Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants

Catherine M. Moore, Anne Ljungars, Matthew J. Paul, Camilla Holst Dahl, Shirin Ahmadi, Anna Christina Adams, Lise Marie Grav, Sanne Schoffelen, Bjørn Gunnar Voldborg, Andreas Hougaard Laustsen and Julian K-C Ma 2023. Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants. Toxicon. 232 107225. https://doi.org/10.1016/j.toxicon.2023.107225

TitleCharacterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants
TypeJournal article
AuthorsCatherine M. Moore, Anne Ljungars, Matthew J. Paul, Camilla Holst Dahl, Shirin Ahmadi, Anna Christina Adams, Lise Marie Grav, Sanne Schoffelen, Bjørn Gunnar Voldborg, Andreas Hougaard Laustsen and Julian K-C Ma
Abstract

Current snakebite antivenoms are based on polyclonal animal-derived antibodies, which can neutralize snake venom toxins in envenomed victims, but which are also associated with adverse reactions. Therefore, several efforts within antivenom research aim to explore the utility of recombinant monoclonal antibodies, such as human immunoglobulin G (IgG) antibodies, which are routinely used in the clinic for other indications. In this study, the feasibility of using tobacco plants as bioreactors for expressing full-length human monoclonal IgG antibodies against snake toxins was investigated. We show that the plant-produced antibodies perform similarly to their mammalian cell-expressed equivalents in terms of in vitro binding. Complete neutralization was achieved by both the plant and mammalian cell-produced anti-α-cobratoxin antibody. The feasibility of using plant-based expression systems may potentially make it easier for laboratories in resource-poor settings to work with human monoclonal IgG antibodies.

Article number107225
JournalToxicon
Journal citation232
ISSN0041-0101
1879-3150
Year2023
PublisherElsevier
Accepted author manuscript
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1016/j.toxicon.2023.107225
Web address (URL)https://doi.org/10.1016/j.toxicon.2023.107225
Publication dates
Published online11 Jul 2023
FunderVillum Foundation
European Research Council
Wellcome
Novo Nordisk Foundation

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