Title | Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization |
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Authors | Iqbal, H.M.N., Kyazze, G., Tron, T. and Keshavarz, T. |
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Abstract | In the present study, we propose a green route to prepare poly(3-hydroxybutyrate) [(P(3HB)] grafted ethyl cellulose (EC) based green composites with novel characteristics through laccase-assisted grafting. P(3HB) was used as a side chain whereas, EC as a backbone material under an ambient processing conditions. A novel laccase obtained from Aspergillus niger through its heterologous expression in Saccharomyces cerevisiae was used as a green catalyst for grafting purposes without the use of additional initiator and/or cross-linking agents. Subsequently, the resulting P(3HB)-g-EC composites were characterized using a range of analytical and imagining techniques. Fourier transform infrared spectroscopy (FT-IR) spectra showed an increase in the hydrogen-bonding type interactions between the side chains of P(3HB) and backbone material of EC. Evidently, X-ray diffraction (XRD) analysis revealed a decrease in the crystallinity of the P(3HB)-g-EC composites as compared to the pristine individual polymers. A homogeneous P(3HB) distribution was also achieved in case of the graft composite prepared in the presence of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a mediator along with laccase as compared to the composite prepared using pure laccase alone. A substantial improvement in the thermal and mechanical characteristics was observed for grafted composites up to the different extent as compared to the pristine counterparts. The hydrophobic/hydrophilic properties of the grafted composites were better than those of the pristine counterparts. |
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Keywords | Biological polymers; Composite materials; Laccase; Aspergillus niger |
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Journal | Saudi Journal of Biological Sciences |
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Journal citation | 25 (3), pp. 545-550 |
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ISSN | 1319-562X |
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Year | 2018 |
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Publisher | Elsevier |
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Publisher's version | |
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Digital Object Identifier (DOI) | https://doi.org/10.1016/j.sjbs.2016.01.027 |
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Publication dates |
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Published in print | Mar 2018 |
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Published | 21 Jan 2016 |
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License | CC BY-NC-ND 4.0 |
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