Simultaneous identification of common synthetic adulterants in slimming aids and sexual enhancers herbal supplements by High-performance Thin Layer Chromatography

Jalil, Banaz, Prieto, Jose M., Booker, Anthony and Heinrich, Michael 2021. Simultaneous identification of common synthetic adulterants in slimming aids and sexual enhancers herbal supplements by High-performance Thin Layer Chromatography. Planta Medica. 87 (15) 1243. https://doi.org/10.1055/s-0041-1736759

TitleSimultaneous identification of common synthetic adulterants in slimming aids and sexual enhancers herbal supplements by High-performance Thin Layer Chromatography
AuthorsJalil, Banaz, Prieto, Jose M., Booker, Anthony and Heinrich, Michael
Abstract

Introduction Herbal supplements are important and widely used health care choices but also pose a global challenge in quality and safety. The lack of regulations and less stringent value chains, for example, result in adulterations with undeclared synthetic substances. The extent and nature of this issue has been unknown in the Middle East.

Besides, analytical methods for easy implementation in laboratories without sophisticated instrumentations and limited resources are always in demand, particularly in developing countries. To the best of our knowledge, all proposed methodologies to date focused on identifying one class of synthetic adulterants, such as erectile dysfunction drugs [1] and anti-obesity drugs [2]. This project aimed to develop and validate a simple, rapid, robust, specific, reliable and cost-effective qualitative and quantitative HPTLC method.

Materials and Methods 40 samples were collected from pharmacies and herbal shops in Iraq, including those promoted for slimming and sexual enhancements. During method development, several steps were optimised, including the choice of stationary phase, mobile phase, samples extraction, injection volumes, detection and derivatisation.

Ethanol solvent system with silica gel as stationary phase was selected. It enabled a good separation of all reference standards (i.e., R F value ranged from 0.10 to 0.76, and injection volumes varied from 2 to 20 μl).

Results and Discussions The developed method was validated for specificity, precision, repeatability, and linearity. The limit of detection (148-1378μg), the limit of quantification (450-4176μg), and the regression coefficient was calculated. Out of the samples analysed, 48% (n=19) contained one adulterant; among these, 15% (n=6) contained two adulterants. [Figure 1] shows the percentage of adulterants identified in herbal supplements promoted for slimming and sexual enhancements (n=40).

Conclusions The use of supplements promoted for slimming and sexual enhancement could impose a health risk to the consumers due to common adulterations in such products. The method developed enables a efficient one-step detection of common adulterants.

Article number1243
JournalPlanta Medica
Journal citation87 (15)
ISSN1439-0221
Year2021
PublisherGeorg Thieme Verlag
Digital Object Identifier (DOI)https://doi.org/10.1055/s-0041-1736759
Publication dates
Published online13 Dec 2021

Related outputs

Chrysanthemum species used as food and medicine: Understanding quality differences on the global market
Gu, J., Scotti, F., Reich, E., Kirchhoff, R., Booker, A. and Heinrich, P.M. 2022. Chrysanthemum species used as food and medicine: Understanding quality differences on the global market. South African Journal of Botany. 148, pp. 123-134. https://doi.org/10.1016/j.sajb.2022.04.009

Green tea polyphenols in cardiometabolic health: A critical appraisal on Phytogenomics towards personalized green tea
Rajan, Logesh, Radhakrishnan, Arun, Alagarsamy, Keshav Narayan, Srivastava, Abhay, Dhingra, Sanjiv, Booker, Anthony, Rolfe, Viven, Palaniswamy, Dhanabal and Mohankumar, Suresh Kumar 2022. Green tea polyphenols in cardiometabolic health: A critical appraisal on Phytogenomics towards personalized green tea. PharmaNutrition. 20 100296. https://doi.org/10.1016/j.phanu.2022.100296

Emergent quality issues in Chinese medicinal plants: Investigation of their contemporary occurrence and historical persistence through questionnaires and interviews of key informants.
Fitzgerald, M., Heinrich, M. and Booker, A. 2021. Emergent quality issues in Chinese medicinal plants: Investigation of their contemporary occurrence and historical persistence through questionnaires and interviews of key informants. Planta Medica. 87 (15), pp. 1283-1284. https://doi.org/10.1055/s-0041-1736891

Quality differences of genus Chrysanthemum used as food and medicine from the global market
Gu, Jingyi, Reich, Eike, Kirchhof, Rainer, Scotti, Francesca, Booker, Anthony and Heinrich, Michael 2021. Quality differences of genus Chrysanthemum used as food and medicine from the global market. Planta Medica. 87 (15), pp. 1301-1302. https://doi.org/10.1055/s-0041-1736948

Treating Chronic Wounds Using Photoactive Metabolites: Data Mining the Chinese Pharmacopoeia for Potential Lead Species
Booker, A., Scotti, F., Mou, L., Huang, C., Werckerle, C., Maake, C. and Heinrich, M. 2021. Treating Chronic Wounds Using Photoactive Metabolites: Data Mining the Chinese Pharmacopoeia for Potential Lead Species. Planta Medica. 87, pp. 1-13. https://doi.org/10.1055/a-1578-8778

Medicinal plants from the Himalayan region as potential novel antimicrobial and anti-inflammatory skin treatments
Heinrich, M., Jiang, H., Scotti, F., Booker, A., Walt, H., Weckerle, C. and Maake, C. 2021. Medicinal plants from the Himalayan region as potential novel antimicrobial and anti-inflammatory skin treatments. Journal of Pharmacy and Pharmacology. 73 (7), p. 956–967. https://doi.org/10.1093/jpp/rgab039

Mitochondrial Function as a Potential Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas
Woodley, S., Mould, R., Sahuri Arisoylu, M., Kalampouka, I., Booker, A. and Bell, J.D. 2021. Mitochondrial Function as a Potential Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas. Frontiers in Pharmacology. 12 660938. https://doi.org/10.3389/fphar.2021.660938

Chemical Authentication of Botanical Ingredients: A Review of Commercial Herbal Products
Ichim, M.C. and Booker, A. 2021. Chemical Authentication of Botanical Ingredients: A Review of Commercial Herbal Products. Frontiers in Pharmacology. 12 666850. https://doi.org/10.3389/fphar.2021.666850

Medicinal plant analysis: A historical and regional discussion of Chinese publication trends and emergent complex techniques
Fitzgerald, M., Heinrich, M. and Booker, A. 2020. Medicinal plant analysis: A historical and regional discussion of Chinese publication trends and emergent complex techniques. Frontiers in Pharmacology. 10 1480. https://doi.org/10.3389/fphar.2019.01480

Comprehensive HPTLC fingerprinting as a tool for a simplified analysis of purity of ginkgo products
Frommenwiler, D., Booker, A., Vila, R., Heinrich, M., Reich, E. and Cañiguerala, S. 2019. Comprehensive HPTLC fingerprinting as a tool for a simplified analysis of purity of ginkgo products. Journal of Ethnopharmacology. 243, p. 112084 112084. https://doi.org/10.1016/j.jep.2019.112084

Turmeric (Curcuma longa L.) products: What quality differences exist?
Chatzinasiou, L., Booker, A., MacLennan, E., Mackonochie, M. and Heinrich, M. 2019. Turmeric (Curcuma longa L.) products: What quality differences exist? Journal of Herbal Medicine. 17-18, p. 100281 100281. https://doi.org/10.1016/j.hermed.2019.100281

Unblocking high-value botanical value chains: Is there a role for blockchain systems?
Booker, A., Heinrich, M., Scotti, F., Fitzgerald, M., Kum, K.Y. and Lobel, K. 2019. Unblocking high-value botanical value chains: Is there a role for blockchain systems? Frontiers in Pharmacology. 10 396. https://doi.org/10.3389/fphar.2019.00396

Quality Control of Hypericum perforatum L. Analytical challenges and recent progress
Agapouda, A., Booker, A., Kiss, T., Hohmann, J., Heinrich, M. and Csupor, D. 2019. Quality Control of Hypericum perforatum L. Analytical challenges and recent progress. Journal of Pharmacy and Pharmacology. 71 (1), pp. 15-37. https://doi.org/10.1111/jphp.12711

Complexity in Chinese herbal medicine supply chains
Booker, A., Fitzgerald, M., Heinrich, P.M. and Scheid, V. 2018. Complexity in Chinese herbal medicine supply chains. 66th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) jointly with the 11th Shanghai TCM conference. Shanghai, PRC 26 - 29 Aug 2018

Domestication Study of Senecio nutans Sch.Bip. (Asteraceae): its Economic Potential and Sustainable Management
Lira, A., Crossley, L., Parra, C., Echiburú, C., Booker, A. and Heinrich, M. 2018. Domestication Study of Senecio nutans Sch.Bip. (Asteraceae): its Economic Potential and Sustainable Management. 66th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) jointly with the 11th Shanghai TCM conference. Shanghai, PRC 26 - 29 Aug 2018

St. John’s Wort (Hypericum perforatum) products – How variable is the primary material?
Scotti, F., Lobel, K., Booker, A. and Heinrich, M. 2018. St. John’s Wort (Hypericum perforatum) products – How variable is the primary material? Frontiers in Plant Science. 9 1973. https://doi.org/10.3389/fpls.2018.01973

St John’s wort (Hypericum perforatum) products – an assessment of their authenticity and quality
Booker, A., Agapouda, A., Frommenwiler, D.A., Scotti, F., Reich, E. and Heinrich, P.M. 2018. St John’s wort (Hypericum perforatum) products – an assessment of their authenticity and quality. Phytomedicine. 40, pp. 158-164 PHYMED52314. https://doi.org/10.1016/j.phymed.2017.12.012

Book Review: Chinese Medicinal Plants, Herbal Drugs and Substitutes, an identification guide
Booker, A. 2018. Book Review: Chinese Medicinal Plants, Herbal Drugs and Substitutes, an identification guide. Journal of Ethnopharmacology. 213, p. 198. https://doi.org/10.1016/j.jep.2017.11.017

Useful Plants - Medicines
Booker, A., Allkin, B., Patmore, K., Black, N., Canteiro, C., Dauncy, E., Edwards, S., Forest, F., Giovannini, P., Howes, M.J., Hudson, A., Irving, J., Leon, C., Milliken, W., Lughadha, E.N., Schippmann, U. and Simmonds, M.S.J. 2017. Useful Plants - Medicines. in: Willis, K.J. (ed.) The State of the World's Plants. Report London, UK The Board of Trustees of the Royal Botanic Gardens, Kew. pp. 22-29

Collectors of Rhodiola species on the Sichuan-Tibetan and Sichuan-Shaanxi borders
Booker, A., Springer, L. and Heinrich, M. 2016. Collectors of Rhodiola species on the Sichuan-Tibetan and Sichuan-Shaanxi borders. The 16th International Congress on Ethnopharmacology. Yulin, China 15 - 18 May 2016 International Society for Ethnopharmacology.

Comparison of different Rhodiola species using NMR- metabolomics, HPTLC and DNA barcoding techniques
Booker, A., Zhai, L., Gkouva, C., Li, S., Frommenwiler, D., Reich, E., Howard, C., Sgamma, T., Williams, S., Slater, A. and Heinrich, M. 2016. Comparison of different Rhodiola species using NMR- metabolomics, HPTLC and DNA barcoding techniques. 9th Joint Natural Products Conference. Copenhagen, Denmark 24 - 27 Jul 2016 Georg Thieme Verlag. https://doi.org/10.1055/s-0036-1597026

Value Chains of Botanical and Herbal Medicinal Products: A European Perspective
Booker, A. and Heinrich, M. 2016. Value Chains of Botanical and Herbal Medicinal Products: A European Perspective. HerbalGram. 112, pp. 40-45.

From traditional resource to global commodities: a comparison of Rhodiola species using NMR spectroscopy - metabolomics and HPTLC
Booker, A., Zhai, L., Gkouva, C., Li, S. and Heinrich, M. 2016. From traditional resource to global commodities: a comparison of Rhodiola species using NMR spectroscopy - metabolomics and HPTLC. Frontiers in Pharmacology. 7 254. https://doi.org/10.3389/fphar.2016.00254

Adulteration and Poor Quality of Ginkgo biloba Supplements
Booker, A., Frommenwiler, D., Reich, E., Horsfield, S. and Heinrich, M. 2016. Adulteration and Poor Quality of Ginkgo biloba Supplements. Journal of Herbal Medicine. 6 (2), pp. 79-87. https://doi.org/10.1016/j.hermed.2016.04.003

Chinese Medicine: Contentions and Global Complexities
Booker, A. 2015. Chinese Medicine: Contentions and Global Complexities. in: Heinrich, M. and Jäger, A.K. (ed.) Ethnopharmacology New York, United States Wiley. pp. 293-302

Permalink - https://westminsterresearch.westminster.ac.uk/item/vq4y9/simultaneous-identification-of-common-synthetic-adulterants-in-slimming-aids-and-sexual-enhancers-herbal-supplements-by-high-performance-thin-layer-chromatography


Share this

Usage statistics

36 total views
0 total downloads
7 views this month
0 downloads this month
These values are for the period from September 2nd 2018, when this repository was created