Title | Peptidylarginine Deiminase Inhibition Abolishes the Production of Large Extracellular Vesicles From |
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Type | Journal article |
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Authors | Gavinho, Bruno, Sabatke, Bruna, Feijoli, Veronica, Rossi, Izadora Volpato, da Silva, Janaina Macedo, Evans-Osses, Ingrid, Palmisano, Giuseppe, Lange, Sigrun and Ramirez, Marcel Ivan |
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Abstract | is a microaerophilic protozoan that is an important etiologic agent of diarrhea worldwide. There is evidence that under diverse conditions, the parasite is capable of shedding extracellular vesicles (EVs) which modulate the physiopathology of giardiasis. Here we describe new features of EV production, revealing its capacity to shed two different enriched EV populations: large (LEV) and small extracellular vesicles (SEV) and identified relevant adhesion functions associated with the larger population. Proteomic analysis revealed differences in proteins relevant for virulence and host-pathogen interactions between the two EV subsets, such as cytoskeletal and anti-oxidative stress response proteins in LEVS. We assessed the effect of two recently identified inhibitors of EV release in mammalian cells, namely peptidylarginine deiminase (PAD) inhibitor and cannabidiol (CBD), on EV release from . The compounds were both able to effectively reduce EV shedding, the PAD-inhibitor specifically affecting the release of LEVs and reducing parasite attachment to host cells . Our results suggest that LEVs and SEVs have a different role in host-pathogen interaction, and that treatment with EV-inhibitors may be a novel treatment strategy for recurrent giardiasis. [Abstract copyright: Copyright © 2020 Gavinho, Sabatke, Feijoli, Rossi, da Silva, Evans-Osses, Palmisano, Lange and Ramirez.] |
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Keywords | Giardia intestinalis |
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| PAD inhibitors |
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| cannabiol |
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| diarrhea |
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| extracellular vesicles (EVs) |
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| large vesicle-like structures |
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| parasite host cell-interaction |
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| small vesicles |
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Journal | Frontiers in Cellular and Infection Microbiology |
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Journal citation | 10, p. 417 |
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ISSN | 2235-2988 |
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Year | 2020 |
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Publisher | Frontiers |
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Publisher's version | License CC BY 4.0 File Access Level Open (open metadata and files) |
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Digital Object Identifier (DOI) | https://doi.org/10.3389/fcimb.2020.00417 |
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PubMed ID | 33072615 |
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Publication dates |
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Published online | 23 Sep 2020 |
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