Armson, B.L., Wright, E., McElhinney, L.M., Banyard, A.C., Goddard, T., Healy, D.M., Voller, K., Weiss, R.A. and Fooks, A.R. 2010. Development of a novel serological assay for the detection of rabies virus neutralising antibodies using lentiviral pseudotypes. 4th Annual Meeting EPIZONE. Saint Malo, France 2010
| Title | Development of a novel serological assay for the detection of rabies virus neutralising antibodies using lentiviral pseudotypes |
|---|---|
| Authors | Armson, B.L., Wright, E., McElhinney, L.M., Banyard, A.C., Goddard, T., Healy, D.M., Voller, K., Weiss, R.A. and Fooks, A.R. |
| Type | Conference paper |
| Abstract | Rabies virus (RABV) causes an acute encephalitis that is almost invariably fatal. Whilst effective vaccines and post exposure prophylaxis are available it is estimated that over 55,000 human fatalities occur worldwide each year. This figure is, however, believed to be a gross underestimation of the actual number of human deaths, as a disproportionate number of fatalities occur across the developing world where reporting systems are mainly absent. The detection of virus neutralising antibodies (VNA) is key to the evaluation of vaccination status following pre-immunisation. Current tests for RABV VNAs require expensive reagents and equipment, along with biosafety containment level 3 facilities, something not easily achieved in rabies endemic areas. The use of lentiviral pseudotypes as a vector for gene therapy is well documented. These have proven to be highly sensitive yet flexible platforms which can be adapted to allow the evaluation of vaccines and antiviral drugs against highly pathogenic viruses without the need for high level containment facilities or great expense. We have adapted this technology to allow the determination of serum neutralising antibody titres against highly pathogenic rabies and related lyssaviruses. The pseudotype assay only requires a small amount of serum in comparison to the standard fluorescent virus neutralisation assay (FAVN), and the use of different reporter genes, such as green fluorescent protein, luciferase, or β-galactosidase, makes it possible for the assay to be undertaken at low cost in laboratories throughout the world. G-protein sequences from viral isolates representing each lyssavirus genotype and the newly classified Eurasian strains were cloned and co-expressed with lentiviral gag-pol and different reporter genes. The pseudotypes infected a number of target cell lines and produced titres almost equivalent to VSV-G protein pseudotypes. To date, over 500 sera have been evaluated concurrently using genotype 1, CVS-11 live virus (FAVN) and pseudotype neutralisation assays. Comparison of antibody titres reveals a 96.4% sensitivity and 100% specificity (r=0.83, p<0.001). Neutralisation assays using pseudotypes with glycoproteins from other lyssavirus genotypes suggest that they provide a greater sensitivity compared to the current virus neutralization tests and will therefore allow a more accurate determination of serological response to these highly pathogenic infections. Importantly for the use of this assay in countries where the cold chain cannot be maintained, CVS-11 pseudotypes were highly stable during freeze-thaw cycles and storage at room temperature. These results suggest that the proposed pseudotype assay is a practical, effective and robust solution for rapid lyssavirus serosurveillance wherever it is needed. |
| Year | 2010 |
| Conference | 4th Annual Meeting EPIZONE |
| Publication dates | |
| Published | 2010 |
Human papilloma virus genotype distribution and risk factor analysis amongst reproductive aged women in urban Gambia
Bah Camara, H., Anyanwu, M., Wright, E. and Kimmitt, P.T. 2018. Human papilloma virus genotype distribution and risk factor analysis amongst reproductive aged women in urban Gambia. Journal of Medical Microbiology. 67, pp. 1645-1654. https://doi.org/10.1099/jmm.0.000848
Efficacy Of The Quadrivalent HPV Vaccine In Cervical Cancer Prevention Strategy In The Gambia
Bah Camara, H., Anyanwu, M., Banja, F., Mboob-Suun, K., Ceesay, F., Wright, E. and Kimmitt, P.T. 2017. Efficacy Of The Quadrivalent HPV Vaccine In Cervical Cancer Prevention Strategy In The Gambia. EUROGIN17. Amsterdam 08 - 11 Oct 2017
A Preliminary Report: Cervical Human Papilloma Virus Genotype Distribution in Reproductive Aged Women Attending Primary Health Care in Urban Gambia
Bah Camara, H., Mboob -Suun, K., Banja, F., Ceesay, F., Anyanwu, M., Wright, E. and Kimmitt, P.T. 2017. A Preliminary Report: Cervical Human Papilloma Virus Genotype Distribution in Reproductive Aged Women Attending Primary Health Care in Urban Gambia. Microbiology Society Annual Conference 2017. Edinburgh 03 - 06 Apr 2017
Prevalance of Ureaplasma parvum and urealyticum in Urban Gambian Women
Bah Camara, H., Noho, A., Sanyang, A., Barrow, E., Wright, E. and Kimmitt, P.T. 2017. Prevalance of Ureaplasma parvum and urealyticum in Urban Gambian Women. Microbiology Society Annual Conference 2017. Edinburgh 03 - 06 Apr 2017
Planning for the unexpected: Ebola virus, Zika virus, what's next?
Welfare, W. and Wright, E. 2016. Planning for the unexpected: Ebola virus, Zika virus, what's next? British Journal of Hospital Medicine. 77 (12), pp. 704-707. https://doi.org/10.12968/hmed.2016.77.12.704
Ebolavirus pseudotypes as antigen surrogates for serological studies
Bentley, E., Mattiuzzo, G., Wash, R., Binter, S., Friedrich, M., Goulding, D., Kellam, P., Page, M. and Wright, E. 2016. Ebolavirus pseudotypes as antigen surrogates for serological studies. International Journal of Infectious Diseases. 53 (Supplement), p. 123. https://doi.org/10.1016/j.ijid.2016.11.306
Exploiting viral pseudotypes for emerging virus research
Grehan, K., Bentley, E., Mather, S., Kinsley, R., Scott, S.D., Wright, E. and Temperton, N.J. 2016. Exploiting viral pseudotypes for emerging virus research. International Journal of Infectious Diseases. 53 (Supplement), p. 8. https://doi.org/10.1016/j.ijid.2016.11.024
Minimal In Vivo Efficacy of Iminosugars in a Lethal Ebola Virus Guinea Pig Model
Miller, J.L., Spiro, S.G., Dowall, S.D., Taylor, I., Rule, A., Alonzi, D.S., Sayce, A.C., Wright, E., Bentley, E.M., Thom, R., Hall, G., Dwek, R.A., Hewson, R. and Zitzmann, N. 2016. Minimal In Vivo Efficacy of Iminosugars in a Lethal Ebola Virus Guinea Pig Model. PLoS ONE. 11 (11) e0167018. https://doi.org/10.1371/journal.pone.0167018
A monovalent chimpanzee adenovirus Ebola vaccine boosted with MVA
Ewer, K., Rampling, T., Venkatraman, N., Bowyer, G., Wright, D, Lambe, T., Imoukhuede, E.B., Payne, R., Fehling, S.K., Strecker, T., Biedenkopf, N., Krähling, V., Tully, C.M., Edwards, N.J., Bentley, E.M., Samuel, D., Labbé, G., Jin, J., Gibani, M., Minhinnick, A., Wilkie, M., Poulton, I., Lella, N., Roberts, R., Hartnell, F., Bliss, C., Sierra-Davidson, K., Powlson, J., Berrie, E., Tedder, R., Roman, F., De Ryck, I., Nicosia, A., Sullivan, N.J., Stanley, D.A., Mbaya, O.T., Ledgerwood, J.E., Schwartz, R.M., Siani, L., Colloca, S., Folgori, A., Di Marco, S., Cortese, R., Wright, E., Becker, S., Graham, B.S., Koup, R.A., Levine, M.M., Volkmann, A., Chaplin, P., Pollard, A.J., Draper, S.J., Ballou, W.R., Lawrie, A., Gilbert, S.C. and Hill, A.V.S. 2016. A monovalent chimpanzee adenovirus Ebola vaccine boosted with MVA. The New England Journal of Medicine. 374 (17), pp. 1635-1646. https://doi.org/10.1056/NEJMoa1411627
Development of broad-spectrum human monoclonal antibodies for rabies post-exposure prophylaxis
Wright, E., Benedictis Paola, D., Minola, A., Rota Nodari, E., Aiello, R., Zecchin, B., Salomoni, A., Foglierini, M., Agatic, G., Vanzetta, F., Lavenir, R., Lepelletier, A., Bentley, E., Weiss, R.A., Cattoli, G., Capua, I., Sallusto, F., Lanzavecchia, A., Bourhy, H., Corti, D. and De Benedictis, P. 2016. Development of broad-spectrum human monoclonal antibodies for rabies post-exposure prophylaxis. EMBO Molecular Medicine. 8 (4), pp. 407-421. https://doi.org/10.15252/emmm.201505986
Technical considerations for the generation of novel pseudotyped viruses
King, B., Temperton, N.J., Grehan, K., Scott, S.D., Wright, E., Tarr, A.W. and Daly, J.M. 2016. Technical considerations for the generation of novel pseudotyped viruses. Future Virology. 11 (1), pp. 47-59. https://doi.org/10.2217/fvl.15.106
Retroviral Pseudotypes – From Scientific Tools to Clinical Utility
Wright, E., Temperton, N.J. and Scott, S.D. 2015. Retroviral Pseudotypes – From Scientific Tools to Clinical Utility. in: Encyclopedia of life sciences Chichester Wiley. pp. 1-11
Antiviral therapies against Ebola and other emerging viral diseases using existing medicines that block virus entry
Long, J., Wright, E., Molesti, E., Temperton, N. and Barclay, W. 2015. Antiviral therapies against Ebola and other emerging viral diseases using existing medicines that block virus entry. F1000Research. 4 (30). https://doi.org/10.12688/f1000research.6085.2
Bat and pig Interferon-Induced Transmembrane Protein 3 restrict cell entry by influenza virus and lyssaviruses
Benfield, C., Smith, S.E., Wright, E., Wash, R.S., Ferrara, F., Temperton, N.J. and Kellam, P. 2015. Bat and pig Interferon-Induced Transmembrane Protein 3 restrict cell entry by influenza virus and lyssaviruses. Journal of General Virology. 96 (5), pp. 991-1005. https://doi.org/10.1099/vir.0.000058
Demonstration of viral antibodies by pseudotype virus neutralisation
Wright, E. 2014. Demonstration of viral antibodies by pseudotype virus neutralisation. in: Rupprecht, C. and Nagarajan, T. (ed.) Current laboratory techniques in rabies diagnosis, research and prevention Elsevier. pp. 147-156
Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies
Molesti, E., Wright, E., Terregino, C., Rahman, R., Cattoli, G. and Temperton, N.J. 2014. Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies. Journal of Immunology Research. 2014 457932. https://doi.org/10.1155/2014/457932
Lyophilisation of influenza, rabies and Marburg lentiviral pseudotype viruses for the development and distribution of a neutralisation-assay based diagnostic kit
Mather, S., Wright, E., Scott, S. and Temperton, N.J. 2014. Lyophilisation of influenza, rabies and Marburg lentiviral pseudotype viruses for the development and distribution of a neutralisation-assay based diagnostic kit. Journal of Virological Methods. 210, pp. 51-58. https://doi.org/10.1016/j.jviromet.2014.09.021
Current progress with serological assays for exotic emerging/re-emerging viruses
Mather, S., Scott, S., Temperton, N.J., Wright, E., King, B. and Daly, J.M. 2013. Current progress with serological assays for exotic emerging/re-emerging viruses. Future Virology. 8 (8), pp. 745-755. https://doi.org/10.2217/fvl.13.60
Production, characterization, and antigen specificity of recombinant 62-71-3, a candidate monoclonal antibody for rabies prophylaxis in humans
Both, L., van Dolleweerd, C., Wright, E., Banyard, A.C., Bulmer-Thomas, B., Selden, D., Altmann, F., Fooks, A.R. and Ma, J.K.C. 2013. Production, characterization, and antigen specificity of recombinant 62-71-3, a candidate monoclonal antibody for rabies prophylaxis in humans. FASEB Journal. 27 (5), pp. 2055-2065. https://doi.org/10.1096/fj.12-219964
Development of a pseudotype-based neutralisation assay kit for in-field vaccine evaluation and sero-surveillance of highly pathogenic viruses
Temperton, N.J., Mather, S., Scott, S. and Wright, E. 2013. Development of a pseudotype-based neutralisation assay kit for in-field vaccine evaluation and sero-surveillance of highly pathogenic viruses. Influenza 2013: One Influenza, One World, One health. Oxford, UK September 2013
Development of a pseudotype neutralisation assay-based diagnostic kit for in-field vaccine evaluation and serosurveillance for highly pathogenic viruses
Mather, S., Temperton, N.J., Wright, E. and Scott, S. 2013. Development of a pseudotype neutralisation assay-based diagnostic kit for in-field vaccine evaluation and serosurveillance for highly pathogenic viruses. Society For General Microbiology Spring Meeting. Manchester, UK March 2013
Investigating the antibody neutralisation potential of a rabies virus lentiviral pseudotype: an international inter-laboratory trial
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The lyssavirus glycoprotein: how significant are the currently characterised antigenic sites in the induction of neutralising antibodies?
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Investigating the functionality and antigenicity of chimeric lyssavirus glycoproteins and their neutralisation profiles
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Investigating the functionality and antigenicity of chimeric lyssavirus glycoproteins and their neutralisation profiles
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Characterization of a monoclonal antibody cocktail for human rabies prophylaxis
Both, L., van Dolleweerd, C., Banyard, A.C., Wright, E., Fooks, A.R. and Ma, J.K.C. 2013. Characterization of a monoclonal antibody cocktail for human rabies prophylaxis. Pseudotype viruses: applications and troubleshooting. London, UK October 2013
Monoclonal antibodies for prophylactic and therapeutic use against viral infections
Both, L., Banyard, A.C., van Dolleweerd, C., Wright, E., Ma, J.K.C. and Fooks, A.R. 2013. Monoclonal antibodies for prophylactic and therapeutic use against viral infections. Vaccine. 31 (12), pp. 1553-1559. https://doi.org/10.1016/j.vaccine.2013.01.025
Single domain antibody multimers confer protection against rabies infection
Boruah, B.M., Liu, D., Ye, D., Gu, T.J., Jiang, C.L., Qu, M., Wright, E., Wang, W., He, W., Liu, C. and Gao, B. 2013. Single domain antibody multimers confer protection against rabies infection. PLoS ONE. 8 (8) e71383. https://doi.org/10.1371/journal.pone.0071383
The construction of chimeric rabies virus glycoproteins rescues Arctic-like rabies pseudovirus production
Bentley, E., Ali, R., Horton, D.L., Banyard, A.C. and Wright, E. 2013. The construction of chimeric rabies virus glycoproteins rescues Arctic-like rabies pseudovirus production. Pseudotype viruses: applications and troubleshooting. London, UK October 2013
The construction of chimeric rabies virus glycoproteins rescues Arctic-like rabies pseudovirus production
Ali, R., Horton, D.L., Banyard, A.C. and Wright, E. 2013. The construction of chimeric rabies virus glycoproteins rescues Arctic-like rabies pseudovirus production. Society For General Microbiology Spring Meeting. Manchester, UK March 2013
Chicken IFITM3 restricts influenza viruses and lyssaviruses in vitro
Smith, S.E., Gibson, M.S., Wash, R.S., Ferrara, F., Wright, E., Temperton, N.J., Kellam, P. and Fife, M. 2013. Chicken IFITM3 restricts influenza viruses and lyssaviruses in vitro. Journal of Virology. 87 (23), pp. 12957 -12966. https://doi.org/10.1128/JVI.01443-13
Henipavirus neutralising antibodies in an isolated island population of African fruit bats
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Investigating antigenic epitopes on the lyssavirus glycoprotein
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Coreceptor and cytokine concentrations may not explain differences in disease progression observed in HIV-1 clade A and D infected Ugandans
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CL-184, a combination of two human monoclonal antibodies, for use as a vaccine for the potential postexposure prophylaxis of rabies
Wright, E. 2011. CL-184, a combination of two human monoclonal antibodies, for use as a vaccine for the potential postexposure prophylaxis of rabies. Thomson Reuters.
Characterization of a plant-derived monoclonal antibody cocktail for human rabies prophylaxis
Both, L., van Dolleweerd, C., Banyard, A.C., Wright, E., Fooks, A.R. and Ma, J.K.C. 2011. Characterization of a plant-derived monoclonal antibody cocktail for human rabies prophylaxis. Rabies In The Americas XXII. San Juan, Puerto Rico 2011
Characterization of a monoclonal antibody cocktail for human rabies prophylaxis
Both, L., van Dolleweerd, C., Banyard, A.C., Wright, E., Fooks, A.R. and Ma, J.K.C. 2011. Characterization of a monoclonal antibody cocktail for human rabies prophylaxis. 1. 4th International Conference in Plant-Based Vaccines and Antibodies. Porto, Portugal 2011
The efficacy of current vaccines and novel Nanobody-based antivirals against highly pathogenic rabies and lyssaviruses
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The efficacy of current rabies vaccines and novel Nanobody®-based antivirals against highly pathogenic phylogroup -1 and -2 members of the Lyssavirus genus
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Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses
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A robust lentiviral pseudotype neutralisation assay for in-field serosurveillance of rabies and lyssaviruses in Africa
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Emerging technologies for the detection of rabies virus: challenges and hopes in the 21st century
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A robust lentiviral pseudotype neutralisation assay for rabies and lyssavirus serology
Wright, E., Temperton, N.J., Weiss, R.A., McNabb, S., Lembo, T., Cleaveland, S., Marston, D.A., Horton, D.L., McElhinney, L.M. and Fooks, A.R. 2009. A robust lentiviral pseudotype neutralisation assay for rabies and lyssavirus serology. Rabies In The Americas XX. Quebec, Canada 2009
The implications for ‘in-field’ sero-surveillance and evaluation of vaccines targeting rabies using lacZ based lentiviral pseudotypes
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Retroviral pseudotypes
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HIV blocking antibodies following immunisation with chimaeric peptides coding a short N-terminal sequence of the CCR5 receptor
Chain, B.M., Noursadeghi, M., Gardener, M., Tsang, J. and Wright, E. 2008. HIV blocking antibodies following immunisation with chimaeric peptides coding a short N-terminal sequence of the CCR5 receptor. Vaccine. 26 (45), pp. 5752-5759. https://doi.org/10.1016/j.vaccine.2008.08.025
Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison
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Duffy Antigen Receptor for Chemokines mediates trans-infection of HIV-1 from red blood cells to target cells and affects HIV-AIDS susceptibility
He, W., Neil, S., Kulkarni, H., Wright, E., Agan, B.K., Marconi, V.C., Dolan, M.J., Weiss, R.A. and Ahuja, S.K. 2008. Duffy Antigen Receptor for Chemokines mediates trans-infection of HIV-1 from red blood cells to target cells and affects HIV-AIDS susceptibility. Cell Host and Microbe. 4 (1), pp. 52-62. https://doi.org/10.1016/j.chom.2008.06.002
A novel serological assay for the detection of rabies virus and lyssavirus neutralising antibodies
Wright, E., Temperton, N.J., Marston, D.A., Fooks, A.R. and Weiss, R.A. 2008. A novel serological assay for the detection of rabies virus and lyssavirus neutralising antibodies. Society For General Microbiology Spring 2008 Meeting. Edinburgh, UK 2008
The evaluation of antibody responses to rabies virus using retroviral pseudotypes and the implications for ‘in-field’ vaccine trials
Wright, E., Temperton, N.J., Marston, D.A., Fooks, A.R. and Weiss, R.A. 2008. The evaluation of antibody responses to rabies virus using retroviral pseudotypes and the implications for ‘in-field’ vaccine trials. BIT's First Annual World Vaccine Congress. Foshan, China 2008
Association between active GB virus-C (hepatitis G) infection and HIV-1 disease in Uganda
Yirrell, D.L., Wright, E., Shafer, L.A., Campbell, E., Van der Paal, L., Kaleebu, P., Grosskurth, H. and Whitworth, J.A. 2007. Association between active GB virus-C (hepatitis G) infection and HIV-1 disease in Uganda. International Journal of STD & AIDS. 18 (4), pp. 244-249. https://doi.org/10.1258/095646207780659006
A novel serological assay for detection of rabies virus neutralising antibodies
Wright, E., Temperton, N.J., Weiss, R.A., Marston, D.A., McElhinney, L.M. and Fooks, A.R. 2007. A novel serological assay for detection of rabies virus neutralising antibodies. Rabies In The Americas XVIII. Ciudad de Guanajuato, Mexico 2007
Characterisation of persistent viral infections in bats
Wright, E., Takeuchi, Y., Brookes, S., Fooks, A.R. and Weiss, R.A. 2007. Characterisation of persistent viral infections in bats. The First International Meeting on Emerging Diseases and Surveillance. Vienna, Austria 2007
Effect of Duffy antigen receptor for chemokine (DARC) polymorphisms on susceptibility to HIV infection and progression to AIDS
Wright, E., He, W., Neil, S., Kulkarni, H., Agan, B.K., Marconi, V.C., Dolan, M.J., Ahuja, S.K. and Weiss, R.A. 2007. Effect of Duffy antigen receptor for chemokine (DARC) polymorphisms on susceptibility to HIV infection and progression to AIDS. Institute Pasteur Genetics and Mechanisms of Susceptibility to Infectious Diseases. Paris, France 2007
Ets-2 repressor factor recruits histone deacetylase to silence human cytomegalovirus immediate-early gene expression in non-permissive cells
Wright, E., Bain, M., Teague, L., Murphy, J. and Sinclair, J. 2005. Ets-2 repressor factor recruits histone deacetylase to silence human cytomegalovirus immediate-early gene expression in non-permissive cells. Journal of General Virology. 86 (3), pp. 535-544. https://doi.org/10.1099/vir.0.80352-0
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