Citrate synthase from the liver fluke Fasciola hepatica

Zinsser, Veronika L., Moore, Catherine M., Hoey, Elizabeth M., Trudgett, Alan and Timson, David J. 2013. Citrate synthase from the liver fluke Fasciola hepatica. Parasitology research. 112, pp. 2413-2417. https://doi.org/10.1007/s00436-013-3363-x

TitleCitrate synthase from the liver fluke Fasciola hepatica
TypeJournal article
AuthorsZinsser, Veronika L., Moore, Catherine M., Hoey, Elizabeth M., Trudgett, Alan and Timson, David J.
Abstract

Citrate synthase catalyses the first step of the Krebs’ tricarboxylic acid cycle. A sequence encoding citrate synthase from the common liver fluke, Fasciola hepatica, has been cloned. The encoded protein sequence is predicted to fold into a largely α-helical protein with high structural similarity to mammalian citrate synthases. Although a hexahistidine-tagged version of the protein could be expressed in Escherichia coli, it was not possible to purify it by nickel-affinity chromatography. Similar results were obtained with a version of the protein which lacks the putative mitochondrial targeting sequence (residues 1 to 29). However, extracts from bacterial cells expressing this version had additional citrate synthase activity after correcting for the endogenous, bacterial activity. The apparent K m for oxaloacetate was found to be 0.22 mM, which is higher than that observed in mammalian citrate synthases. Overall, the sequence and structure of F. hepatica citrate synthase are similar to ones from other eukaryotes, but there are enzymological differences which merit further investigation.

JournalParasitology research
Journal citation112, pp. 2413-2417
ISSN0932-0113
Year2013
PublisherSpringer
Digital Object Identifier (DOI)https://doi.org/10.1007/s00436-013-3363-x
Web address (URL)https://doi.org/10.1007/s00436-013-3363-x
Publication dates
Published14 Mar 2013

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