Fluorescence in situ Hybridization (FISH): challenges and opportunities in the post-genomic era

Volpi, E. 2017. Fluorescence in situ Hybridization (FISH): challenges and opportunities in the post-genomic era. Applications of in situ hybridisation in research and disease. Cancer Research UK Cambridge Institute, Cambridge 17 - 18 May 2017

TitleFluorescence in situ Hybridization (FISH): challenges and opportunities in the post-genomic era
AuthorsVolpi, E.
TypeConference paper
Abstract

Fluorescence in situ hybridization (FISH) is a highly versatile laboratory technique combining the power of molecular analysis with details gained by fluorescence microscopy inspection at the single cell level. Initially introduced as a physical mapping technique for DNA sequence assembly at the time of the human genome project, FISH has re-invented itself repeatedly through time and, by doing so, succeeded in remaining at the forefront of research and diagnostic practice. In its various permutations FISH has been widely utilised for basic and applied research on genomes’ structural organisation, function and evolution. As a diagnostic technique, FISH is extensively used in clinical genetics and molecular pathology, and for single cell identification in microbiological analyses. This talk will provide an overview of modern FISH applications and ongoing developments, with particular focus on novel areas where FISH has joined forces with high-end technologies such as super-resolution microscopy and CRISPR. The role and relevance of FISH at a time when scientific advances appear to be increasingly driven by high-throughput approaches and ‘big data’ will be explored.

Year2017
ConferenceApplications of in situ hybridisation in research and disease

Related outputs

Intact Sample Analysis
Warford, A. and Volpi, E. 2019. Intact Sample Analysis. in: Warford, A. and Presneau, Nadège (ed.) Molecular Diagnostics Oxford University Press.

In vivo modeling of human neuron dynamics and Down syndrome
Real, R., Peter, M., Trabalza, A., Khan, S., Smith, M.A., Dopp, J., Barnes, S.J., Momoh, A., Strano, A., Volpi, E., Knott, G., Livesey, F.J. and De Paola, V. 2018. In vivo modeling of human neuron dynamics and Down syndrome. Science. 362 (6416), p. eaau1810. doi:10.1126/science.aau1810

Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer?
Usman, M. and Volpi, E. 2018. Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer? European Association for Cancer Research 25th Congress. Amsterdam 01 Jul 2018 European Society for Medical Oncology (ESMO). doi:10.1136/esmoopen-2018-EACR25.63

DNA damage in obesity: Initiator, promoter and predictor of cancer
Usman, M. and Volpi, E. 2018. DNA damage in obesity: Initiator, promoter and predictor of cancer. Mutation Research/Reviews in Mutation Research. 778, pp. 23-37. doi:10.1016/j.mrrev.2018.08.002

Evaluation of oxidative DNA damage, genome instability and accelerated genomic ageing in childhood obesity
Usman, M. and Volpi, E. 2017. Evaluation of oxidative DNA damage, genome instability and accelerated genomic ageing in childhood obesity. The Human Genome in Healthcare - The Genetics Society. The Royal Society 23 - 24 Nov 2017

In situ hybridization: key concepts and applications
Warford, A. and Volpi, E. 2017. In situ hybridization: key concepts and applications. in: Orchard, G.R. and Nation, B. (ed.) Histopathology Oxford Oxford University Press. pp. 298-317

Evaluating Inflammatory and Genotoxic Consequences of Adiposity in Adolescents: a Non-Invasive Approach
Usman, M. and Volpi, E. 2017. Evaluating Inflammatory and Genotoxic Consequences of Adiposity in Adolescents: a Non-Invasive Approach. The 12th International Conference and 5th Asian Congress on Environmental Mutagens. Korea 12 - 16 Nov 2017 Korean Environmental Mutagen Society.

Genome Instability in Childhood Obesity – a potential role for bariatric surgery in cancer prevention?
Usman, M. and Volpi, E. 2017. Genome Instability in Childhood Obesity – a potential role for bariatric surgery in cancer prevention? Severe Paediatric Obesity: Current Medical and Surgical Management Paradigms - A Global Perspective. London 18 Jul 2017 International Consortium of Adolescent Bariatric Surgeons (ICABS).

Using virtual reality to prepare Bioscience students for practical classes
Coleman, S.K., Volpi, E. and Smith, C.L. 2017. Using virtual reality to prepare Bioscience students for practical classes. 16th Westminster Symposium Innovate!. University of Westminster, London 22 - 22 Jun 2017

Whole genome analysis of a schistosomiasis-transmitting freshwater snail
Adema, C.M., Hillier, L.W., Jones, C.S., Loker, E.S., Knight, M., Minx, P., Oliveira, G., Raghavan, N., Shedlock, A., Rodrigues do Amaral, L., Arican-Goktas, H.D., Assis, A.G., Baba, E.H., Baron, O.L., Bayne, C.J., Bickham-Wright, U., Biggar, K.K., Blouin, M., Bonning, B.C., Botka, C., Bridger, J.M., Buckley, K.M., Buddenborg, S.K., Caldeira, R.L., Carleton, J., Carvalho, O.S., Castillo, M.G., Chalmers, I.W., Christensens, M., Clifton, S., Cosseau, C., Coustau, C., Cripps, R.M., Cuesta-Astroz, Y., Cummins, S.F., di Stephano, L., Dinguirard, N., Duval, D., Emrich, S., Feschotte, C., Feyereisen, R., FitzGerald, P., Fronick, C., Fulton, L., Galinier, R., Gave, S.G., Geusz, M., Geyer, K.K., Giraldo-Calderón, G.I., Gomes, M.D.S., Gordy, M.A., Gourbal, B., Grunau, C., Hanington, P.C., Hoffmann, K.F., Hughes, D., Humphries, J., Jackson, D.J., Jannotti-Passos, L.K., Jeremias, W.D.J., Jobling, S., Kamel, B., Kapusta, A., Kaur, S., Koene, J.M., Kohn, A.B., Lawson, D., Lawton, S.P., Liang, D., Limpanont, Y., Liu, S., Lockyer, A.E., Lovato, T.L., Ludolf, F., Magrini, V., McManus, D.P., Medina, M., Misra, M., Mitta, G., Mkoji, G.M., Montague, M.J., Montelongo, C., Moroz, L.L., Munoz-Torres, M.C., Niazi, U., Noble, L.R., Oliveira,, F.S., Pais, F.B., Papenfuss, A.T., Peace, R., Pena, J.J., Pila, E.A., Quelais, T., Raney, B.J., Rast, J.P., Rollinson, D., Rosse, I.C., Rotgans, B., Routledge, E.J., Ryan, K.M., Scholte, L.L.S., Storey, K.B., Swain, M., Tennessen, J.A., Tomlinson, C., Trujillo, D.L., Volpi, E., Walker, A.J., Wang, T., Wannaporn, I., Warren, W.C., Wu, X.J., Yoshino, T.P., Yusuf, M., Zhang, S.M., Zhao, M. and Wilson, R.K. 2017. Whole genome analysis of a schistosomiasis-transmitting freshwater snail. Nature Communications. 8, p. 15451. doi:10.1038/ncomms15451

Genome instability in childhood obesity: A conceptual framework for an assessment, intervention and monitoring programme of inflammation and DNA damage in paediatric obesity
Usman, M., Tewfik, I. and Volpi, E. 2017. Genome instability in childhood obesity: A conceptual framework for an assessment, intervention and monitoring programme of inflammation and DNA damage in paediatric obesity. International Journal of Food, Nutrition and Public Health. 9 (1), pp. 1-12.

Formamide-free Fluorescence in situ Hybridization (FISH)
Volpi, E. 2017. Formamide-free Fluorescence in situ Hybridization (FISH). in: Liehr, T. (ed.) Fluorescence in situ Hybridization (FISH) - Application Guide Berlin Springer.

Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy
Halford, S., Liew, G., Mackay, D., Sergouniotis, P., Holt, R., Broadgate, S., Volpi, E., Ocaka, L., Robson, A., Holder, G., Moore, A., Michaelides, M. and Webster, A. 2014. Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy. Ophthalmology: Journal of The American Academy of Ophthalmology. 121 (6), pp. 1174-1184. doi:10.1016%2Fj.ophtha.2013.11.042

Language impairment in a case of a complex chromosomal rearrangement with a breakpoint downstream of FOXP2
Moralli, D., Nudel, R., Chan, M.T.M., Green, C.M., Volpi, E., Benítez-Burraco, A., Newbury, D.F. and García-Bellido, P. 2015. Language impairment in a case of a complex chromosomal rearrangement with a breakpoint downstream of FOXP2 . Molecular Cytogenetics. 8 (36). doi:10.1186/s13039-015-0148-1

De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder
Babbs, C., Lloyd, D., Pagnamenta, A., Twiggs, S., Green, J., McGowan, S., Mirza, G., Naples, R., Sharma, V., Volpi, E., Buckle, V., Wall, S., Knight, S., International Molecular Genetic Study of Autism Consortium (IMGSAC), ., Parr, J. and Wilkie, A. 2014. De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder. Journal of Medical Genetics. 51 (11), pp. 737-747. doi:10.1136%2Fjmedgenet-2014-102582

Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy
Halford, S., Liew, G., Mackay, D.S., Sergouniotis, P.I., Holt, R., Broadgate, S., Volpi, E., Ocaka, L., Robson, A.G., Holder, G.E., Moore, A.T., Michaelides, M. and Webster, A.R. 2014. Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy. Ophtalmology. 121 (6), pp. 1174-1184. doi:10.1016/j.ophtha.2013.11.042

De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder
Babbs, C., Lloyd, D., Pagnamenta, A.T., Twiggs, S.R., Green, J., McGowan, S.J., Mirza, G., Naples, R., Sharma, V.P., Volpi, E., Buckle, V.J., Wall, S.A., Knight SJ; International Molecular Genetic Study of Autism Consortium (IMGSAC), Parr, J.R. and Wilkie, A.O. 2014. De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder. Journal of Medical Genetics. 51 (11), pp. 737-747. doi:10.1136/jmedgenet-2014-102582

Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y
Yusuf, M., Leung, K., Morris, K.J. and Volpi, E. 2013. Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y. Neurogenetics. 14 (1), pp. 63-70. doi:10.1007/s10048-012-0350-9

Chromosomes and nuclear organisation in ICF syndrome
Volpi, E. 2013. Chromosomes and nuclear organisation in ICF syndrome. in: Yurov, Y.B., Vorsanova, S.G. and Iourov, I.Y. (ed.) Human interphase chromosomes: biomedical aspects New York Springer. pp. 107-121

Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device
Marie, R., Pedersen, J.N., Bauer, D.L.V., Rasmussen, K.H., Yusuf, M., Volpi, E., Flyvbjerg, H., Kristensen, A. and Mir, K.U. 2013. Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device. Proceedings of the National Academy of Sciences. 110 (13), pp. 4893-4898. doi:10.1073/pnas.1214570110

High-resolution fish on DNA fibers for low-copy repeats genome architecture studies
Molina, O., Blanco, J., Anton, E., Vidal, F. and Volpi, E. 2012. High-resolution fish on DNA fibers for low-copy repeats genome architecture studies. Genomics. 100 (6), pp. 380-386. doi:10.1016/j.ygeno.2012.08.007

Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells
Mandegar, M.A., Moralli, D., Khoja, S., Cowley, S., Chan, D.Y.L., Yusuf, M., Mukherjee, S., Blundell, M.P., Volpi, E., Thrasher, A.J., James, W. and Monaco, Z.L. 2011. Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells. Human Molecular Genetics. 20 (15), pp. 2905-2913. doi:10.1093/hmg/ddr144

An improved technique for chromosomal analysis of human ES and iPS cells
Moralli, D., Yusuf, M., Mandegar, M.A., Khoja, S., Monaco, Z.L. and Volpi, E. 2011. An improved technique for chromosomal analysis of human ES and iPS cells. Stem Cell Reviews and Reports. 7 (2), pp. 471-477. doi:10.1007/s12015-010-9224-4

Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions
Yusuf, M., Bauer, D.L.V., Lipinski, D.M., MacLaren, R.E., Wade-Martins, R., Mir, K.U. and Volpi, E. 2011. Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions. BMC Biotechnology. 11, pp. 121-131. doi:10.1186/1472-6750-11-121

Altered intra-nuclear organization of heterochromatin and genes in ICF syndrome
Jefferson, A., Colella, S., Moralli, D., Wilson, N., Yusuf, M., Gimelli, G., Ragoussis, J. and Volpi, E. 2010. Altered intra-nuclear organization of heterochromatin and genes in ICF syndrome. PLoS ONE. 5 (6), p. e11364. doi:10.1371/journal.pone.0011364

Permalink - https://westminsterresearch.westminster.ac.uk/item/q1123/fluorescence-in-situ-hybridization-fish-challenges-and-opportunities-in-the-post-genomic-era


Share this
Tweet
Email