promiscuous peptide binder. Journal of Immunology. 165 (6), pp. 3214-3225.
A structural framework for deciphering the link between I-Ag7 and autoimmune diabetes
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Expression of a functional antibody fragment in the gut of Rhodnius prolixus via transgenic bacterial symbiont Rhodococcus rhodnii
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Modulation of antibody display on M13 filamentous phage
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Recombinant immunocytokines targeting the mouse transferrin receptor: construction and biological activities
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Gene therapy with a single chain interleukin 12 fusion protein induces T cell-dependent protective immunity in a syngeneic model of murine neuroblastoma
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Bacterial expression and purification of recombinant Plasmodium yoelii circumsporozoite protein
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Cloning and expression of a single-chain antibody fragment specific for foot-and-mouth disease virus
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Propagation of an attenuated virus by design: Engineering a novel receptor for a noninfectious foot-and-mouth disease virus
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Functional expression of a single-chain anti-progesterone antibody fragment in the cytoplasm of a mutant Escherichia coil
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Characterization of a progesterone-binding, three-domain antibody fragment (VH/K) expressed in Escherichia coli
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Properties of a three domain anti-progesterone antibody fragment expressed in Escherichia coli: comparison of periplasmic, culture medium and refolded forms
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Crystallization, sequence and preliminary crystallographic data for transmission-blocking anti-malaria Fab 4B7 with cyclic peptides from the Pfs25 protein of P. falciparum
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Assembly of combinatorial antibody libraries on phage surfaces: the gene III site
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Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces
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Combinatorial immunoglobulin libraries in phage
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Catalytic antibodies-designer enzymes
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Catalytic antibodies
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Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
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Low-resolution epitope characterisation in a family of seed apolipoproteins using polyclonal antibodies
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An immunologically related family of apolipoproteins associated with triacylglycerol storage in the Cruciferae
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Catalytic antibodies
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Synthesis of the major oil-body membrane protein in developing rapeseed (Brassica napus) embryos. Integration with storage-lipid and storage-protein synthesis and implications for the mechanism of oil-body formation
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Immunological investigation of lipases in germinating oilseed rape, Brassica napus
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ELISA of aflatoxins
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Novel hapten-protein conjugation methods for the synthesis of immunogens and coating conjugates for use in ELISA
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Assay of gene expression by immunodetection
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Expression of legumin sequences in pea, Nicotiana and yeast
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Production of antisera against sterigmatocystin and its use in an enzyme-linked immunosorbent assay for sterigmatocystin in barley
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Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay
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Effects of nutrition on the interaction of Fusarium oxysporum and Sunn-hemp mosaic virus in cowpea seedlings
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