An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture

Clark, David J., Moore, Catherine M., Flanagan, Marc, Bocxlaer, Katrien Van, Piperaki, Evangelia-Theophano, Yardley, Vanessa, Croft, Simon L., Tyson, John, Whitehouse, Sam P., O'Halloran, Jonathan, Krishna, Sanjeev and Staines, Henry M. 2022. An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture. BioTechniques. 68 (2), pp. 79-84. https://doi.org/10.2144/btn-2019-0086

TitleAn efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture
TypeJournal article
AuthorsClark, David J., Moore, Catherine M., Flanagan, Marc, Bocxlaer, Katrien Van, Piperaki, Evangelia-Theophano, Yardley, Vanessa, Croft, Simon L., Tyson, John, Whitehouse, Sam P., O'Halloran, Jonathan, Krishna, Sanjeev and Staines, Henry M.
Abstract

The aim of this study was to assess pathogen DNA extraction with a new spin column-based method (DNA-XT). DNA from either whole-blood samples spiked with Plasmodium falciparum or Leishmania donovani amastigote culture was extracted with DNA-XT and compared with that produced by a commercial extraction kit (DNeasy®). Eluates from large and small sample volumes were assessed by PCR and spectroscopy. Using a small volume (5 μl) of blood, the DNA-XT and DNeasy methods produced eluates with similar DNA concentrations (0.63 vs 1.06 ng/μl, respectively). The DNA-XT method produced DNA with lower PCR inhibition than DNeasy. The new technique was also twice as fast and required fewer plastics and manipulations but had reduced total recovered DNA compared with DNeasy.

JournalBioTechniques
Journal citation68 (2), pp. 79-84
ISSN0736-6205
Year2022
PublisherFuture Science
Accepted author manuscript
File Access Level
Open (open metadata and files)
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.2144/btn-2019-0086
Web address (URL)https://europepmc.org/articles/PMC7115988
Publication dates
Published in printFeb 2022
Published online27 Nov 2019

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