Title | Regional and molecular separation of the four bioactivities of ‘tribulin’ |
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Authors | Hucklebridge, F., Doyle, A., Pang, F.Y., Adlard, M., Evans, P. and Clow, A. |
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Abstract | The endocoid tribulin has four known bioactivities: monoamine oxidase A and B inhibitory activities (MAO-AI and MAO-BI) and peripheral and central benzodiazepine receptor binding inhibitory activities (PBR-I and CBR-I). Analysis of the four bioactivities in rat tissue reveals regional dissociation. Most notably liver was particularly rich in PBR-I yet contained no detectable MAO-AI. In addition we have succeeded in separating the four activities from human urine. MAO-AI and MAO-BI have greater retention on charcoal than PBR-I and CBR-I when eluted step-wise with aqueous methanol. MAO-AI can be separated from MAO-BI and in addition PBR-I can be separated from CBR-I by step aqueous methanol elution from Amberlite XAD-4. Hence we present two lines of evidence that, contrary to the original view, tribulin is composed of a number of distinct molecular components. |
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Journal | Neuroscience Letters |
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Journal citation | 240 (1), pp. 29-32 |
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ISSN | 0304-3940 |
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Year | 02 Jan 1998 |
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Publisher | Elsevier |
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Digital Object Identifier (DOI) | https://doi.org/10.1016/S0304-3940(97)00916-6 |
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Publication dates |
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Published | 02 Jan 1998 |
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