An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture

Clark, David J., Moore, Catherine M., Flanagan, Marc, Bocxlaer, Katrien Van, Piperaki, Evangelia-Theophano, Yardley, Vanessa, Croft, Simon L., Tyson, John, Whitehouse, Sam P., O'Halloran, Jonathan, Krishna, Sanjeev and Staines, Henry M. 2022. An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture. BioTechniques. 68 (2), pp. 79-84. https://doi.org/10.2144/btn-2019-0086

TitleAn efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture
TypeJournal article
AuthorsClark, David J., Moore, Catherine M., Flanagan, Marc, Bocxlaer, Katrien Van, Piperaki, Evangelia-Theophano, Yardley, Vanessa, Croft, Simon L., Tyson, John, Whitehouse, Sam P., O'Halloran, Jonathan, Krishna, Sanjeev and Staines, Henry M.
Abstract

The aim of this study was to assess pathogen DNA extraction with a new spin column-based method (DNA-XT). DNA from either whole-blood samples spiked with Plasmodium falciparum or Leishmania donovani amastigote culture was extracted with DNA-XT and compared with that produced by a commercial extraction kit (DNeasy®). Eluates from large and small sample volumes were assessed by PCR and spectroscopy. Using a small volume (5 μl) of blood, the DNA-XT and DNeasy methods produced eluates with similar DNA concentrations (0.63 vs 1.06 ng/μl, respectively). The DNA-XT method produced DNA with lower PCR inhibition than DNeasy. The new technique was also twice as fast and required fewer plastics and manipulations but had reduced total recovered DNA compared with DNeasy.

JournalBioTechniques
Journal citation68 (2), pp. 79-84
ISSN0736-6205
Year2022
PublisherFuture Science
Accepted author manuscript
File Access Level
Open (open metadata and files)
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.2144/btn-2019-0086
Web address (URL)https://europepmc.org/articles/PMC7115988
Publication dates
Published in printFeb 2022
Published online27 Nov 2019

Related outputs

Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants
Catherine M. Moore, Anne Ljungars, Matthew J. Paul, Camilla Holst Dahl, Shirin Ahmadi, Anna Christina Adams, Lise Marie Grav, Sanne Schoffelen, Bjørn Gunnar Voldborg, Andreas Hougaard Laustsen and Julian K-C Ma 2023. Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants. Toxicon. 232 107225. https://doi.org/10.1016/j.toxicon.2023.107225

A Novel Microfluidic Dielectrophoresis Technology to Enable Rapid Diagnosis of Mycobacteria tuberculosis in Clinical Samples
Catherine M. Moore, Jasvir Dhillon, Rebecca Flynn, Krzysztof Gizynski, Candice Adams, George Morgan, David McGurk, Eduardo Boada, Shireen Shabestary, Jonathan Peat, Jonathan O'Halloran, Neil G. Stoker, Philip D. Butcher, Heather Murton and Moore, C. 2023. A Novel Microfluidic Dielectrophoresis Technology to Enable Rapid Diagnosis of Mycobacteria tuberculosis in Clinical Samples. Journal of Molecular Diagnostics. 25 (7), pp. 513-523. https://doi.org/10.1016/j.jmoldx.2023.04.005

Selective Inhibition of Plasmodium falciparum ATPase 6 by Artemisinins and Identification of New Classes of Inhibitors after Expression in Yeast
Moore, Catherine M., Wang, Jigang, Lin, Qingsong, Ferreira, Pedro, Avery, Mitchell A, Elokely, Khaled, Staines, Henry M and Krishna, Sanjeev 2022. Selective Inhibition of Plasmodium falciparum ATPase 6 by Artemisinins and Identification of New Classes of Inhibitors after Expression in Yeast. Antimicrobial Agents and Chemotherapy. 66 (5) e02079-21. https://doi.org/10.1128/aac.02079-21

IgG Seroconversion and Pathophysiology in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
Staines, Henry M., Kirwan, Daniela E., Clark, David J., Adams, Emily R., Augustin, Yolanda, Byrne, Rachel L., Cocozza, Michael, Cubas-Atienzar, Ana I., Cuevas, Luis E., Cusinato, Martina, Davies, Benedict M.O., Davis, Mark, Davis, Paul, Duvoix, Annelyse, Eckersley, Nicholas M., Forton, Daniel, Fraser, Alice J., Garrod, Gala, Hadcocks, Linda, Hu, Qinxue, Johnson, Michael, Kay, Grant A, Klekotko, Kesja, Lewis, Zawditu, Macallan, Derek C., Mensah-Kane, Josephine, Menzies, Stefanie, Monahan, Irene, Moore, Catherine M., Nebe-von-Caron, Gerhard, Owen, Sophie I., Sainter, Chris, Sall, Amadou A., Schouten, James, Williams, Christopher T., Wilkins, John, Woolston, Kevin, Fitchett, Joseph R.A., Krishna, Sanjeev and Planche, Tim 2021. IgG Seroconversion and Pathophysiology in Severe Acute Respiratory Syndrome Coronavirus 2 Infection. Emerging Infectious Diseases. 27 (1), pp. 85-91. https://doi.org/10.3201/eid2701.203074

Characterisation of a highly potent and near pan-neutralising anti-HIV monoclonal antibody expressed in tobacco plants
Moore, Catherine M., Grandits, Melanie, Grünwald-Gruber, Clemens, Altmann, Friedrich, Kotouckova, Maria, Teh, Audrey Y-H and Ma, Julian K-C 2021. Characterisation of a highly potent and near pan-neutralising anti-HIV monoclonal antibody expressed in tobacco plants. Retrovirology. 18 17. https://doi.org/10.1186/s12977-021-00560-6

Preprint: Selective inhibition of PfATP6 by artemisinins and identification of new classes of inhibitors after expression in yeast
Catherine M. Moore, Jigang Wang, Qingsong Lin, Pedro Ferreira, Mitchell A. Avery, Khaled Elokely, Henry M. Staines and Sanjeev Krishna 2021. Preprint: Selective inhibition of PfATP6 by artemisinins and identification of new classes of inhibitors after expression in yeast. biorxiv.org. https://doi.org/10.1101/2021.10.27.466210

Preprint: Rapid development of COVID-19 rapid diagnostics for low resource settings: accelerating delivery through transparency, responsiveness, and open collaboration
Adams, Emily, Augustin, Yolanda, Byrne, Rachel, Clark, David, Cocozza, Michael, Cubas-Atienzar, Ana, Cuevas, Luis, Cusinato, Martina, Davies, Benedict, Davis, Mark, Davis, Paul, Duvoix, Annelyse, Eckersley, Nicholas, Edwards, Thomas, Fletcher, Tom, Fraser, Alice, Garrod, Gala, Hadcocks, Linda, Hu, Qinxue, Johnson, Michael, Kay, Grant, Keymer, Katherine, Kirwan, Daniela, Klekotko, Kesja, Lewis, Zawditu, Mason, Jenifer, Mensah-Kane, Josie, Menzies, Stefanie, Monahan, Irene, Moore, Catherine, Nebe-von-Caron, Gerhard, Owen, Sophie, Planche, Tim, Sainter, Chris, Schouten, James, Staines, Henry, Turtle, Lance, Williams, Chris, Wilkins, John, Woolston, Kevin, Sall, Amadou Alpha, Fitchett, Joseph and Krishna, Sanjeev 2020. Preprint: Rapid development of COVID-19 rapid diagnostics for low resource settings: accelerating delivery through transparency, responsiveness, and open collaboration. medRxiv. https://doi.org/10.1101/2020.04.29.20082099

Preprint: Dynamics of IgG seroconversion and pathophysiology of COVID-19 infections
Staines, Henry, Kirwan, Daniela, Clark, David, Adams, Emily, Augustin, Yolanda, Byrne, Rachel, Cocozza, Michael, Cubas-Atienzar, Ana, Cuevas, Luis, Cusinato, Martina, Davies, Benedict, Davis, Mark, Davis, Paul, Duvoix, Annelyse, Eckersley, Nicholas, Forton, Daniel, Fraser, Alice, Garrod, Gala, Hadcocks, Linda, Hu, Qinxue, Johnson, Michael, Kay, Grant, Klekotko, Kesja, Lewis, Zawditu, Macallan, Derek, Mensah-Kane, Josephine, Menzies, Stefanie, Monahan, Irene, Moore, Catherine, Nebe-von-Caron, Gerhard, Owen, Sophie, Sainter, Chris, Sall, Amadou, Schouten, James, Williams, Chris, Wilkins, John, Woolston, Kevin, Fitchett, Joseph, Krishna, Sanjeev and Planche, Tim 2020. Preprint: Dynamics of IgG seroconversion and pathophysiology of COVID-19 infections. medRxiv. https://doi.org/10.1101/2020.06.07.20124636

Preprint: Characterisation of a Highly Potent and Near Pan-Neutralising Anti-HIV Monoclonal Antibody Expressed in Tobacco Plants
Catherine Margaret Moore, Melanie Grandits, Clemens Grünwald-Gruber, Friedrich Altmann, Maria Kotouckova, Audrey Y.H. Teh and Julian K.C. Ma 2020. Preprint: Characterisation of a Highly Potent and Near Pan-Neutralising Anti-HIV Monoclonal Antibody Expressed in Tobacco Plants. Research Square. https://doi.org/10.21203/rs.3.rs-104508/v1

LGR5 receptor promotes cell-cell adhesion in stem cells and colon cancer cells via the IQGAP1-Rac1 pathway
Carmon, Kendra S, Gong, Xing, Yi, Jing, Wu, Ling, Thomas, Anthony, Moore, Catherine M., Masuho, Ikuo, Timson, David J, Martemyanov, Kirill A and Liu, Qingyun J 2017. LGR5 receptor promotes cell-cell adhesion in stem cells and colon cancer cells via the IQGAP1-Rac1 pathway. The Journal of Biological Chemistry. 292 (36), pp. P14989-15001. https://doi.org/10.1074/jbc.m117.786798

Mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, enlarge the parasite's food vacuole and alter drug sensitivities
Pulcini, Serena, Staines, Henry M., Lee, Andrew H., Shafik, Sarah H., Bouyer, Guillaume, Moore, Catherine M., Daley, Daniel A., Hoke, Matthew J., Altenhofen, Lindsey M., Painter, Heather J., Mu, Jianbing, Ferguson, David J.P., Llinás, Manuel, Martin, Rowena E., Fidock, David A., Cooper, Roland A. and Krishna, Sanjeev 2015. Mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, enlarge the parasite's food vacuole and alter drug sensitivities. Scientific Reports. 5 14552. https://doi.org/10.1038/srep14552

Pumped up: reflections on PfATP6 as the target for artemisinins
Krishna, Sanjeev, Pulcini, Serena, Moore, Catherine M., Teo, Beatrix Huei-Yi and Staines, Henry M. 2014. Pumped up: reflections on PfATP6 as the target for artemisinins. Trends in Pharmacological Sciences. 35 (1), pp. P4-11. https://doi.org/10.1016/j.tips.2013.10.007

Citrate synthase from the liver fluke Fasciola hepatica
Zinsser, Veronika L., Moore, Catherine M., Hoey, Elizabeth M., Trudgett, Alan and Timson, David J. 2013. Citrate synthase from the liver fluke Fasciola hepatica. Parasitology research. 112, pp. 2413-2417. https://doi.org/10.1007/s00436-013-3363-x

Differential expression of liver fluke β-tubulin isotypes at selected life cycle stages
Fuchs, Marc-Aurel, Ryan, Louise A., Chambers, Emma L., Moore, Catherine M., Fairweather, Ian, Trudgett, Alan, Timson, David J., Brennan, Gerry P. and Hoey, Elizabeth M. 2013. Differential expression of liver fluke β-tubulin isotypes at selected life cycle stages. International Journal for Parasitology. 43 (14), pp. 1133-1139. https://doi.org/10.1016/j.ijpara.2013.08.007

A novel calmodulin-like protein from the liver fluke, Fasciola hepatica
Russell, Sean L., McFerran, Neil V., Moore, Catherine M., Tsang, Yvonne, Glass, Peter, Hoey, Elizabeth M., Trudgett, Alan and Timson, David J. 2012. A novel calmodulin-like protein from the liver fluke, Fasciola hepatica. Biochimie . 94 (11), pp. 2398-2406. https://doi.org/10.1016/j.biochi.2012.06.015

A plasma membrane Ca(2+)-ATPase (PMCA) from the liver fluke, Fasciola hepatica
Moore, Catherine M., Hoey, Elizabeth M., Trudgett, Alan and Timson, David J. 2012. A plasma membrane Ca(2+)-ATPase (PMCA) from the liver fluke, Fasciola hepatica. International Journal for Parasitology. 42 (9), pp. 851-858. https://doi.org/10.1016/j.ijpara.2012.06.003

Artemisinins act through at least two targets in a yeast model
Moore, Catherine M., Hoey, Elizabeth M., Trudgett, Alan and Timson, David J. 2011. Artemisinins act through at least two targets in a yeast model. FEMS Yeast Research. 11 (2), pp. 233-237. https://doi.org/10.1111/j.1567-1364.2010.00706.x

Permalink - https://westminsterresearch.westminster.ac.uk/item/w3631/an-efficient-and-novel-technology-for-the-extraction-of-parasite-genomic-dna-from-whole-blood-or-culture


Share this

Usage statistics

27 total views
5 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.