Zinc-dependent multimerization of mutant calreticulin is required for MPL binding and MPN pathogenesis

Molecular simulations and virtual screening to investigate the structure and binding sites of the Influenza A virus Nuclear Export Protein
Patel, H. and Kukol, A 2022. Molecular simulations and virtual screening to investigate the structure and binding sites of the Influenza A virus Nuclear Export Protein. Women in Bioinformatics and Data Science. Online 20 - 22 Sep 2022

C2 by-pass: cross-talk between the complement classical and alternative pathways
Laich, A., Patel, H., Zarantonello, A., Sim, R.B. and Inal, J.M. 2022. C2 by-pass: cross-talk between the complement classical and alternative pathways. Immunobiology. 227 (3) 152225. https://doi.org/10.1016/j.imbio.2022.152225

Modelling and Simulation of Proteins
Patel, H. and Kukol, A. 2021. Modelling and Simulation of Proteins. in: Rapley, R. (ed.) Molecular Biology and Biotechnology Royal Society of Chemistry. pp. 394-409

Proteins and Proteomics
Patel, H. and Whitehouse, D. 2021. Proteins and Proteomics. in: Rapley, R. (ed.) Molecular Biology and Biotechnology Royal Society of Chemistry. pp. 123-152

Cohesin mutations alter DNA damage repair and chromatin structure and create therapeutic vulnerabilities in MDS/AML
Tothova, Z., Valton, A., Gorelov, R., Vallurupalli, M., Krill-Burger, J.M., Holmes, A., Landers, C.C., Haydu, J.E., Malolepsza, E., Hartigan, C.R., Donahue, M., Popova, K.D., Koochaki, S.H.J., Venev, S.V., Rivera, J.F., Chen, E., Lage, K., Schenone, M., D'Andrea, A.D., Carr, S.A., Morgan, E.A., Dekker, J. and Ebert, B.L. 2021. Cohesin mutations alter DNA damage repair and chromatin structure and create therapeutic vulnerabilities in MDS/AML. JCI Insight. 6 (3) e142149. https://doi.org/10.1172/jci.insight.142149

Integrating molecular modelling methods to advance influenza A virus drug discovery
Patel, H. and Kukol, A. 2021. Integrating molecular modelling methods to advance influenza A virus drug discovery. Drug Discovery Today. 26 (2), pp. 503-510. https://doi.org/10.1016/j.drudis.2020.11.014

Microbial Proteomics
Patel, H. and Whitehouse, D. 2019. Microbial Proteomics. in: Rapley, R. and Whitehouse, D. (ed.) Genomics and Clinical Diagnostics The Royal Society of Chemistry. pp. 103-139

Prediction of ligands to universally conserved binding sites of the influenza A virus nuclear export protein
Patel, H. and Kukol, A 2019. Prediction of ligands to universally conserved binding sites of the influenza A virus nuclear export protein. Virology. 537, pp. 97-103. https://doi.org/10.1016/j.virol.2019.08.013

Mechanism of completion of peptidyltransferase centre assembly in eukaryotes
Kargas, V., Castro-Hartmann, P., Escudero-Urquijo, N., Dent, K., Hilcenko, C., Sailer, C., Zisser, G., Marques-Carvalho, M.J., Pellegrino, S., Wawiorka, L., Freund, S.M., Wagstaff, J.L., Andreeva, A., Faille, A., Chen, E., Stengel, F., Bergler, H. and Warren, A.J. 2019. Mechanism of completion of peptidyltransferase centre assembly in eukaryotes. eLife. 8, p. e44904 e44904. https://doi.org/10.7554/eLife.44904

Defining the requirements for the pathogenic interaction between mutant calreticulin and MPL in MPN
Elf, S., Abdelfattah, N.S., Baral, A.J., Beeson, D., Rivera, J.F., Ko, A., Florescu, N., Birrane, G., Chen, E. and Mullally, A. 2018. Defining the requirements for the pathogenic interaction between mutant calreticulin and MPL in MPN. Blood. 131 (7), pp. 782-786. https://doi.org/10.1182/blood-2017-08-800896

Evolutionary conservation of influenza A PB2 sequences reveals potential target sites for small molecule inhibitors.
Patel, H. and Kukol, A. 2017. Evolutionary conservation of influenza A PB2 sequences reveals potential target sites for small molecule inhibitors. Virology. 509, pp. 112-120. https://doi.org/10.1016/j.virol.2017.06.009

Recent discoveries of influenza A drug target sites to combat virus replication
Patel, H. and Kukol, A 2016. Recent discoveries of influenza A drug target sites to combat virus replication. Biochemical Society Transactions. 44 (3), pp. 932-936. https://doi.org/10.1042/BST20160002

Evaluation of a novel virtual screening strategy using receptor decoy binding sites
Patel, H. and Kukol, A. 2016. Evaluation of a novel virtual screening strategy using receptor decoy binding sites. Journal of Negative Results in BioMedicine. 15, pp. 1-5. https://doi.org/10.1186/s12952-016-0058-8

STAT1 activation in association with JAK2 exon 12 mutations
Godfrey, A.L., Chen, E., Massie, C.E., Silber, Y., Pagano, F., Bellosillo, B., Guglielmelli, P., Harrison, C.N., Reilly, J.T., Stegelmann, F., Bijou, F., Lippert, E., Boiron, J.M., Dohner, K., Vannucchi, A.M., Besses, C. and Green, A.R. 2016. STAT1 activation in association with JAK2 exon 12 mutations. Haematologica. 101, pp. e15-e19. https://doi.org/10.3324/haematol.2015.128546

Rps14 haploinsufficiency causes a block in erythroid differentiation mediated by S100A8 and S100A9
Schneider, R.K., Schenone, M., Ferreira, M.V., Kramann, R., Joyce, C.E., Hartigan, C., Beier, F., Brümmendorf, T.H., Germing, U., Platzbecker, U., Busche, G., Knuchel, R., Chen, M.C., Waters, C.S., Chen, E., Chu, L.P., Novina, C.D., Lindsley, R.C., Carr, S.A. and Ebert, B.L. 2016. Rps14 haploinsufficiency causes a block in erythroid differentiation mediated by S100A8 and S100A9. Nature Medicine. 22, pp. 288-297. https://doi.org/10.1038/nm.4047

JAK2V617F mediates resistance to DNA damage-induced apoptosis by modulating FOXO3A localization and Bcl-xL deamidation
Ahn, J.S., Li, J., Chen, E., Kent, D.G., Park, H.J. and Green, A.R. 2016. JAK2V617F mediates resistance to DNA damage-induced apoptosis by modulating FOXO3A localization and Bcl-xL deamidation. Oncogene. 35, pp. 2235-2246. https://doi.org/10.1038/onc.2015.285

Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation
Elf, S., Abdelfattah, N.S., Chen, E., Perales-Patón, J., Rosen, E.A., Ko, A., Peskier, F., Florescu, N., Giannini, S., Wolach, O., Morgan, E.A., Tothova, Z., Losman, J.A., Schneider, R.K., Al-Shahrour, F. and Mullally, A. 2016. Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation. Cancer Discovery. 6, pp. 368-381. https://doi.org/10.1158/2159-8290.CD-15-1434

Distinct effects of concomitant Jak2V617F expression and Tet2 loss in mice combine to promote disease progression in myeloproliferative neoplasms
Chen, E., Schneider, R.K., Breyfogle, L.J., Rosen, E.A., Poveromo, L., Elf, S., Ko, A., Brumme, K., Levine, R., Ebert, B.L. and Mullally, A. 2015. Distinct effects of concomitant Jak2V617F expression and Tet2 loss in mice combine to promote disease progression in myeloproliferative neoplasms. Blood. 125, pp. 327-335. https://doi.org/10.1182/blood-2014-04-567024

Genetic variation at MECOM, TERT, JAK2 and HBS1L-MYB predisposes to myeloproliferative neoplasms
Tapper, W., Jones, A.V., Kralovics, R., Harutyunyan, A.S., Zoi, K., Leung, W., Godfrey, A.L., Guglielmelli, P., Callaway, A., Ward, D., Aranaz, P., White, H.E., Waghorn, K., Lin, F., Chase, A., Baxter, E.J., Maclean, C., Nangalia, J., Chen, E., Evans, P., Short, M., Jack, A., Wallis, L., Oscier, D., Duncombe, A.S., Schuh, A., Mead, A.J., Griffiths, M., Ewing, J., Gale, R.E., Schnittger, S., Haferlach, T., Stegelmann, F., Dohner, K., Grallert, H., Strauch, K., Tanaka, T., Bandinelli, S., Giannopoulos, A., Pieri, L., Mannarelli, C., Gisslinger, H., Barosi, G., Cazzola, M., Reiter, A., Harrison, C., Campbell P., Green, A.R., Vannucchi, A. and Cross N.C. 2015. Genetic variation at MECOM, TERT, JAK2 and HBS1L-MYB predisposes to myeloproliferative neoplasms. Nature Communications . 6 6691. https://doi.org/10.1038/ncomms7691

RECQL5 suppresses oncogenic JAK2-induced replication stress and genomic instability
Chen, E., Ahn, J.S., Sykes, D.B., Breyfogle, L.J., Godfrey, A.L., Nangalia, J., Ko, A., DeAngelo, D.J., Green, A.R. and Mullally, A. 2015. RECQL5 suppresses oncogenic JAK2-induced replication stress and genomic instability. Cell Reports. 13, pp. 2345-2532. https://doi.org/10.1016/j.celrep.2015.11.037

Influenza A nucleoprotein binding sites for antivirals: current research and future potential
Patel, H. and Kukol, A 2014. Influenza A nucleoprotein binding sites for antivirals: current research and future potential. Future Virology. 9, pp. 625-627. https://doi.org/10.2217/fvl.14.45

JAK2V617F homozygosity drives a phenotypic switch in myeloproliferative neoplasms, but is insufficient to sustain disease
Li, J., Kent, D.G., Godfrey, A.L., Manning, H., Nangalia, J., Aziz, A., Chen, E., Saeb-Parsy, K., Find, J., Sneade, R., Hamilton, T.L., Pask, D.C., Silber, Y., Zhao, X., Ghevaert, C., Liu, P. and Green, A.R. 2014. JAK2V617F homozygosity drives a phenotypic switch in myeloproliferative neoplasms, but is insufficient to sustain disease. Blood. 123, pp. 3139-3151. https://doi.org/10.1182/blood-2013-06-510222

JAK2V617F promotes replication fork stalling with disease-restricted impairment of the intra-S checkpoint response
Chen, E., Ahn, J.S., Massie, C.E., Clynes, D., Godfrey, A.L., Li, J., Park, H.J., Nangalia, J., Silber, Y., Mullally, A., Gibbons, R.J. and Green, A.R. 2014. JAK2V617F promotes replication fork stalling with disease-restricted impairment of the intra-S checkpoint response. Proceedings of the National Academy of Sciences of the United States of America. 111, pp. 15190-15195. https://doi.org/10.1073/pnas.1401873111

How does JAK2V617F contribute to pathogenesis of myeloproliferative neoplasms? (Review)
Chen, E. and Mullally, A. 2014. How does JAK2V617F contribute to pathogenesis of myeloproliferative neoplasms? (Review). Hematology American Society of Hematology Education Program. 2014, pp. 268-276. https://doi.org/10.1182/asheducation-2014.1.268

Clonal analysis reveal associations of JAK2V617F homozygosity with hematological features, age and gender in PV and ET
Godfrey, A.L., Chen, E., Pagano, F., Silber, Y., Campbell, P.J. and Green, A.R. 2013. Clonal analysis reveal associations of JAK2V617F homozygosity with hematological features, age and gender in PV and ET. Haematologica. 98, pp. 718-721. https://doi.org/10.3324/haematol.2012.079129

JAK2V617F homozygosity arises commonly and recurrently in PV and ET, but PV is characterized by expansion of a dominant homozygous subclone
Godfrey, A.L., Chen, E., Pagano, F., Ortmann, C.A., Silber, Y., Belosillo, B., Guglielmelli, P., Harrison, C., Reilly, J.T., Stegelmann, F., Bijou, F., Lippert, E., McMullin, M.F., Boiron, J.M., Doehner, K., Vannucchi, A.M., Besses, C., Campbell, P.J. and Green, A.R. 2012. JAK2V617F homozygosity arises commonly and recurrently in PV and ET, but PV is characterized by expansion of a dominant homozygous subclone. Blood. 120, pp. 2704-2707. https://doi.org/10.1182/blood-2012-05-431791

Janus kinase deregulation in leukemia and lymphoma (Review)
Chen, E., Staudt, L.M. and Green, A.R. 2012. Janus kinase deregulation in leukemia and lymphoma (Review). Immunity. 36 (4), pp. 529-541. https://doi.org/10.1016/j.immuni.2012.03.017

Mouse models of myeloproliferative Neoplasms: JAK of all grades. (Review)
Li, J., Kent, D.G., Chen, E. and Green, A.R. 2011. Mouse models of myeloproliferative Neoplasms: JAK of all grades. (Review). Disease Models and Mechanisms. 4, pp. 311-317. https://doi.org/10.1242/dmm.006817

Two routes to leukemic transformation following a JAK2 mutation-positive myeloproliferative neoplasm
Beer, P.A., Delhommeau, F., Lecouedic, J.P., Dawson, M.A., Chen, E., Bareford, D., Kusec, R., McMullin, M.F., Harrison, C.N., Vannucchi, A., Vainchenker, W. and Green, A.R. 2010. Two routes to leukemic transformation following a JAK2 mutation-positive myeloproliferative neoplasm. Blood. 115, pp. 2891-2900. https://doi.org/10.1182/blood-2009-08-236596

JAK2 V617F impairs hematopoietic stem cell function in a conditional knock-in mouse model of JAK2 V617F-positive essential thrombocythemia
Li, J., Spensberger, D., Ahn, J.S., Anand, S., Beer, P.A., Ghevaert, C., Chen, E., Forrai, A., Scott, L.M., Ferreira, R., Campbell, P.J., Watson, S.P., Liu, P., Erber, W.N., Huntly, B.J., Ottersbach, K. and Green, A.R. 2010. JAK2 V617F impairs hematopoietic stem cell function in a conditional knock-in mouse model of JAK2 V617F-positive essential thrombocythemia. Blood. 116, pp. 1528-1538. https://doi.org/10.1182/blood-2009-12-259747

Distinct clinical phenotypes associated with JAK2V617F reflect differential STAT1 signaling
Chen, E., Beer, P.A., Godfrey, A.L., Ortmann, C.A., Li, J., Costa-Pereira, A.P., Ingle, C.E., Dermitzakis, E.T., Campbell, P.J. and Green, A.R. 2010. Distinct clinical phenotypes associated with JAK2V617F reflect differential STAT1 signaling. Cancer Cell. 18, pp. 524-535. https://doi.org/10.1016/j.ccr.2010.10.013

Id1 promotes expansion and survival of primary erythroid cells and is a target of JAK2V617F-STAT5 signalling
Wood, A.D., Chen, E., Donaldson, I.J., Hattangadi, S., Burke, K.A., Dawson, M.A., Miranda-Saavendra, D., Lodish, H.F., Green, A.R. and Gottgens, B. 2009. Id1 promotes expansion and survival of primary erythroid cells and is a target of JAK2V617F-STAT5 signalling. Blood. 114, pp. 1820-1830. https://doi.org/10.1182/blood-2009-02-206573

Dysregulated expression of mitotic regulators is associated with B-cell lymphomagenesis in HOX11-Transgenic mice
Chen, E., Lim, M.S., Rosic-Kablar, S., Liu, J., Jolicoeur, P., Dube, I.D. and Hough, M.R. 2006. Dysregulated expression of mitotic regulators is associated with B-cell lymphomagenesis in HOX11-Transgenic mice. Oncogene. 25, pp. 2575-2587. https://doi.org/10.1038/sj.onc.1209285

Loss of UBR1 promotes aneuploidy and accelerates B cell lymphomagenesis in TLX1/HOX11-Transgenic mice
Chen, E., Kwon, Y.T., Lim, M.S., Dube, I.D. and Hough, M.R. 2006. Loss of UBR1 promotes aneuploidy and accelerates B cell lymphomagenesis in TLX1/HOX11-Transgenic mice. Oncogene. 25, pp. 5752-5763. https://doi.org/10.1038/sj.onc.1209573