|Title||The implications for ‘in-field’ sero-surveillance and evaluation of vaccines targeting rabies using lacZ based lentiviral pseudotypes|
|Authors||Wright, E., McNabb, S., Goddard, T., Horton, D.L., Weiss, R.A., Cleaveland, S. and Fooks, A.R.|
We analysed 304 serum samples drawn from Tanzanian dogs in the widely used fluorescent antibody virus neutralisation assay (FAVN) and a neutralisation assay using pseudotypes bearing rabies G-proteins. An overall specificity of 100%, sensitivity of 94.4% and strong correlation (r2=0.915) were observed. Using lacZ as the pseudotype reporter did not effect the correlation (r2=0.918) and allowed detection without the need for expensive reagents or equipment. Freeze-thaw cycles decreased rabies pseudotype viral titre by 3.7%/cycle and storage at room temperature for a week resulted in a 22.6% decrease. This robust assay can also be applied to other highly pathogenic infections.
|Conference||Society For General Microbiology Spring 2009 Meeting|