Title | Improving Physiological Relevance of Cell Culture: The Possibilities, Considerations and Future Directions of the Ex Vivo Co-Culture Model |
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Type | Journal article |
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Authors | Allen, Sophie L., Elliott, Bradley T., Carson, Brian P. and Breen, Leigh |
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Abstract | In vitro models provide an important platform for the investigation of cellular growth and atrophy to inform, or extend mechanistic insights from, logistically challenging in vivo trials. While these models allow for the identification of candidate mechanistic pathways, many models involve supraphysiological dosages, non-physiological conditions, or experimental changes relating to individual proteins or receptors, all of which limit translation to human trials. To overcome these drawbacks, the use of ex vivo human plasma and serum has been used in cellular models to investigate changes in myotube hypertrophy, cellular protein synthesis, anabolic and catabolic markers in response to differing age, disease states, and nutrient status. However, there are currently no concurrent guidelines outlining the optimal methodology for this model. This review discusses the key methodological considerations surrounding the use of ex vivoplasma and serum, with a focus in application to skeletal muscle cell lines (i.e., C2C12, L6 and LHCN-M2) and human primary skeletal muscle cells (HSMC) as a means to investigate molecular signaling in models of atrophy and hypertrophy, alongside future directions. |
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Keywords | Cell Biology |
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| Physiology |
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Journal | American Journal of Physiology: Cell Physiology |
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Journal citation | 324 (2), pp. C420-C427 |
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ISSN | 0363-6143 |
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| 1522-1563 |
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Year | 2023 |
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Publisher | American Physiological Society |
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Accepted author manuscript | File Access Level Open (open metadata and files) |
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Publisher's version | License CC BY 4.0 File Access Level Open (open metadata and files) |
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Digital Object Identifier (DOI) | https://doi.org/10.1152/ajpcell.00473.2022 |
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PubMed ID | 36571441 |
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Publication dates |
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Published online | 26 Dec 2022 |
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Published in print | Feb 2023 |
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Project | BRC-1215-2009 |
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Funder | NIHR | Birmingham Biomedical Research Centre |
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| Department of Agriculture Food and the Marine |
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| The George Cayley Circle |
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