An automated panel for assessing pro-oxidant and antioxidant status in human serum

Ferrier, C., Reed, S.C. and Elliott, B. 2017. An automated panel for assessing pro-oxidant and antioxidant status in human serum.

TitleAn automated panel for assessing pro-oxidant and antioxidant status in human serum
AuthorsFerrier, C., Reed, S.C. and Elliott, B.
TypeProject report
Abstract

Background: The implied role of reactive oxygen species in diabetes, cardiovascular disease and some forms of cancer suggests that the measurement of pro-oxidant and antioxidant biomarkers may be a useful addition to current routine tests.
Methods: Validation procedures were undertake to establish performance characteristics for glutathione peroxidase, glutathione reductase, superoxide dismutase and total antioxidant status assays on the ILab Aries analyser. Blood samples from thirty healthy participants enabled the determination of preliminary reference ranges for these tests in addition to the calculated antioxidant gap.
Results: The performance of all the tests was favourably comparable with that stated by the manufacturer. Preliminary reference ranges were similar to those in previous publications. There was a requirement for separate male and female ranges for all the panel tests apart from gamma-glutamyltransferase.
Conclusions: An automated panel for selected serum pro-oxidant and antioxidant biomarkers is feasible in terms of test performance, turnaround time and costs. Additional studies on larger groups of individuals with known disorders will determine the clinical usefulness of this panel.

KeywordsOxidative Stress
Antioxidants
Glutathione Reductase
Glutathione Peroxidase
gamma-Glutamyltransferase
Uric Acid
Year2017
Publication dates
Published2017

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