|Title||Isatin (indole-2,3-dione) in urine and tissues: detection and determination by gas chromatography—mass spectrometry|
|Authors||Halket, J., Watkins, P.J., Przyborowska, A., Goodwin, B.L., Clow, A., Glover, V. and Sandler, M.|
A simple procedure based upon capillary column gas chromatography-mass spectrometry (GC—MS) is described for the detection and determination of isatin (indole-2,3-dione) in body fluids and tissues. After addition of 5-methylisatin as internal standard to urine or tissue homogenates, organic extracts are dried and derivatized successively with hydroxylamine hydrochloride and the reagent N-tert.-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA). The tert.-butyldimethylsilyl derivatives obtained show good GC—MS properties and allow quantification by selected-ion monitoring of m/z 333 (isatin) and m/z 347 (internal standard). Adult and newborn human urine output values lie in the ranges 0.4–3.2 mg/mmol of creatinine (5–30 mg per 24 h) and 0.002–0.518 mg/mmol of creatinine, respectively. There is a discontinuous regional distribution in rat tissues. The GC—MS properties of a number of derivatives formed by successive reaction of isatin with hydroxylamine hydrochloride (or methoxyaminehydrochloride or ethoxyamine hydrochloride) and MTBSTFA, bis(trimethylsilyl)trifluoroacetamide, pentafluoropropionic anhydride or pentafluorobenzyl bromide are also described.
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Journal citation||562 (1-2), pp. 279-287|
|Year||02 Jan 1991|
|Digital Object Identifier (DOI)||https://doi.org/10.1016/0378-4347(91)80585-Z|
|Published||02 Jan 1991|