Klebsiella pneumoniae subsp. pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman

Hoyles, L., Murphy, J., Neve, H., Mahony, J., Gibson, G.R., McCartney, A.L., van Sinderen, D., Turton, J.F. and Sanderson, J.D. 2015. Klebsiella pneumoniae subsp. pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman. 8th International Yakult Symposium: Probiotics, a protective approach to health – an expert insight into the latest research. Berlin 23 Apr 2015

TitleKlebsiella pneumoniae subsp. pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman
AuthorsHoyles, L., Murphy, J., Neve, H., Mahony, J., Gibson, G.R., McCartney, A.L., van Sinderen, D., Turton, J.F. and Sanderson, J.D.
TypeConference poster
Abstract

A recent study characterizing bacteriophage populations within human caecal effluent demonstrated the presence of numerous Podoviridae, Siphoviridae and Myoviridae within this material (Hoyles et al., 2014, Res Microbiol 165, 803–812). Further to this work, anaerobic bacteria were isolated on fastidious anaerobe agar from the caecal effluent of a healthy 31-year-old woman. Ten colonies were selected at random, streaked to purity and screened against the remaining caecal effluent (filter-sterilized, 0.45 μm pore size) in an attempt to isolate lytic bacteriophages. Bacteriophages within the effluent [2×105 ± 2.65×103 (n=3) pfu/ml] were active against five of the isolates, all identified by 16S rRNA gene sequence analysis as Klebsiella pneumoniae. One of the five isolates, L4-FAA5, was characterized further and found to be K. pneumoniae subsp. pneumoniae capsule type K2 rmpA+, and was used to propagate a bacteriophage (which we named KLPN1) to purity. Bacteriophage KLPN1 was a member of the Siphoviridae with a rosette-like tail tip and exhibited depolymerase activity, demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of 21 clinical strains representing unknown K. pneumoniae subsp. pneumoniae capsule types and types K1, K2, K5, K20, K54 and K57, KLPN1 infected only K2 strains, but did not exhibit depolymerase activity against these. Whole-genome sequence analysis of KLPN1 showed the bacteriophage to have a genome of 49,037 bp (50.53 GC mol%) comprising 73 predicted ORFs, of which 22 encoded genes associated with structure, host recognition, packaging, DNA replication and cell lysis. The host recognition-associated gene was a potential depolymerase. This is the first report of the isolation of a bacterium–bacteriophage combination from the human caecum, and only the third member of the Siphoviridae known to infect K. pneumoniae subsp. pneumoniae.

Year2015
Conference8th International Yakult Symposium: Probiotics, a protective approach to health – an expert insight into the latest research
Accepted author manuscript
Web address (URL) of conference proceedingshttp://yakultsymposium.com/programme/2014/09/programme/?zsid=8621885e62c6e1a

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Practical Microbiology for Secondary Schools: A Resource for Key Stages 3, 4 and Post-16 and the Equivalent Scottish Qualifications
Fry, P., Grainger, J., Hurst, J., Hoyles, L. and Noble, S. Fry, P., Grainger, J. and Hurst, J. (ed.) 2002. Practical Microbiology for Secondary Schools: A Resource for Key Stages 3, 4 and Post-16 and the Equivalent Scottish Qualifications. Reading Society for General Microbiology.

Actinomyces coleocanis sp. nov., from the vagina of a dog
Hoyles, L., Falsen, E., Foster, G. and Collins M, D. 2002. Actinomyces coleocanis sp. nov., from the vagina of a dog. International Journal of Systematic and Evolutionary Microbiology. 52 (4), pp. 1201-1203. https://doi.org/10.1099/ijs.0.01934-0

Bifidobacterium scardovii sp. nov., from human sources
Hoyles, L., Inganas, E., Falsen, E., Drancourt, M., Weiss, N., McCartney, A.L. and Collins, M.D. 2002. Bifidobacterium scardovii sp. nov., from human sources. International Journal of Systematic and Evolutionary Microbiology. 52 (3), pp. 995-999. https://doi.org/10.1099/ijs.0.02065-0

Arcanobacterium hippocoleae sp. nov., from the vagina of a horse
Hoyles, L., Falsen, E., Foster, G., Rogerson, F. and Collins M, D. 2002. Arcanobacterium hippocoleae sp. nov., from the vagina of a horse. International Journal of Systematic and Evolutionary Microbiology. 52 (2), pp. 617-619. https://doi.org/10.1099/ijs.0.01950-0

Arthrobacter nasiphocae sp. nov., from the common seal (Phoca vitulina)
Collins M, D., Hoyles, L., Foster, G., Falsen, E. and Weiss, N. 2002. Arthrobacter nasiphocae sp. nov., from the common seal (Phoca vitulina). International Journal of Systematic and Evolutionary Microbiology. 52, pp. 569-571. https://doi.org/10.1099/ijs.0.01932-0

Characterization of some strains from human clinical sources which resemble "Leptotrichia sanguinegens": description of Sneathia sanguinegens sp. nov., gen. nov.
Collins M, D., Hoyles, L., Tornqvist, E., von Essen, R. and Falsen, E. 2001. Characterization of some strains from human clinical sources which resemble "Leptotrichia sanguinegens": description of Sneathia sanguinegens sp. nov., gen. nov. Systematic and Applied Microbiology. 24 (3), pp. 358-361. https://doi.org/10.1078/0723-2020-00047

Facklamia miroungae sp. nov., from a juvenile southern elephant seal (Mirounga leonina)
Hoyles, L., Foster, G., Falsen, E., Thomson L, F. and Collins M, D. 2001. Facklamia miroungae sp. nov., from a juvenile southern elephant seal (Mirounga leonina). International Journal of Systematic and Evolutionary Microbiology. 51 (4), pp. 1401-1403. https://doi.org/10.1099/00207713-51-4-1401

Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat
Collins M, D., Hoyles, L., Hutson R, A., Foster, G. and Falsen, E. 2001. Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. International Journal of Systematic and Evolutionary Microbiology. 51 (4), pp. 1349-1352. https://doi.org/10.1099/00207713-51-4-1349

Actinomyces suimastitidis sp. nov., isolated from pig mastitis
Hoyles, L., Falsen, E., Holmstrom, G., Persson, A., Sjoden, B. and Collins, M.D. 2001. Actinomyces suimastitidis sp. nov., isolated from pig mastitis. International Journal of Systematic and Evolutionary Microbiology. 51 (4), pp. 1323-1326. https://doi.org/10.1099/00207713-51-4-1323

Corynebacterium capitovis sp. nov., from a sheep
Collins, M.D., Hoyles, L., Foster, G., Sjoden, B. and Falsen, E. 2001. Corynebacterium capitovis sp. nov., from a sheep. International Journal of Systematic and Evolutionary Microbiology. 51 (3), pp. 857-860. https://doi.org/10.1099/00207713-51-3-857

Streptococcus ovis sp. nov., isolated from sheep
Collins M, D., Hutson R, A., Hoyles, L., Falsen, E., Nikolaitchouk, N. and Foster, G. 2001. Streptococcus ovis sp. nov., isolated from sheep. International Journal of Systematic and Evolutionary Microbiology. 51 (3), pp. 1147-1150. https://doi.org/10.1099/00207713-51-3-1147

Actinomyces catuli sp. nov., from dogs
Hoyles, L., Falsen, E., Pascual, C., Sjoden, B., Foster, G., Henderson, D. and Collins, M.D. 2001. Actinomyces catuli sp. nov., from dogs. International Journal of Systematic and Evolutionary Microbiology. 51 (2), pp. 679-682. https://doi.org/10.1099/00207713-51-2-679

Arcanobacterium/Corynebacterium-like bacterial isolates from sheep
Foster, G., Ritchie, C., Cowie, R.A., Rusbridge, S.M., Collins, M.D. and Hoyles, L. 2001. Arcanobacterium/Corynebacterium-like bacterial isolates from sheep. Veterinary Record. 148 (9), p. 284.

Actinomyces marimammalium sp. nov., from marine mammals
Hoyles, L., Pascual, C., Falsen, E., Foster, G., Grainger, J.M. and Collins, M.D. 2001. Actinomyces marimammalium sp. nov., from marine mammals. International Journal of Systematic and Evolutionary Microbiology. 51 (1), pp. 151-156. https://doi.org/10.1099/00207713-51-1-151

Characterization of a Gemella-like organism isolated from an abscess of a rabbit: description of Gemella cunicula sp. nov.
Hoyles, L., Foster, G., Falsen, E. and Collins M, D. 2000. Characterization of a Gemella-like organism isolated from an abscess of a rabbit: description of Gemella cunicula sp. nov. International Journal of Systematic and Evolutionary Microbiology. 50 (6), pp. 2037-2041. https://doi.org/10.1099/00207713-50-6-2037

Characterization of Actinomyces isolates from infected root canals of teeth: description of Actinomyces radicidentis sp. nov.
Collins M, D., Hoyles, L., Kalfas, S., Sundquist, G., Monsen, T., Nikolaitchouk, N. and Falsen, E. 2000. Characterization of Actinomyces isolates from infected root canals of teeth: description of Actinomyces radicidentis sp. nov. Journal of Clinical Microbiology. 38 (9), pp. 3399-3403.

Characterization of Actinomyces isolates from samples from the human urogenital tract: description of Actinomyces urogenitalis sp. nov.
Nikolaitchouk, N., Hoyles, L., Falsen, E., Grainger J, M. and Collins M, D. 2000. Characterization of Actinomyces isolates from samples from the human urogenital tract: description of Actinomyces urogenitalis sp. nov. International Journal of Systematic and Evolutionary Microbiology. 50 (4), pp. 1649-1654. https://doi.org/10.1099/00207713-50-4-1649

Actinomyces canis sp. nov., isolated from dogs
Hoyles, L., Falsen, E., Foster, G., Pascual, C., Greko, C. and Collins, M.D. 2000. Actinomyces canis sp. nov., isolated from dogs. International Journal of Systematic and Evolutionary Microbiology. 50 (4), pp. 1547-1551. https://doi.org/10.1099/00207713-50-4-1547

Vagococcus fessus sp. nov., isolated from a seal and a harbour porpoise
Hoyles, L., Lawson P, A., Foster, G., Falsen, E., Ohlen, M., Grainger J, M. and Collins M, D. 2000. Vagococcus fessus sp. nov., isolated from a seal and a harbour porpoise. International Journal of Systematic and Evolutionary Microbiology. 50 (3), pp. 1151-1154. https://doi.org/10.1099/00207713-50-3-1151

Phenotypic and phylogenetic characterization of a new Corynebacterium species from dogs: description of Corynebacterium auriscanis sp. nov.
Collins M, D., Hoyles, L., Lawson P, A., Falsen, E., Robson R, L. and Foster, G. 1999. Phenotypic and phylogenetic characterization of a new Corynebacterium species from dogs: description of Corynebacterium auriscanis sp. nov. Journal of Clinical Microbiology. 37 (11), pp. 3443-3447.

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