Pilot study of the utility and acceptability of tampon sampling for the diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis infections by duplex realtime polymerase chain reaction in United Kingdom sex workers

Kimmitt, P.T., Tabrizi, S.N., Crosatti, M., Garland, S.M., Schober, P.C., Rajakumar, Kumar and Chapman, Cordelia A. 2010. Pilot study of the utility and acceptability of tampon sampling for the diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis infections by duplex realtime polymerase chain reaction in United Kingdom sex workers. International Journal of STD & AIDS. 21 (4), pp. 279-282. https://doi.org/10.1258/ijsa.2009.009277

TitlePilot study of the utility and acceptability of tampon sampling for the diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis infections by duplex realtime polymerase chain reaction in United Kingdom sex workers
AuthorsKimmitt, P.T., Tabrizi, S.N., Crosatti, M., Garland, S.M., Schober, P.C., Rajakumar, Kumar and Chapman, Cordelia A.
Abstract

We aimed to evaluate the acceptability of self-collected tampon samples for the screening of female sex workers for sexually transmitted infections. We recruited 65 sex workers, and 63 agreed to provide tampon samples. The tampon samples were processed by realtime polymerase chain reaction (PCR) targeting Neisseria gonorrhoeae and Chlamydia trachomatis. Urethral and endocervical swabs were also obtained from 61 of 63 participants and tested using culture (N. gonorrhoeae) and the BD ProbeTec strand displacement amplification (SDA) (C. trachomatis) assay. Tampon sampling was preferred by 95% of the women and all favoured being tested away from genitourinary medicine clinics; the most common reasons cited were avoidance of embarrassment (40%) and convenience (30%). Besides near-universal acceptability of tampon sampling, the tampon sampling-PCR approach described in this study appeared to have enhanced sensitivity compared with conventional testing, suggesting the possibility of a residual hidden burden of N. gonorrhoeae and/or C. trachomatis genital infections in UK female sex workers.

JournalInternational Journal of STD & AIDS
Journal citation21 (4), pp. 279-282
ISSN0956-4624
YearApr 2010
PublisherRoyal Society of Medicine Press
Digital Object Identifier (DOI)https://doi.org/10.1258/ijsa.2009.009277
Web address (URL)http://dx.doi.org/10.1258/ijsa.2009.009277
Publication dates
PublishedApr 2010

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