A benzimidazole-based new fluorogenic differential/sequential chemosensor for Cu2+, Zn2+, CN-, P2O74-, DNA, its live-cell imaging and pyrosequencing applications

Anbu, S., Paul, A., Surendranath, K., Shaikh Solaiman, N. and Pombeiro, A.J.L. 2021. A benzimidazole-based new fluorogenic differential/sequential chemosensor for Cu2+, Zn2+, CN-, P2O74-, DNA, its live-cell imaging and pyrosequencing applications. Sensors and Actuators B: Chemical. 337 129785. https://doi.org/10.1016/j.snb.2021.129785

TitleA benzimidazole-based new fluorogenic differential/sequential chemosensor for Cu2+, Zn2+, CN-, P2O74-, DNA, its live-cell imaging and pyrosequencing applications
TypeJournal article
AuthorsAnbu, S., Paul, A., Surendranath, K., Shaikh Solaiman, N. and Pombeiro, A.J.L.
AbstractDifferential chemosensors have emerged as next-generation systems due to their simplicity and favourable responsive properties to produce different signals upon selective binding of various analytes simultaneously. Nevertheless, given their inadequate fluorescence response and laborious synthetic procedures, only a few differential chemosensors have been developed so far. In this work, we have employed a single pot synthesis strategy to establish a new benzimidazole-based Schiff base type fluorogenic chemosensor (DFB) which differentially detects Cu2+ (detection limit (LOD) = 24.4 ± 0.5 nM) and Zn2+ (LOD = 2.18 ± 0.1 nM) through fluorescence “off-on” manner over the library of other metal cations in an aqueous medium. The DFB-derived ‘in situ’ complexes DFB-Cu2+ and DFB-Zn2+ showed fluorescence revival “on-off” responses toward cyanide (CN−) and bio-relevant pyrophosphate (P2O7 4--PPi) ions with a significantly low LOD of 9.43 ± 0.2 and 2.9 ± 0.1 nM, respectively, in water. We have demonstrated the phosphate group-specific binding capability of DFB-Zn2+ , by testing it with both ssDNA and dsDNA samples which displayed fluorescence “turn-off” response (LOD ∼10-7 M), similar to the PPi binding in an aqueous medium, indicating that it interacts explicitly with the phosphate backbone of DNA. We have also harnessed the DFB as a sequential fluorescent probe to detect Cu2+, Zn2+, CN− and P2O7 4- ions in human cervical (HeLa) and breast (MCF-7 and MDA-MB-231 (aggressive and invasive)) cancer cell lines. Moreover, we have explored the PPi recognition capability of DFB-Zn2+ in the polymerase-chain-reaction (PCR) products where PPi is one of the primary by-products during amplification of DNA.
Article number129785
JournalSensors and Actuators B: Chemical
Journal citation337
ISSN0925-4005
Year2021
PublisherElsevier
Accepted author manuscript
License
CC BY-NC-ND 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1016/j.snb.2021.129785
Web address (URL)https://doi.org/10.1016/j.snb.2021.129785
Publication dates
Published15 Jun 2021
ProjectRF19-7464
FunderFundação para a Ciência e Tecnologia (FCT)
Research Fund
Department of Chemistry, the University of Hull, United Kingdom
IST
Page range129785
Licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/

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