Evaluating Inflammatory and Genotoxic Consequences of Adiposity in Adolescents: a Non-Invasive Approach

Usman, M. and Volpi, E. 2017. Evaluating Inflammatory and Genotoxic Consequences of Adiposity in Adolescents: a Non-Invasive Approach. The 12th International Conference and 5th Asian Congress on Environmental Mutagens. Korea 12 - 16 Nov 2017 Korean Environmental Mutagen Society.

Title Evaluating Inflammatory and Genotoxic Consequences of Adiposity in Adolescents: a Non-Invasive Approach
AuthorsUsman, M. and Volpi, E.
TypeConference paper
Abstract

The genome holds a substantial role in maintaining optimal cellular function. There are a variety of well-established genotoxicity tests that can evaluate acquired DNA damage and chromosomal instability. These types of tests can be utilized to inform susceptibility to disorders that are strongly associated with genome damage, such as cancer. A strong correlation has become evident between obesity and over ten different types of cancers. This association may be explained by the increased systemic inflammation and over-generation of reactive oxygen species (ROS) that is characteristic in individuals who possess excess body fat. The mutagenic effects of ROS are well-known and include oxidative base modifications and DNA single and double strand breaks. Emerging evidence indicates a correlation between increased adiposity and markers of oxidative DNA damage. One such marker is 8- OH-2-deoxy Guanosine (8-OHdG), a DNA lesion that is potentially mutagenic. Findings of other biomarkers indicative of DNA strand breaks in obesity include -H2AX foci, micronuclei and comet tails. Telomere attrition marks accelerated ageing and is another phenomenon identified in obese adults and children. Adolescent obesity is expected to rise and lead to morbidity. It is concerning that recent studies have suggested obese children to not only possess a reduced life expectancy, but a pre-disposition to age related disorders, including cancer. We propose biomonitoring of the genome to inform prioritization and severity of intervention measures. A combined laboratory ‘tool-kit’ to allow non-invasive biomonitoring of ‘genome health’ in adolescents and other large epidemiological studies is being developed. This investigation integrates the well-established micronucleus assay in exfoliated buccal epithelial cells, urinary 8-OHdG, salivary telomere length and salivary C-reactive protein to provide a comprehensive assessment of inflammation, cytotoxicity, chromosomal aberrations and DNA damage in a cohort of 11-15 year olds. Correlations of these markers is explored against three different measures of adiposity - waist to hip ratio, body fat percentage and body mass index. Findings from this study aim to establish the applicability of personalized, early detection of acquired DNA damage as a pre-cancerous biomarker in obesity and other pre-pathological conditions.

Keywordsgenome health, DNA damage, cancer, reactive oxygen species (ROS), ageing, obesity, telomeres, biomarker
Year2017
ConferenceThe 12th International Conference and 5th Asian Congress on Environmental Mutagens
PublisherKorean Environmental Mutagen Society
Accepted author manuscriptAbstract for ICEM-ACEM 2017.pdf

Related outputs

In vivo modeling of human neuron dynamics and Down syndrome
Real, R., Peter, M., Trabalza, A., Khan, S., Smith, M.A., Dopp, J., Barnes, S.J., Momoh, A., Strano, A., Volpi, E., Knott, G., Livesey, F.J. and De Paola, V. 2018. In vivo modeling of human neuron dynamics and Down syndrome. Science. 362 (6416), p. eaau1810.

Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer?
Usman, M. and Volpi, E. 2018. Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer? European Association for Cancer Research 25th Congress. Amsterdam 01 Jul 2018 European Society for Medical Oncology (ESMO).

DNA damage in obesity: Initiator, promoter and predictor of cancer
Usman, M. and Volpi, E. 2018. DNA damage in obesity: Initiator, promoter and predictor of cancer. Mutation Research/Reviews in Mutation Research. 778, pp. 23-37.

Evaluation of oxidative DNA damage, genome instability and accelerated genomic ageing in childhood obesity
Usman, M. and Volpi, E. 2017. Evaluation of oxidative DNA damage, genome instability and accelerated genomic ageing in childhood obesity. The Human Genome in Healthcare - The Genetics Society. The Royal Society 23 - 24 Nov 2017

In situ hybridization: key concepts and applications
Warford, A. and Volpi, E. 2017. In situ hybridization: key concepts and applications. in: Orchard, G.R. and Nation, B. (ed.) Histopathology Oxford Oxford University Press. pp. 298-317

Genome Instability in Childhood Obesity – a potential role for bariatric surgery in cancer prevention?
Usman, M. and Volpi, E. 2017. Genome Instability in Childhood Obesity – a potential role for bariatric surgery in cancer prevention? Severe Paediatric Obesity: Current Medical and Surgical Management Paradigms - A Global Perspective. London 18 Jul 2017 International Consortium of Adolescent Bariatric Surgeons (ICABS).

Using virtual reality to prepare Bioscience students for practical classes
Coleman, S.K., Volpi, E. and Smith, C.L. 2017. Using virtual reality to prepare Bioscience students for practical classes. 16th Westminster Symposium Innovate!. University of Westminster, London 22 - 22 Jun 2017

Fluorescence in situ Hybridization (FISH): challenges and opportunities in the post-genomic era
Volpi, E. 2017. Fluorescence in situ Hybridization (FISH): challenges and opportunities in the post-genomic era. Applications of in situ hybridisation in research and disease. Cancer Research UK Cambridge Institute, Cambridge 17 - 18 May 2017

Whole genome analysis of a schistosomiasis-transmitting freshwater snail
Adema, C.M., Hillier, L.W., Jones, C.S., Loker, E.S., Knight, M., Minx, P., Oliveira, G., Raghavan, N., Shedlock, A., Rodrigues do Amaral, L., Arican-Goktas, H.D., Assis, A.G., Baba, E.H., Baron, O.L., Bayne, C.J., Bickham-Wright, U., Biggar, K.K., Blouin, M., Bonning, B.C., Botka, C., Bridger, J.M., Buckley, K.M., Buddenborg, S.K., Caldeira, R.L., Carleton, J., Carvalho, O.S., Castillo, M.G., Chalmers, I.W., Christensens, M., Clifton, S., Cosseau, C., Coustau, C., Cripps, R.M., Cuesta-Astroz, Y., Cummins, S.F., di Stephano, L., Dinguirard, N., Duval, D., Emrich, S., Feschotte, C., Feyereisen, R., FitzGerald, P., Fronick, C., Fulton, L., Galinier, R., Gave, S.G., Geusz, M., Geyer, K.K., Giraldo-Calderón, G.I., Gomes, M.D.S., Gordy, M.A., Gourbal, B., Grunau, C., Hanington, P.C., Hoffmann, K.F., Hughes, D., Humphries, J., Jackson, D.J., Jannotti-Passos, L.K., Jeremias, W.D.J., Jobling, S., Kamel, B., Kapusta, A., Kaur, S., Koene, J.M., Kohn, A.B., Lawson, D., Lawton, S.P., Liang, D., Limpanont, Y., Liu, S., Lockyer, A.E., Lovato, T.L., Ludolf, F., Magrini, V., McManus, D.P., Medina, M., Misra, M., Mitta, G., Mkoji, G.M., Montague, M.J., Montelongo, C., Moroz, L.L., Munoz-Torres, M.C., Niazi, U., Noble, L.R., Oliveira,, F.S., Pais, F.B., Papenfuss, A.T., Peace, R., Pena, J.J., Pila, E.A., Quelais, T., Raney, B.J., Rast, J.P., Rollinson, D., Rosse, I.C., Rotgans, B., Routledge, E.J., Ryan, K.M., Scholte, L.L.S., Storey, K.B., Swain, M., Tennessen, J.A., Tomlinson, C., Trujillo, D.L., Volpi, E., Walker, A.J., Wang, T., Wannaporn, I., Warren, W.C., Wu, X.J., Yoshino, T.P., Yusuf, M., Zhang, S.M., Zhao, M. and Wilson, R.K. 2017. Whole genome analysis of a schistosomiasis-transmitting freshwater snail. Nature Communications. 8, p. 15451.

Genome instability in childhood obesity: A conceptual framework for an assessment, intervention and monitoring programme of inflammation and DNA damage in paediatric obesity
Usman, M., Tewfik, I. and Volpi, E. 2017. Genome instability in childhood obesity: A conceptual framework for an assessment, intervention and monitoring programme of inflammation and DNA damage in paediatric obesity. International Journal of Food, Nutrition and Public Health. 9 (1), pp. 1-12.

Formamide-free Fluorescence in situ Hybridization (FISH)
Volpi, E. 2017. Formamide-free Fluorescence in situ Hybridization (FISH). in: Liehr, T. (ed.) Fluorescence in situ Hybridization (FISH) - Application Guide Berlin Springer.

Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy
Halford, S., Liew, G., Mackay, D., Sergouniotis, P., Holt, R., Broadgate, S., Volpi, E., Ocaka, L., Robson, A., Holder, G., Moore, A., Michaelides, M. and Webster, A. 2014. Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy. Ophthalmology: Journal of The American Academy of Ophthalmology. 121 (6), pp. 1174-1184.

Language impairment in a case of a complex chromosomal rearrangement with a breakpoint downstream of FOXP2
Moralli, D., Nudel, R., Chan, M.T.M., Green, C.M., Volpi, E., Benítez-Burraco, A., Newbury, D.F. and García-Bellido, P. 2015. Language impairment in a case of a complex chromosomal rearrangement with a breakpoint downstream of FOXP2 . Molecular Cytogenetics. 8 (36).

De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder
Babbs, C., Lloyd, D., Pagnamenta, A., Twiggs, S., Green, J., McGowan, S., Mirza, G., Naples, R., Sharma, V., Volpi, E., Buckle, V., Wall, S., Knight, S., International Molecular Genetic Study of Autism Consortium (IMGSAC), ., Parr, J. and Wilkie, A. 2014. De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder. Journal of Medical Genetics. 51 (11), pp. 737-747.

Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy
Halford, S., Liew, G., Mackay, D.S., Sergouniotis, P.I., Holt, R., Broadgate, S., Volpi, E., Ocaka, L., Robson, A.G., Holder, G.E., Moore, A.T., Michaelides, M. and Webster, A.R. 2014. Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy. Ophtalmology. 121 (6), pp. 1174-1184.

De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder
Babbs, C., Lloyd, D., Pagnamenta, A.T., Twiggs, S.R., Green, J., McGowan, S.J., Mirza, G., Naples, R., Sharma, V.P., Volpi, E., Buckle, V.J., Wall, S.A., Knight SJ; International Molecular Genetic Study of Autism Consortium (IMGSAC), Parr, J.R. and Wilkie, A.O. 2014. De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder. Journal of Medical Genetics. 51 (11), pp. 737-747.

Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y
Yusuf, M., Leung, K., Morris, K.J. and Volpi, E. 2013. Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y. Neurogenetics. 14 (1), pp. 63-70.

Chromosomes and nuclear organisation in ICF syndrome
Volpi, E. 2013. Chromosomes and nuclear organisation in ICF syndrome. in: Yurov, Y.B., Vorsanova, S.G. and Iourov, I.Y. (ed.) Human interphase chromosomes: biomedical aspects New York Springer. pp. 107-121

Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device
Marie, R., Pedersen, J.N., Bauer, D.L.V., Rasmussen, K.H., Yusuf, M., Volpi, E., Flyvbjerg, H., Kristensen, A. and Mir, K.U. 2013. Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device. Proceedings of the National Academy of Sciences. 110 (13), pp. 4893-4898.

High-resolution fish on DNA fibers for low-copy repeats genome architecture studies
Molina, O., Blanco, J., Anton, E., Vidal, F. and Volpi, E. 2012. High-resolution fish on DNA fibers for low-copy repeats genome architecture studies. Genomics. 100 (6), pp. 380-386.

Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells
Mandegar, M.A., Moralli, D., Khoja, S., Cowley, S., Chan, D.Y.L., Yusuf, M., Mukherjee, S., Blundell, M.P., Volpi, E., Thrasher, A.J., James, W. and Monaco, Z.L. 2011. Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells. Human Molecular Genetics. 20 (15), pp. 2905-2913.

An improved technique for chromosomal analysis of human ES and iPS cells
Moralli, D., Yusuf, M., Mandegar, M.A., Khoja, S., Monaco, Z.L. and Volpi, E. 2011. An improved technique for chromosomal analysis of human ES and iPS cells. Stem Cell Reviews and Reports. 7 (2), pp. 471-477.

Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions
Yusuf, M., Bauer, D.L.V., Lipinski, D.M., MacLaren, R.E., Wade-Martins, R., Mir, K.U. and Volpi, E. 2011. Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions. BMC Biotechnology. 11, pp. 121-131.

Altered intra-nuclear organization of heterochromatin and genes in ICF syndrome
Jefferson, A., Colella, S., Moralli, D., Wilson, N., Yusuf, M., Gimelli, G., Ragoussis, J. and Volpi, E. 2010. Altered intra-nuclear organization of heterochromatin and genes in ICF syndrome. PLoS ONE. 5 (6), p. e11364.

Permalink - https://westminsterresearch.westminster.ac.uk/item/q1980/-evaluating-inflammatory-and-genotoxic-consequences-of-adiposity-in-adolescents-a-non-invasive-approach


Share this
Tweet
Email