Ter Heegde, F., Jones, R.C., Jackson, T.R., De Pascale, C., Locke, I.C., Board, T.N., Richardson, S.M., Townsend, P.A. and Lawrence, K.M. 2018. Urocortin expression in cartilage is attenuated in a non-invasive mouse model of post traumatic osteoarthritis. OARSI World Congress on Osteoarthritis . Toronto, Canada 29 2018 - 26 Apr 2019 Elsevier. https://doi.org/10.1016/j.joca.2018.02.206
| Title | Urocortin expression in cartilage is attenuated in a non-invasive mouse model of post traumatic osteoarthritis |
|---|---|
| Authors | Ter Heegde, F., Jones, R.C., Jackson, T.R., De Pascale, C., Locke, I.C., Board, T.N., Richardson, S.M., Townsend, P.A. and Lawrence, K.M. |
| Type | Conference poster |
| Abstract | Purpose: Traumatic injury to the joint commonly develops into an accelerated form of osteoarthritis. This post traumatic osteoarthritis (PTO) is frequently associated with sports injury and represents a significant proportion of patients presenting with osteoarthritis. Currently, there are no treatments other than analgesics and joint replacement surgery. The development of PTO, although multifactorial, is closely associated with chondrocyte cell death. We have recently found that the peptide Urocortin (Ucn1), its receptor (CRF-R1) and regulatory binding protein (CRF-BP) are expressed by chondrocytes, thus constituting an intact and functional Ucn1 signalling system. Furthermore, in a cartilage explant model of acute impact injury, we found that Ucn1 potently protects chondrocytes from death when applied both pre- and post-impact. These finding suggests a potential role for Ucn1 in preventing cartilage tissue damage in PTO and as a novel therapeutic. Studies investigating the effects of mechanical loading on articular cartilage have been performed in vitro or with ex vivo cartilage explants. However, these are not ideal as crucial interactions between cells and their environment are lost, and reliable pathology may be compromised by dissection and culture. The most common in vivo models that simulate mechanical injury involve surgical joint destabilisation; these are severely disadvantaged by the need for surgery, increasing infection risk, trauma, and effects on joint cell metabolism. There is, therefore, a need for a nonsurgical model allowing for adjustment of specific components of the applied load, including its magnitude, frequency,and loading cycle shape, which can reflect the variable loads encountered throughout life and importantly display a developmental pathology similar to PTO in humans. Methods: Here we used a non-invasive controlled axial loading of the mouse tibia model, enabling loading through normal points of articulation and precisely controlled mechanical loading regimens. In order to induce osteoarthritis, the right knee of 12 week old male C57/bl6 mice was loaded at 9N (pathological load). A single loading episode consisted Results: It has been previously demonstrated that the loading regimen presented in Schematic1 causes significant development of PTO when compared to a holding load alone. We found positive immunostaining in cartilage tissue with all three antibodies for the Ucn system members. Expression levels of CRF-R1 and CRF-BP did not alter with load severity. However, we found that expression of Ucn1 itself was markedly reduced in the cartilage of mice exposed to a 9N pathological load Conclusions: We have found expression of Ucn system members in articular cartilage at the protein level in vivo, including the ligand itself, its receptor and regulatory binding protein, suggesting an intact signalling pathway and regulatory system.There was a profound decrease in Ucn1 protein expression in the cartilage of joints exposed to a loading regimen which develops into PTO. This finding suggests a post-injury loss of Ucn1 signalling, caused by a decrease in Ucn1 expression, which may be responsible for the initiation or progression of PTO. |
| Year | 2018 |
| Conference | OARSI World Congress on Osteoarthritis |
| Publisher | Elsevier |
| Publication dates | |
| Published | Apr 2018 |
| Journal | Osteoarthritis & Cartilage |
| Journal citation | 26 (1), p. S96 |
| Digital Object Identifier (DOI) | https://doi.org/10.1016/j.joca.2018.02.206 |
Regulation of TNF-Induced Osteoclast Differentiation.
Yao, Zhenqiang, Getting, Stephen J and Locke, Ian C 2021. Regulation of TNF-Induced Osteoclast Differentiation. Cells. 11 (1) e132. https://doi.org/cells11010132
The effect of melanocortin peptides on SNAP activated chondrocytes
Can, V.C., Locke, I.C., Grieco, P. and Getting, S.J. 2020. The effect of melanocortin peptides on SNAP activated chondrocytes. 2020 OARSI World Congress on Osteoarthritis. 30 Apr - 03 May 2020 Elsevier. https://doi.org/10.1016/j.joca.2020.02.203
Novel anti-inflammatory and chondroprotective effects of the human melanocortin MC1 receptor agonist BMS-470539 dihydrochloride and human melanocortin MC3 receptor agonist PG-990 on lipopolysaccharide activated chondrocytes
Can, V., Locke, I.C., Kaneva, M., Kerrigan, M.J.P., Merlino, F., De Pascale, C., Grieco, P. and Getting, S.J. 2020. Novel anti-inflammatory and chondroprotective effects of the human melanocortin MC1 receptor agonist BMS-470539 dihydrochloride and human melanocortin MC3 receptor agonist PG-990 on lipopolysaccharide activated chondrocytes. European Journal of Pharmacology. 872 172971. https://doi.org/10.1016/j.ejphar.2020.172971
Populated Collagen Hydrogel and Polyhydroxyalkanoate Composites: Novel Matrices for Cartilage Repair and Regeneration?
De Pascale, C., Marcello, E., Getting, S.J., Roy, I. and Locke, I.C. Forthcoming. Populated Collagen Hydrogel and Polyhydroxyalkanoate Composites: Novel Matrices for Cartilage Repair and Regeneration? OARSI World Congress on Osteoarthritis . Toronto, Canada 29 2018 - 26 Apr 2019
Effect of Urocortin on human osteoclasts differentiation
De Pascale, C., Getting, S.J. and Locke, I.C. 2018. Effect of Urocortin on human osteoclasts differentiation. British Pharmacological Society Meeting. the Queen Elizabeth II Conference Centre 18 - 20 Dec 2018 British Pharmacological Society.
Poly(3-hydroxyoctanoate), a promising new material for cardiac tissue engineering
Bagdadi, A., Safari, M., Dubey, P., Basnett, P., Sofokleous P., Humphrey E, Locke, I.C., Edirisinghe M., Terracciano C., Boccaccini, A.R., Knowles, J.C., Harding, S. and Roy, I. 2018. Poly(3-hydroxyoctanoate), a promising new material for cardiac tissue engineering. Journal of Tissue Engineering and Regenerative Medicine. 12 (1), pp. E495-E512. https://doi.org/10.1002/term.2318
The effect of the selective MC3 agonist PG990 on high density human chondrocytes micromass cultures activated by TNF-alpha
Can, V.C., Grieco, P, Locke, I.C. and Getting, S.J. 2017. The effect of the selective MC3 agonist PG990 on high density human chondrocytes micromass cultures activated by TNF-alpha. World Congress on Inflammation. London 08 - 12 Jul 2017
The effect of the selective human MC3 receptor agonist PG992 on high density human chondrocyte micromass cultures activated by IL-1beta.
Can, V.C., Locke, I.C., Grieco, P. and Getting, S.J. 2017. The effect of the selective human MC3 receptor agonist PG992 on high density human chondrocyte micromass cultures activated by IL-1beta. Pharmacology 2017. London, U.K. 11 - 13 Dec 2017 British Pharmacological Society.
The anti-inflammatory effect of silymarin in chondrocytes
Can, V.C., Abouelnour, A., Locke, I.C., Bligh, S.W.A. and Getting, S.J. 2017. The anti-inflammatory effect of silymarin in chondrocytes. ICMAN/ IUPHAR Natural Products. Aberdeen, Scotland 27 - 29 Sep 2017 Elsevier. https://doi.org/10.1016/j.bcp.2017.06.036
Chondroprotection by urocortin involves blockade of a mechanosensitive piezo1 ion channel: novel, exploitable pathways for the treatment and prevention of osteoarthritis?
Lawrence, K.M., Jones, R.C., Jackson, T.R., Baylie, R.L., Abbot, B., Bruhn-Olszewska, B, Board, T.N., Locke, I.C., Richardson, S.M and Townsend, P.A. 2017. Chondroprotection by urocortin involves blockade of a mechanosensitive piezo1 ion channel: novel, exploitable pathways for the treatment and prevention of osteoarthritis? OARSI 2017 World Congress on Osteoarthritis. Las Vegas, USA. 17 - 20 Apr 2017 Elsevier. https://doi.org/10.1016/j.joca.2017.02.124
Urocortin protects chondrocytes from acute trauma in articular cartilage
Jones, R.C., Jackson, TR, Locke, I.C., Board, T.N., Richardson, S.M., Townsend, P.A. and Lawrence, K.M. 2017. Urocortin protects chondrocytes from acute trauma in articular cartilage. OARSI 2017 World Congress on Osteoarthritis. Las Vegas, USA 27 - 30 Apr 2017 Elsevier. https://doi.org/10.1016/j.joca.2017.02.291
Chondroprotection by urocortin involves blockade of the mechanosensitive ion channel Piezo1
Lawrence, K.M., Jones, R.C., Jackson, T.R., Baylie, R.L., Abbott, B., Bruhn-Olszewska, B., Board, T.N., Locke, I.C., Richardson, S.M. and Townsend, P.A. 2017. Chondroprotection by urocortin involves blockade of the mechanosensitive ion channel Piezo1. Scientific Reports. 7 (1), p. 5147 5147. https://doi.org/10.1038/s41598-017-04367-4
Highly elastomeric poly(3-hydroxyoctanoate) based natural polymer composite for enhanced keratinocyte regeneration
Rai, R., Roether, J.A., Knowles, J.C., Mordan, N., Salih, V., Locke, I.C., Gordge, M.P., McCormick, A., Mohn, D., Stark, W.J., Keshavarz, T., Boccaccini, A.R. and Roy, I. 2017. Highly elastomeric poly(3-hydroxyoctanoate) based natural polymer composite for enhanced keratinocyte regeneration. International Journal of Polymeric Materials and Polymeric Biomaterials . 66 (7), pp. 326-335. https://doi.org/10.1080/00914037.2016.1217530
Effects of the selective MC3 agonists PG990 and PG992 on Interleukin 1beta induced chondrocyte death and inflammation
Can, V.C., Locke, I.C., Grieco P and Getting, S.J. 2016. Effects of the selective MC3 agonists PG990 and PG992 on Interleukin 1beta induced chondrocyte death and inflammation. British Pharmacological Society: Pharmacology 2016. London 13 - 15 Dec 2016 British Pharmacological Society.
Novel P(3HB) Composite Films Containing Bioactive Glass Nanoparticles for Wound Healing Applications
Francis, L., Meng, D., Locke, I.C., Knowles, J.C., Mordan, N., Salih, V., Boccaccini, A.R. and Roy, I. 2016. Novel P(3HB) Composite Films Containing Bioactive Glass Nanoparticles for Wound Healing Applications. Polymer International. 65 (6), pp. 661-674. https://doi.org/10.1002/pi.5108
The effect of melanocortin peptides on Interleukin 1-beta induced chondrocyte cell death and inflammation
Can, V.C., Locke, I.C. and Getting, S.J. 2015. The effect of melanocortin peptides on Interleukin 1-beta induced chondrocyte cell death and inflammation. Physiology 2015 . Cardiff, UK. 08 Jul 2015
Urocortin – From Parkinson’s disease to the skeleton
Lawrence, K.M., Jackson, T.R, Jamieson, D., Stevens, A., Owens, G., Sayan, B.S, Locke, I.C. and Townsend, P.A. 2015. Urocortin – From Parkinson’s disease to the skeleton. International Journal of Biochemistry & Cell Biology. 30, pp. 130-138. https://doi.org/10.1016/j.biocel.2014.12.005
Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application
Iqbal, H.M.N., Kyazze, G., Locke, I.C., Tron, T. and Keshavarz, T. 2015. Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application. International Journal of Biological Macromolecules. 81, pp. 552-559. https://doi.org/10.1016/j.ijbiomac.2015.08.040
Development of novel antibacterial active, HaCaT biocompatible and biodegradable CA-g-P(3HB)-EC biocomposites with caffeic acid as a functional entity
Iqbal, H.M.N., Kyazze, G., Locke, I.C., Tron, T. and Keshavarz, T. 2015. Development of novel antibacterial active, HaCaT biocompatible and biodegradable CA-g-P(3HB)-EC biocomposites with caffeic acid as a functional entity. eXPRESS Polymer Letters. 9 (9), p. 764–772. https://doi.org/10.3144/expresspolymlett.2015.72
Development of bio-composites with novel characteristics: Evaluation of phenol-induced antibacterial, biocompatible and biodegradable behaviours
Iqbal, H.M.N., Kyazze, G., Locke, I.C., Tron, T. and Keshavarz, T. 2015. Development of bio-composites with novel characteristics: Evaluation of phenol-induced antibacterial, biocompatible and biodegradable behaviours. Carbohydrate Polymers. 131, pp. 197-207. https://doi.org/10.1016/j.carbpol.2015.05.046
In-situ development of self-defensive antibacterial biomaterials: phenol-g-keratin-EC based bio-composites with characteristics for biomedical applications
Iqbal, H.M.N., Kyazze, G., Locke, I.C., Tron, T. and Keshavarz, T. 2015. In-situ development of self-defensive antibacterial biomaterials: phenol-g-keratin-EC based bio-composites with characteristics for biomedical applications. Green Chemistry. 17 (7), pp. 3858-3869. https://doi.org/10.1039/C5GC00715A
The chondroprotective effects of Urocortin are mediated via CRHR1, but not CRHR2, receptors.
Lawrence, K.M., White, O.B., Getting, S.J., Knight, R.A., Locke, I.C. and Townsend, P.A. 2014. The chondroprotective effects of Urocortin are mediated via CRHR1, but not CRHR2, receptors. 9th European Workshop on Cell Death. Paphos, Cyprus 30 Mar - 04 Apr 2014
The chondroprotective agent Urocortin regulates key microRNAs involved in chrondrocyte function and survival
Lawrence KM, Knight RA, Townsend PA and Locke, I.C. 2014. The chondroprotective agent Urocortin regulates key microRNAs involved in chrondrocyte function and survival. 9th European Workshop on Cell Death. Paphos, Cyprus 30 Mar - 04 Apr 2014
Anatomy, embryology, and cell families
Locke, I.C. and Murray, J.F. 2014. Anatomy, embryology, and cell families. in: Orchard, G. and Nation, B. (ed.) Cell Structure & Function Oxford, UK Oxford University Press. pp. 82-110
The melanocortin receptor antagonist SHU9119 inhibits the anti-inflammatory/pro-resolving properties of a-MSH and D[TRP8]-y-MSH in lipopolysaccharide stimulated chondrocytes
Can, V.C., Locke, I.C. and Getting, S.J. 2014. The melanocortin receptor antagonist SHU9119 inhibits the anti-inflammatory/pro-resolving properties of a-MSH and D[TRP8]-y-MSH in lipopolysaccharide stimulated chondrocytes. Pharmacology 2014. Queen Elizabeth Centre II, London, UK 16 Dec 2014 British Pharmacological Society.
Melanocortin peptides protect chondrocytes from mechanically induced cartilage injury
Kaneva, M., Kerrigan, M.J.P., Grieco, P., Curley, G.P., Locke, I.C. and Getting, S.J. 2014. Melanocortin peptides protect chondrocytes from mechanically induced cartilage injury. Biochemical Pharmacology. 92 (2), pp. 336-347. https://doi.org/10.1016/j.bcp.2014.08.019
Neuroprotective efficacy of the endogenous neuropeptide Urocortin in a oxygen-glucose deprivation model of transient cerebral ischaemia with reperfusion
Ashioti, M., Getting, S.J., Locke, I.C. and Milton, N.G.N. 2013. Neuroprotective efficacy of the endogenous neuropeptide Urocortin in a oxygen-glucose deprivation model of transient cerebral ischaemia with reperfusion. British Neuroscience Association 22nd Biennial Meeting. London 7th-10th April 2013
Urocortin protects chondrocytes from NO-induced apoptosis: a future therapy for osteoarthritis?
Intekhab Alam, N.Y., White, O.B., Getting, S.J., Petsa, A., Knight, R.A., Townsend, P.A., Lawrence, K.M. and Locke, I.C. 2013. Urocortin protects chondrocytes from NO-induced apoptosis: a future therapy for osteoarthritis? Cell Death and Disease. 4, p. e717. https://doi.org/10.1038/cddis.2013.231
Aspirin-loaded P(3HO)/P(3HB) blend films: potential materials for biodegradable drug-eluting stents
Basnett, P., Ching, K.Y., Stolz, M., Knowles, J.C., Boccaccini, A.R., Smith, C.L., Locke, I.C. and Roy, I. 2013. Aspirin-loaded P(3HO)/P(3HB) blend films: potential materials for biodegradable drug-eluting stents. Bioinspired, Biomimetic and Nanobiomaterials. 2 (3), pp. 141-153. https://doi.org/10.1680/bbn.13.00009
Novel Poly(3-hydroxyoctanoate)/Poly(3-hydroxybutyrate) blends for medical applications
Basnett, P., Ching, K.Y., Stolz, M., Knowles, J.C., Boccaccini, A.R., Smith, C.L., Locke, I.C., Keshavarz, T. and Roy, I. 2013. Novel Poly(3-hydroxyoctanoate)/Poly(3-hydroxybutyrate) blends for medical applications. Reactive and Functional Polymers. 73 (10), pp. 1340-1348. https://doi.org/10.1016/j.reactfunctpolym.2013.03.019
Urocortin is a novel regulator of osteoclast differentiation and function through inhibition of a canonical transient receptor potential 1-like cation channel
Combs, C., Fuller, K., Kumar, H., Albert, A.P., Pirianov, G., McCormick, J., Locke, I.C., Chambers, T.J. and Lawrence, K.M. 2012. Urocortin is a novel regulator of osteoclast differentiation and function through inhibition of a canonical transient receptor potential 1-like cation channel. Journal of Endocrinology. 212 (2), pp. 187-197. https://doi.org/10.1530/JOE-11-0254
The anti-inflammatory effects of melanocortin peptides in lipopolysaccharide activated chondrocytes
Can, V.C., Kaneva, M., Kerrigan, M.J.P., Locke, I.C. and Getting, S.J. 2012. The anti-inflammatory effects of melanocortin peptides in lipopolysaccharide activated chondrocytes. British Phamacological Society Winter Meeting 2012. London, UK 18th-20th December
Chondroprotective and anti-inflammatory role of melanocortin peptides in TNF-α activated human C-20/A4 chondrocytes
Kaneva, M., Kerrigan, M.J.P., Grieco, P., Curley, G.P., Locke, I.C. and Getting, S.J. 2012. Chondroprotective and anti-inflammatory role of melanocortin peptides in TNF-α activated human C-20/A4 chondrocytes. British Journal of Pharmacology. 167 (1), pp. 67-79. https://doi.org/10.1111/j.1476-5381.2012.01968.x
Chondrocyte CRF receptor expression and Urocortin I mediated chondroprotection
White, O.B., Intekhab-Alam, N.Y., Chowdrey, H.S., Knight, R.A., Locke, I.C. and Intekhab Alam, N.Y. 2011. Chondrocyte CRF receptor expression and Urocortin I mediated chondroprotection. Rheumatology 2011. Brighton, UK 12th-14th April 2011
α-MSH and [DTRP]8-γ-MSH inhibit pro-inflammatory cytokine release from stimulated primary bovine chondrocytes
Kaneva, M., Locke, I.C., Kerrigan, M.J.P. and Getting, S.J. 2011. α-MSH and [DTRP]8-γ-MSH inhibit pro-inflammatory cytokine release from stimulated primary bovine chondrocytes. 10th World Congress on Inflammation. Paris, France 25–29 June 2011
MC1 and MC3 activation inhibits caspase-3 production and activity, promotes cell-viability, and induces IL-10 and HO-1 release from human C20/A4 chondrocytes
Kaneva, M., Kerrigan, M.J.P., Locke, I.C. and Getting, S.J. 2011. MC1 and MC3 activation inhibits caspase-3 production and activity, promotes cell-viability, and induces IL-10 and HO-1 release from human C20/A4 chondrocytes. Physiological Society Meeting 23. University of Oxford
The homopolymer poly(3-hydroxyoctanoate) as a matrix material for soft tissue engineering
Rai, R., Boccaccini, A.R., Knowles, J.C., Mordon, N., Salih, V., Locke, I.C., Moshrefi-Torbati, M., Keshavarz, T. and Roy, I. 2011. The homopolymer poly(3-hydroxyoctanoate) as a matrix material for soft tissue engineering. Journal of Applied Polymer Science. 122 (6), pp. 3606-3617. https://doi.org/10.1002/app.34772
Mechanisms of Urocortin family peptide mediated chondroprotection
White, O.B., Intekhab Alam, N.Y., Chowdrey, H.S., Knight, R.A. and Locke, I.C. 2010. Mechanisms of Urocortin family peptide mediated chondroprotection. OARSI 2010 World Congress on Osteoarthritis. Brussels, Belgium September 23-26, 2010 https://doi.org/10.1016/S1063-4584(10)60288-9
The effect of indentor shape on the creep curves of collagen gel
Locke, I.C., White, O.B., Trwoga, P. and Afoke, A. 2010. The effect of indentor shape on the creep curves of collagen gel. OARSI 2010 World Congress on Osteoarthritis. Brussels, Belgium September 23-26, 2010 https://doi.org/10.1016/S1063-4584(10)60511-0
α-MSH and dTRP8-γ-MSH inhibit TNF-α induced MMP 1 and 13 expression in human C20/A4 chondrocytes
Kaneva, M., Locke, I.C., Kerrigan, M.J.P. and Getting, S.J. 2010. α-MSH and dTRP8-γ-MSH inhibit TNF-α induced MMP 1 and 13 expression in human C20/A4 chondrocytes. British Pharmacological Society Winter Meeting 2010. London, UK 14th-16th December 2010
Melanocortin peptides modulate pro-inflammatory mediator release from TNF-a stimulated C-20/A4 cells
Kaneva, M., Kerrigan, M.J.P., Locke, I.C. and Getting, S.J. 2010. Melanocortin peptides modulate pro-inflammatory mediator release from TNF-a stimulated C-20/A4 cells. British Orthopaedic Research Society Annual Meeting. Newcastle upon Tyne 22 - 23 Jun 2009 Wiley. pp. PO136
The MC3 agonist dTRP8-γ-MSH inhibits IL-6 and IL-8 release from TNF-α stimulated C-20/A4 chondrocytes, an effect blocked by the MC3R antagonist SHU9119
Kaneva, M., Kerrigan, M.J.P., Locke, I.C. and Getting, S.J. 2010. The MC3 agonist dTRP8-γ-MSH inhibits IL-6 and IL-8 release from TNF-α stimulated C-20/A4 chondrocytes, an effect blocked by the MC3R antagonist SHU9119. Physiology 2010. University of Manchester 30 June - 2 July 2010
Fabrication of a novel poly(3-hydroxyoctanoate) / nanoscale bioactive glass composite film with potential as a multifunctional wound dressing
Rai, R., Boccaccini, A.R., Knowles, J.C., Locke, I.C., Gordge, M.P., McCormick, A., Salih, V., Mordon, N., Keshavarz, T. and Roy, I. 2010. Fabrication of a novel poly(3-hydroxyoctanoate) / nanoscale bioactive glass composite film with potential as a multifunctional wound dressing. 5th international conference on times of polymers (TOP) and composites. Ischia, Italy 20–23 June 2010 https://doi.org/10.1063/1.3455552
The influence of tetracycline loading on the surface morphology and biocompatibility of films made from P(3HB) microspheres
Francis, L., Meng, D., Locke, I.C., Mordon, N., Salih, V., Knowles, J.C., Boccaccini, A.R. and Roy, I. 2010. The influence of tetracycline loading on the surface morphology and biocompatibility of films made from P(3HB) microspheres. Advanced Engineering Materials. 12 (7), pp. B260-B268. https://doi.org/10.1002/adem.201080020
Pro-inflammatory mediator release from TNF-α stimulated C-20/A4 cells results in Col II degradation
Kaneva, M., Kerrigan, M.J.P., Locke, I.C. and Getting, S.J. 2009. Pro-inflammatory mediator release from TNF-α stimulated C-20/A4 cells results in Col II degradation. British Orthopaedic Research Society (BORS) Annual Meeting. Newcastle 22nd - 23rd June 2009
Melanocortin peptides modulate pro-inflammatory mediator release from TNF-a stimulated C-20/A4 cells
Kaneva, M., Kerrigan, M.J.P., Locke, I.C. and Getting, S.J. 2009. Melanocortin peptides modulate pro-inflammatory mediator release from TNF-a stimulated C-20/A4 cells. pA2 Online. From the Queen Elizabeth II Conference Centre London, Winter 2009 Meeting: Proceedings of the British Pharmacological Society.
Melanocortin peptide therapy for the treatment of arthritic pathologies
Getting, S.J., Kaneva, M., Bhadresa, Y., Renshaw, D., Leoni, G., Patel, H.B., Kerrigan, M.J.P. and Locke, I.C. 2009. Melanocortin peptide therapy for the treatment of arthritic pathologies. The Scientific World Journal. 9, pp. 1394-1414. https://doi.org/10.1100/tsw.2009.163
Interactions between cell surface protein disulphide isomerase and S-nitrosoglutathione during nitric oxide delivery
Shah, C.M., Bell, S.E., Locke, I.C., Chowdrey, H.S. and Gordge, M.P. 2007. Interactions between cell surface protein disulphide isomerase and S-nitrosoglutathione during nitric oxide delivery. Nitric Oxide. 16 (1), pp. 135-142. https://doi.org/10.1016/j.niox.2006.08.001
The corticotrophin-releasing factor-like peptide urocortin reverses key deficits in two rodent models of Parkinson's disease
Abuirmeileh, A., Lever, R., Kingsbury, A.E., Lees, A.J., Locke, I.C., Knight, R.A., Chowdrey, H.S., Biggs, C.S. and Whitton, P.S. 2007. The corticotrophin-releasing factor-like peptide urocortin reverses key deficits in two rodent models of Parkinson's disease. European Journal of Neuroscience. 26 (2), pp. 417-423. https://doi.org/10.1111/j.1460-9568.2007.05653.x
Biological activity of industrial wood and bark waste from Pinus nigra
Curras Lino, M., Radman, R., Bertaud, F., Nisula, L., Lenon, G., Holbom, B., Locke, I.C., Thompson, S. and Keshavarz, T. 2006. Biological activity of industrial wood and bark waste from Pinus nigra. in: Actual problems of creation of new medicinal preparations of natural origin: the 10th international congress PHYTOPHARM 2006, St.-Petersburg, June 27-30, 2006: proceedings of congress St Petersburg, Russia PHYTOPHARM. pp. 401-403
Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells
Prashar, A., Locke, I.C. and Evans, C.S. 2006. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Proliferation. 39 (4), pp. 241-248. https://doi.org/10.1111/j.1365-2184.2006.00384.x
Interactions between cell surface protein disulphide isomerase and S-nitrosoglutathione during nitric oxide delivery into MEG-01 megakaryocytes
Shah, C.M., Walters, S.E., Locke, I.C., Chowdrey, H.S. and Gordge, M.P. 2005. Interactions between cell surface protein disulphide isomerase and S-nitrosoglutathione during nitric oxide delivery into MEG-01 megakaryocytes. Ireland-UK Platelet Conference 2005. Dublin, Ireland 04-06 Sep 2005
Evidence for a neuroprotective role of the CRH-like peptide, urocortin, in the 6-hydroxydopamine treated rat
Biggs, C.S., Locke, I.C., Knight, R.A., Chowdrey, H.S. and Whitton, P.S. 2005. Evidence for a neuroprotective role of the CRH-like peptide, urocortin, in the 6-hydroxydopamine treated rat. 18th National Meeting of the British Neuroscience Association. Brighton, UK 03-06 Apr 2005
Urocortin attenuates key indicators of nigrostriatal pathway destruction in a rat hemiparkinsonian model
Biggs, C.S., Abuirmeileh, A., Locke, I.C., Knight, R.A., Chowdrey, H.S. and Whitton, P.S. 2005. Urocortin attenuates key indicators of nigrostriatal pathway destruction in a rat hemiparkinsonian model. Proceedings of the British Pharmacological Society. 3 (4).
Cell surface thiol modification by GSNO: a mechanism of nitric oxide delivery?
Shah, C.M., Locke, I.C., Chowdrey, H.S. and Gordge, M.P. 2004. Cell surface thiol modification by GSNO: a mechanism of nitric oxide delivery? 5th UK Nitric Oxide Forum. Edinburgh, UK 12/2004
Protein disulphide isomerase is involved in the delivery of NO from S-nitrosoglutathione into megakaryocytes
Shah, C.M., Locke, I.C., Chowdrey, H.S. and Gordge, M.P. 2004. Protein disulphide isomerase is involved in the delivery of NO from S-nitrosoglutathione into megakaryocytes. 3rd International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide: 4th Anuual Scientific Meeting of the Nitric Oxide Society of Japan. Nara, Japan 24-28 May 2004 https://doi.org/10.1016/j.niox.2004.07.004
Protein disulphide isomerase is involved in the delivery of NO from S-nitrosoglutathione into megakaryocytes
Shah, C.M., Locke, I.C., Chowdrey, H.S. and Gordge, M.P. 2004. Protein disulphide isomerase is involved in the delivery of NO from S-nitrosoglutathione into megakaryocytes. Japan-UK Platelet Conference. Oxford, UK September 2004
Establishment and analysis of 3-D collagen cultures of C-20/A4 chondrocytes
Petsa, A., Chowdrey, H.S., Knight, R.A., Afoke, A. and Locke, I.C. 2004. Establishment and analysis of 3-D collagen cultures of C-20/A4 chondrocytes. OARSI 2004 World Congress on Osteoarthritis. Chicago USA 12/2004 https://doi.org/10.1016/j.joca.2004.09.004
A CRH family peptide protects a human chondrocyte cell line against apoptosis
Intekhab-Alam, N.Y., Chowdrey, H.S., Knight, R.A., Locke, I.C. and Intekhab Alam, N.Y. 2004. A CRH family peptide protects a human chondrocyte cell line against apoptosis. OARSI 2004 World Congress on Osteoarthritis. Chicago USA 12/2004 https://doi.org/10.1016/j.joca.2004.09.004
Cytotoxicity of lavender oil and its major components to human skin cells
Prashar, A., Locke, I.C. and Evans, C.S. 2004. Cytotoxicity of lavender oil and its major components to human skin cells. Cell Proliferation. 37 (3), pp. 221-229. https://doi.org/10.1111/j.1365-2184.2004.00307.x
Functional characteristics of the streptococcal deoxyribonuclease 'streptodornase', a protein with DNase activity present in the medicament Varidase
Locke, I.C. and Carpenter, B.G. 2004. Functional characteristics of the streptococcal deoxyribonuclease 'streptodornase', a protein with DNase activity present in the medicament Varidase. Enzyme and Microbial Technology. 35 (1), pp. 67-73. https://doi.org/10.1016/j.enzmictec.2004.03.009
Rapid S-nitrosothiol metabolism by platelets and megakaryocytes
Shah, C.M., Locke, I.C., Chowdrey, H.S. and Gordge, M.P. 2003. Rapid S-nitrosothiol metabolism by platelets and megakaryocytes. Biochemical Society Transactions. 31 (6), pp. 1450-1452.
Role of protein disulphide isomerase in NO delivery from S-nitrosothiols into megakaryocytes
Shah, C.M., Locke, I.C., Chowdrey, H.S. and Gordge, M.P. 2003. Role of protein disulphide isomerase in NO delivery from S-nitrosothiols into megakaryocytes. 4th UK Nitric Oxide Forum. University of Nottingham, UK December 2003
The behaviour of C-20/A4 chondrocytes in 3-D collagen gel cultures
Petsa, A., Afoke, A., Chowdrey, H.S., Knight, R.A., Harris, A. and Locke, I.C. 2003. The behaviour of C-20/A4 chondrocytes in 3-D collagen gel cultures. OARSI 2003 World Congress on Osteoarthritis. Berlin, Germany October 2003 https://doi.org/10.1016/S1063-4584(03)00192-4
Ion-dependency of the streptococcal deoxyribonuclease "streptodornase", an active constituent of the medicament Varidase
Locke, I.C. and Carpenter, B.G. 2002. Ion-dependency of the streptococcal deoxyribonuclease "streptodornase", an active constituent of the medicament Varidase. Enzyme and Microbial Technology. 31 (4), pp. 482-489. https://doi.org/10.1016/S0141-0229(02)00125-4
Effect of essential oils and their components on cell
Prashar, A., Veness, R., Locke, I.C. and Evans, C.S. 2002. Effect of essential oils and their components on cell. Future Trends in Phytochemistry. Gargnano, Italy
Varidase: the science behind the medicament
Rutter, P.M., Carpenter, B.G., Hill, S.S. and Locke, I.C. 2000. Varidase: the science behind the medicament. Journal of Wound Care. 9 (5), pp. 223-226.
A comparison of the characteristics of circulating anti-myeloperoxidase antibodies in vasculitis with those in non-vasculitic conditions
Locke, I.C., Leaker, B. and Cambridge, G. 1999. A comparison of the characteristics of circulating anti-myeloperoxidase antibodies in vasculitis with those in non-vasculitic conditions. Clinical and Experimental Immunology. 115 (2), pp. 369-376. https://doi.org/10.1046/j.1365-2249.1999.00809.x
A comparison of the variable region genes of two human monoclonal antibodies to myeloperoxidase
Locke, I.C., Rahman, M.A.A. and Cambridge, G. 1998. A comparison of the variable region genes of two human monoclonal antibodies to myeloperoxidase. British Society for Immunology 6th Annual Meeting. Harrogate, UK 12/1998
Adjacent proline residues in the inhibitory domain of the Oct-2 transcription factor play distinct functional roles
Liu, Y.Z., Lee, I.K., Locke, I.C., Dawson, S. and Latchman, D. 1998. Adjacent proline residues in the inhibitory domain of the Oct-2 transcription factor play distinct functional roles. Nucleic Acids Research. 26 (10), pp. 2464-2472. https://doi.org/10.1093/nar/26.10.2464
Purification of a streptococcal deoxyribonuclease by affinity chromatography based on a DNA-cellulose matrix
Locke, I.C., Cox, S.F. and Carpenter, B.G. 1997. Purification of a streptococcal deoxyribonuclease by affinity chromatography based on a DNA-cellulose matrix. Journal of Chromatography A. 788 (1-2), pp. 75-80. https://doi.org/10.1016/S0021-9673(97)00695-X
Autoantibodies to myeloperoxidase in systemic sclerosis
Locke, I.C., Worral, J.G., Leaker, B., Black, C.M. and Cambridge, G. 1997. Autoantibodies to myeloperoxidase in systemic sclerosis. Journal of Rheumatology. 24 (1), pp. 86-89.
Characteristics of antibodies to myeloperoxidase in vasculitic and non-vasculitic conditions
Locke, I.C., Leaker, B. and Cambridge, G. 1996. Characteristics of antibodies to myeloperoxidase in vasculitic and non-vasculitic conditions. American College of Rheumatology 60th National Scientific Meeting. Orlando, USA 10/1996
Characteristics of anti-myeloperoxidase autoantibodies in vasculitis and other conditions
Locke, I.C., Leaker, B. and Cambridge, G. 1996. Characteristics of anti-myeloperoxidase autoantibodies in vasculitis and other conditions. American Society of Nephrology 29th Annual Meeting. New Orleans, USA 11/1996
Autoantibodies to neutrophil granule proteins: pathogenic potential in vasculitis?
Locke, I.C. and Cambridge, G. 1996. Autoantibodies to neutrophil granule proteins: pathogenic potential in vasculitis? British Journal of Biomedical Science. 53 (4), pp. 302-316.
A human monoclonal anti-myeloperoxidase antibody can prime human neutrophils in vitro
Cambridge, G., Leaker, B., Locke, I.C., Noronha-Dutra, A. and Epperlein, M. 1996. A human monoclonal anti-myeloperoxidase antibody can prime human neutrophils in vitro. American Society of Nephrology 29th Annual Meeting. New Orleans, USA 11/1996
Autoantibodies to myeloperoxidase in systemic sclerosis
Worral, J.G., Locke, I.C., Leaker, B., Black, C.M. and Cambridge, G. 1995. Autoantibodies to myeloperoxidase in systemic sclerosis. British Society for Rheumatology Heberden Round 1995. Keele University, UK 9/1995
An automated method for the determination of deoxyribonuclease activity as exemplified by fractionation of the components of the medicament Varidase®
Locke, I.C., Ramsay, M.P., Hill, S.S. and Carpenter, B.G. 1993. An automated method for the determination of deoxyribonuclease activity as exemplified by fractionation of the components of the medicament Varidase®. The Journal of Automatic Chemistry. 15 242749.
Automated analysis of the deoxyribonuclease active constituents of the medicament Varidase ®
Locke, I.C., Ramsey, M.P., Hill, S.S. and Carpenter, B.G. 1992. Automated analysis of the deoxyribonuclease active constituents of the medicament Varidase ®. IMLS XX Triennial Conference. Liverpool, UK 9/1992
Purification of the seed lectin from Dolichos biflorus by chromatofocusing
Rogers, D.J., Locke, I.C., Gibbs, R. and Carpenter, B.G. 1990. Purification of the seed lectin from Dolichos biflorus by chromatofocusing. 12th International Lectin Conference. University of Davis, USA 9/1990
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