Title | Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda |
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Authors | Huse, W.D., Sastry, L., Iverson, S.A., Kang, A.S., Alting-Mees, M., Burton, D.R., Benkovic, S.J. and Lerner, R.A. |
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Abstract | A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies. |
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Journal | Science |
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Journal citation | 246 (4935), pp. 1275-1281 |
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ISSN | 0036-8075 |
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Year | 08 Dec 1989 |
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Digital Object Identifier (DOI) | https://doi.org/10.1126/science.2531466 |
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Publication dates |
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Published | 08 Dec 1989 |
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