Obesity, oxidative DNA damage and vitamin D as predictors of genomic instability in children and adolescents

Usman, M., Woloshynowych, M., Britto, J.C., Bilkevic, I., Glassar, B., Chapman, S., Ford-Adams, M.E., Desai, A., Bain, M., Tewfik, I. and Volpi, E. 2021. Obesity, oxidative DNA damage and vitamin D as predictors of genomic instability in children and adolescents. International Journal of Obesity. 45, p. 2095–2107. https://doi.org/10.1038/s41366-021-00879-2

TitleObesity, oxidative DNA damage and vitamin D as predictors of genomic instability in children and adolescents
TypeJournal article
AuthorsUsman, M., Woloshynowych, M., Britto, J.C., Bilkevic, I., Glassar, B., Chapman, S., Ford-Adams, M.E., Desai, A., Bain, M., Tewfik, I. and Volpi, E.
Abstract

Background/objectives
Epidemiological evidence indicates obesity in childhood and adolescence to be an independent risk factor for cancer and premature mortality in adulthood. Pathological implications from excess adiposity may begin early in life. Obesity is concurrent with a state of chronic inflammation, a well-known aetiological factor for DNA damage. In addition, obesity has been associated with micro-nutritional deficiencies. Vitamin D has attracted attention for its anti-inflammatory properties and role in genomic integrity and stability. The aim of this study was to determine a novel approach for predicting genomic instability via the combined assessment of adiposity, DNA damage, systemic inflammation, and vitamin D status.

Subjects/methods
We carried out a cross-sectional study with 132 participants, aged 10–18, recruited from schools and paediatric obesity clinics in London. Anthropometric assessments included BMI Z-score, waist and hip circumference, and body fat percentage via bioelectrical impedance. Inflammation and vitamin D levels in saliva were assessed by enzyme-linked immunosorbent assay. Oxidative DNA damage was determined via quantification of 8-hydroxy-2′-deoxyguanosine in urine. Exfoliated cells from the oral cavity were scored for genomic instability via the buccal cytome assay.

Results
As expected, comparisons between participants with obesity and normal range BMI showed significant differences in anthropometric measures (p < 0.001). Significant differences were also observed in some measures of genomic instability (p < 0.001). When examining relationships between variables for all participants, markers of adiposity positively correlated with acquired oxidative DNA damage (p < 0.01) and genomic instability (p < 0.001), and negatively correlated with vitamin D (p < 0.01). Multiple regression analyses identified obesity (p < 0.001), vitamin D (p < 0.001), and oxidative DNA damage (p < 0.05) as the three significant predictors of genomic instability.

Conclusions
Obesity, oxidative DNA damage, and vitamin D deficiency are significant predictors of genomic instability. Non-invasive biomonitoring and predictive modelling of genomic instability in young patients with obesity may contribute to the prioritisation and severity of clinical intervention measures.

KeywordsCancer, Obesity, Risk factors
JournalInternational Journal of Obesity
Journal citation45, p. 2095–2107
ISSN1476-5497
Year2021
PublisherNature Publishing Group
Accepted author manuscript
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1038/s41366-021-00879-2
PubMed ID34158611
Web address (URL)https://www.nature.com/articles/s41366-021-00879-2
Publication dates
Published online22 Jun 2021
Supplemental file
License
CC BY 4.0
File Access Level
Open (open metadata and files)

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Hmedeh, C., Ghazeeri, G. and Tewfik, I. 2018. Nutritional management in polycystic ovary syndrome: challenges and opportunities. International Journal of Food Safety, Nutrition and Public Health. 3 (2), p. 870–82. https://doi.org/10.1504/IJFSNPH.2021.113389

PO-018 Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer?
Usman, M. and Volpi, E.V. 2018. PO-018 Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer? ESMO Open. 3 (Supplement 2), pp. A27-A28. https://doi.org/10.1136/esmoopen-2018-eacr25.66

Appraising the Effects of Physical Activity Interventions on Risk Factors of Cardiovascular Diseases and Obesity: A Systematic Review
Sum, K. and Tewfik, I. 2018. Appraising the Effects of Physical Activity Interventions on Risk Factors of Cardiovascular Diseases and Obesity: A Systematic Review. International Journal of Food, Nutrition and Public Health. 10 (1), pp. 40-77. https://doi.org/10.47556/J.IJFNPH.10.1.2018.4

In vivo modeling of human neuron dynamics and Down syndrome
Raquel Real, Manuel Peter, Antonio Trabalza, Shabana Khan, Mark Smith, Joana Dopp, Samuel Barnes, Ayiba Momoh, Alessio Strano, Volpi, E., Graham W Knott, F. J. Livesey and Vincenzo De Paola 2018. In vivo modeling of human neuron dynamics and Down syndrome. Apollo - University of Cambridge Repository. 362 (6416), p. eaau1810 eaau1810. https://doi.org/10.17863/cam.46476

Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer?
Usman, M. and Volpi, E. 2018. Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer? European Association for Cancer Research 25th Congress. Amsterdam 01 Jul 2018 European Society for Medical Oncology (ESMO). https://doi.org/10.1136/esmoopen-2018-EACR25.63

DNA damage in obesity: Initiator, promoter and predictor of cancer
Usman, M. and Volpi, E. 2018. DNA damage in obesity: Initiator, promoter and predictor of cancer. Mutation Research/Reviews in Mutation Research. 778, pp. 23-37. https://doi.org/10.1016/j.mrrev.2018.08.002

Evaluation of a photographic food atlas as a tool for quantifying food portion size in the United Arab Emirates
Ali, H., Platat, C., El Mesmoudi, N., El Sadig, M. and Tewfik, I. 2018. Evaluation of a photographic food atlas as a tool for quantifying food portion size in the United Arab Emirates. PLoS ONE. 13 (4) e0196389. https://doi.org/10.1371/journal.pone.0196389

Evaluation of plasma neurotransmitters in children living with Attention-Deficit Hyperactivity Disorder (ADHD)
Meguid, N., Anwar, M., Hussein, J., Hasan, H., Hashish, A., Seleet, F., Foda, M. and Tewfik, I. 2018. Evaluation of plasma neurotransmitters in children living with Attention-Deficit Hyperactivity Disorder (ADHD). Bioscience Research. 15 (1), pp. 152-159.

Dietary fibre regimen to modulate weight management: a pilot intervention
Khawandanah, J. and Tewfik, I. 2017. Dietary fibre regimen to modulate weight management: a pilot intervention. International Journal of Food, Nutrition and Public Health. 9 (2), pp. 67-79.

Evaluation of oxidative DNA damage, genome instability and accelerated genomic ageing in childhood obesity
Usman, M. and Volpi, E. 2017. Evaluation of oxidative DNA damage, genome instability and accelerated genomic ageing in childhood obesity. The Human Genome in Healthcare - The Genetics Society. The Royal Society 23 - 24 Nov 2017

In situ hybridization: key concepts and applications
Warford, A. and Volpi, E. 2017. In situ hybridization: key concepts and applications. in: Orchard, G.R. and Nation, B. (ed.) Histopathology Oxford Oxford University Press. pp. 298-317

Evaluating Inflammatory and Genotoxic Consequences of Adiposity in Adolescents: a Non-Invasive Approach
Usman, M. and Volpi, E. 2017. Evaluating Inflammatory and Genotoxic Consequences of Adiposity in Adolescents: a Non-Invasive Approach. The 12th International Conference and 5th Asian Congress on Environmental Mutagens. Korea 12 - 16 Nov 2017 Korean Environmental Mutagen Society.

Genome Instability in Childhood Obesity – a potential role for bariatric surgery in cancer prevention?
Usman, M. and Volpi, E. 2017. Genome Instability in Childhood Obesity – a potential role for bariatric surgery in cancer prevention? Severe Paediatric Obesity: Current Medical and Surgical Management Paradigms - A Global Perspective. London 18 Jul 2017 International Consortium of Adolescent Bariatric Surgeons (ICABS).

Using virtual reality to prepare Bioscience students for practical classes
Coleman, S.K., Volpi, E. and Smith, C.L. 2017. Using virtual reality to prepare Bioscience students for practical classes. 16th Westminster Symposium Innovate!. University of Westminster, London 22 - 22 Jun 2017

Fluorescence in situ Hybridization (FISH): challenges and opportunities in the post-genomic era
Volpi, E. 2017. Fluorescence in situ Hybridization (FISH): challenges and opportunities in the post-genomic era. Applications of in situ hybridisation in research and disease. Cancer Research UK Cambridge Institute, Cambridge 17 - 18 May 2017

Whole genome analysis of a schistosomiasis-transmitting freshwater snail
Adema, C.M., Hillier, L.W., Jones, C.S., Loker, E.S., Knight, M., Minx, P., Oliveira, G., Raghavan, N., Shedlock, A., Rodrigues do Amaral, L., Arican-Goktas, H.D., Assis, A.G., Baba, E.H., Baron, O.L., Bayne, C.J., Bickham-Wright, U., Biggar, K.K., Blouin, M., Bonning, B.C., Botka, C., Bridger, J.M., Buckley, K.M., Buddenborg, S.K., Caldeira, R.L., Carleton, J., Carvalho, O.S., Castillo, M.G., Chalmers, I.W., Christensens, M., Clifton, S., Cosseau, C., Coustau, C., Cripps, R.M., Cuesta-Astroz, Y., Cummins, S.F., di Stephano, L., Dinguirard, N., Duval, D., Emrich, S., Feschotte, C., Feyereisen, R., FitzGerald, P., Fronick, C., Fulton, L., Galinier, R., Gave, S.G., Geusz, M., Geyer, K.K., Giraldo-Calderón, G.I., Gomes, M.D.S., Gordy, M.A., Gourbal, B., Grunau, C., Hanington, P.C., Hoffmann, K.F., Hughes, D., Humphries, J., Jackson, D.J., Jannotti-Passos, L.K., Jeremias, W.D.J., Jobling, S., Kamel, B., Kapusta, A., Kaur, S., Koene, J.M., Kohn, A.B., Lawson, D., Lawton, S.P., Liang, D., Limpanont, Y., Liu, S., Lockyer, A.E., Lovato, T.L., Ludolf, F., Magrini, V., McManus, D.P., Medina, M., Misra, M., Mitta, G., Mkoji, G.M., Montague, M.J., Montelongo, C., Moroz, L.L., Munoz-Torres, M.C., Niazi, U., Noble, L.R., Oliveira,, F.S., Pais, F.B., Papenfuss, A.T., Peace, R., Pena, J.J., Pila, E.A., Quelais, T., Raney, B.J., Rast, J.P., Rollinson, D., Rosse, I.C., Rotgans, B., Routledge, E.J., Ryan, K.M., Scholte, L.L.S., Storey, K.B., Swain, M., Tennessen, J.A., Tomlinson, C., Trujillo, D.L., Volpi, E., Walker, A.J., Wang, T., Wannaporn, I., Warren, W.C., Wu, X.J., Yoshino, T.P., Yusuf, M., Zhang, S.M., Zhao, M. and Wilson, R.K. 2017. Whole genome analysis of a schistosomiasis-transmitting freshwater snail. Nature Communications. 8, p. 15451 15451. https://doi.org/10.1038/ncomms15451

Genome instability in childhood obesity: A conceptual framework for an assessment, intervention and monitoring programme of inflammation and DNA damage in paediatric obesity
Usman, M., Tewfik, I. and Volpi, E. 2017. Genome instability in childhood obesity: A conceptual framework for an assessment, intervention and monitoring programme of inflammation and DNA damage in paediatric obesity. International Journal of Food, Nutrition and Public Health. 9 (1), pp. 1-12.

Formamide-free Fluorescence in situ Hybridization (FISH)
Volpi, E. 2017. Formamide-free Fluorescence in situ Hybridization (FISH). in: Liehr, T. (ed.) Fluorescence in situ Hybridization (FISH) - Application Guide Berlin Springer.

A Prospective Randomized Controlled Trial to Study the Impact of a Nutrition-Sensitive Intervention on Adult Women With Cancer Cachexia Undergoing Palliative Care in India
Kapoor, N., Naufahu, J., Tewfik, S., Bhatnagar, S., Garg, R. and Tewfik, I. 2017. A Prospective Randomized Controlled Trial to Study the Impact of a Nutrition-Sensitive Intervention on Adult Women With Cancer Cachexia Undergoing Palliative Care in India. Integrative Cancer Therapies. 6 (1), pp. 74-84. https://doi.org/10.1177/1534735416651968

Fad Diets: Lifestyle Promises and Health Challenges
Khawandanah, Jomana and Tewfik, Ihab 2016. Fad Diets: Lifestyle Promises and Health Challenges. Journal of Food Research. 5 (6), pp. 80-94. https://doi.org/10.5539/jfr.v5n6p80

Palliative Nutritional Care for Cancer Patients
Kapoor, Neha, Garg, Rakesh and Tewfik, Ihab 2016. Palliative Nutritional Care for Cancer Patients. Palliative Medicine and Hospice Care. 1 (3), pp. e1-e6. https://doi.org/10.17140/PMHCOJ1-e002

Work conditions, mental workload, and patient care quality: a multisource study in the emergency department.
Weigl, M., Müller, A., Holland, S., Wedel, S. and Woloshynowych, M. 2016. Work conditions, mental workload, and patient care quality: a multisource study in the emergency department. BMJ Quality and Safety. 25, pp. 499-508. https://doi.org/10.1136/bmjqs-2014-003744

Risk Factors for Maternal Vitamin D Deficiency within the United Arab Emirates
Hussein, I., Taha, Z., Tewfik, I., Badawi, S., Siddieg, H., Adegboye, A.R. and McGrady, K. 2016. Risk Factors for Maternal Vitamin D Deficiency within the United Arab Emirates. Journal of Pregnancy and Child Health. 3 (5), p. 1000276 1000276. https://doi.org/10.4172/2376-127X.1000276

A comparative study: long and short term effect of a nutrition sensitive approach to delay the progression of HIV to AIDS among people living with HIV (PLWH) in Nigeria
Amlogu, A., Tewfik, S., Wambebe, C. and Tewfik, I. 2016. A comparative study: long and short term effect of a nutrition sensitive approach to delay the progression of HIV to AIDS among people living with HIV (PLWH) in Nigeria. Functional Foods in Health and Disease. 6 (2), pp. 79-90.

Dietary Patterns of Children with Autism Spectrum Disorder: A Study Based in Egypt
Tewfik, I. 2015. Dietary Patterns of Children with Autism Spectrum Disorder: A Study Based in Egypt. Open Access Macedonian Journal of Medical Sciences. 3 (2), pp. 262-267. https://doi.org/10.3889/oamjms.2015.051

Language impairment in a case of a complex chromosomal rearrangement with a breakpoint downstream of FOXP2
Moralli, D., Nudel, R., Chan, M.T.M., Green, C.M., Volpi, E., Benítez-Burraco, A., Newbury, D.F. and García-Bellido, P. 2015. Language impairment in a case of a complex chromosomal rearrangement with a breakpoint downstream of FOXP2 . Molecular Cytogenetics. 8 36. https://doi.org/10.1186/s13039-015-0148-1

A public health nutrition intervention to delay the progression of cachexia to refractory cachexia in Indian female cancer patients: A conceptual framework
Tewfik, I. 2014. A public health nutrition intervention to delay the progression of cachexia to refractory cachexia in Indian female cancer patients: A conceptual framework. International Journal of Food, Nutrition and Public Health. 7 (1).

Therapeutic antioxidant activity of monascus-fermented durian seed: a potential functional food ingredient, Ignatius Srianta, Ira Nugerahani, Netty Kusumawati, Elisabet Suryatanijaya, Christine Subianto, Sundus Tewfik and Ihab Tewfik
Srianta I., Nugerahani, I., Kusumawati, N., Suryatanijaya, E., Subianto, C., Tewfik S. and Tewfik, I. 2014. Therapeutic antioxidant activity of monascus-fermented durian seed: a potential functional food ingredient, Ignatius Srianta, Ira Nugerahani, Netty Kusumawati, Elisabet Suryatanijaya, Christine Subianto, Sundus Tewfik and Ihab Tewfik. International Journal of Food, Nutrition and Public Health. 7 (1).

Physical activity delivered in workplace: A pilot study to assess the health outcomes among employees at work
Hammarberg, E., Papp, R., Gaywood, A., Ferraroli, J. and Tewfik, I. 2014. Physical activity delivered in workplace: A pilot study to assess the health outcomes among employees at work. International Journal of Food, Nutrition and Public Health. 7 (1), pp. 41-52.

Tailored Functional Recipe (TFR) Approach to Delay the Progression of HIV to AIDS among People Living with HIV (PLWH) in Abuja, Nigeria
Amlogu A.M., Tewfik S., Wambebe C., Godden K. and Tewfik, I. 2014. Tailored Functional Recipe (TFR) Approach to Delay the Progression of HIV to AIDS among People Living with HIV (PLWH) in Abuja, Nigeria. Pharmacology & Pharmacy. 5 (10), pp. 926-936. https://doi.org/10.4236/pp.2014.510104

The impact of tailored nutrition intervention and dietary counseling on body weight in female cancer cachexia patients in India
Kapoor N., Naufahu, J., Tewfik, S., Bhatnagar, S., Gargand, R. and Tewfik, I. 2014. The impact of tailored nutrition intervention and dietary counseling on body weight in female cancer cachexia patients in India. Journal of Clinical Oncology. 32 (31), p. 67.

Nanofluidics to Enhance Single Molecule DNA Imaging: Detecting Genomic Structural Variation in Humans
David L.V. Bauer, Rodolphe M. Marie, Jonas N. Pedersen, Kristian H. Rasmussen, Mohammed Yusuf, Emanuela Volpi, Henrik Flyvbjerg, Anders Kristensen and Kalim U. Mir 2014. Nanofluidics to Enhance Single Molecule DNA Imaging: Detecting Genomic Structural Variation in Humans. Biophysical Journal. 106 (2, supplement 1), p. 395A. https://doi.org/10.1016/j.bpj.2013.11.2229

Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy
Halford, S., Liew, G., Mackay, D.S., Sergouniotis, P.I., Holt, R., Broadgate, S., Volpi, E., Ocaka, L., Robson, A.G., Holder, G.E., Moore, A.T., Michaelides, M. and Webster, A.R. 2014. Detailed phenotypic and genotypic characterization of bietti crystalline dystrophy. Ophtalmology. 121 (6), pp. 1174-1184. https://doi.org/10.1016/j.ophtha.2013.11.042

De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder
Babbs, C., Lloyd, D., Pagnamenta, A.T., Twiggs, S.R., Green, J., McGowan, S.J., Mirza, G., Naples, R., Sharma, V.P., Volpi, E., Buckle, V.J., Wall, S.A., Knight SJ; International Molecular Genetic Study of Autism Consortium (IMGSAC), Parr, J.R. and Wilkie, A.O. 2014. De novo and rare inherited mutations implicate the transcriptional coregulator TCF20/SPBP in autism spectrum disorder. Journal of Medical Genetics. 51 (11), pp. 737-747. https://doi.org/10.1136/jmedgenet-2014-102582

American skullcap (Scutellaria lateriflora): a randomised, double-blind placebo-controlled crossover study of its effects on mood in healthy volunteers
Brock, C., Whitehouse, J., Tewfik, I. and Towell, A. 2014. American skullcap (Scutellaria lateriflora): a randomised, double-blind placebo-controlled crossover study of its effects on mood in healthy volunteers. Phytotherapy Research. 28 (5), pp. 692-698. https://doi.org/10.1002/ptr.5044

Identity issues surrounding American skullcap (Scutellaria lateriflora) and an optimised high performance liquid chromatography method to authenticate commercially available products
Brock, C., Whitehouse, J., Tewfik, I. and Towell, A. 2013. Identity issues surrounding American skullcap (Scutellaria lateriflora) and an optimised high performance liquid chromatography method to authenticate commercially available products. Journal of Herbal Medicine. 3 (2), pp. 57-64. https://doi.org/10.1016/j.hermed.2013.02.001

Public Health Nutrition Intervention Programme to Attenuate the Progression of HIV to AIDS among People Living with HIV (PLWH) in Abuja, Nigeria: A Conceptual Framework
Amlogu, A.M., Godden, K. and Tewfik, I. 2013. Public Health Nutrition Intervention Programme to Attenuate the Progression of HIV to AIDS among People Living with HIV (PLWH) in Abuja, Nigeria: A Conceptual Framework. International Journal of Food, Nutrition and Public Health. 6 (1), pp. 83-98.

The impact of dietary and lifestyle factors on the risk of dental caries among young children in Qatar
Bener, A., Al Darwish, Mohammed S., Tewfik, I. and Hoffmann, Georg F. 2013. The impact of dietary and lifestyle factors on the risk of dental caries among young children in Qatar. Journal of the Egyptian Public Health Association. 88 (2), pp. 67-73. https://doi.org/10.1097/01.EPX.0000430962.70261.8e

A multicentre observational study to evaluate a new tool to assess emergency physicians' non-technical skills
Flowerdew, L., Gaunt, A., Spedding, J., Bhargava, A., Brown, R., Vincent, C. and Woloshynowych, M. 2013. A multicentre observational study to evaluate a new tool to assess emergency physicians' non-technical skills. Emergency Medicine Journal. 30 (6), pp. 437-443. https://doi.org/10.1136/emermed-2012-201237

Comparative study of artificial chromosome centromeres in human and murine cells
Moralli, D., Jefferson, A., Volpi, E. and Larin Monaco, Z. 2013. Comparative study of artificial chromosome centromeres in human and murine cells. European Journal of Human Genetics. 21, pp. 948-956. https://doi.org/10.1038/ejhg.2012.296

Fully Streched Single DNA Molecules in a Nanofluidic Chip Show Large-Scale Structural Variation
Jonas N. Pedersen, Rodolphe Marie, David L.V. Bauer, Kristian H. Rasmussen, Mohammed Yusuf, Emanuela V. Volpi, Anders Kristensen, Kalim U. Mir and Henrik Flyvbjerg 2013. Fully Streched Single DNA Molecules in a Nanofluidic Chip Show Large-Scale Structural Variation. Biophysical Journal. 104 (2, Supplement 1,), p. 175A. https://doi.org/10.1016/j.bpj.2012.11.986

Chromosomes and nuclear organisation in ICF syndrome
Volpi, E. 2013. Chromosomes and nuclear organisation in ICF syndrome. in: Yurov, Y.B., Vorsanova, S.G. and Iourov, I.Y. (ed.) Human interphase chromosomes: biomedical aspects New York Springer. pp. 107-121

Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device.
Marie, R., Pedersen, J.N., Bauer, D.L., Rasmussen, K.H., Yusuf, M., Volpi, E., Flyvbjerg, H., Kristensen, A. and Mir, K.U. 2013. Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device. Proceedings of the National Academy of Sciences of the United States of America. 110 (13), pp. 4893-4898. https://doi.org/10.1073/pnas.1214570110

Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y
Yusuf, M., Leung, K., Morris, K.J. and Volpi, E. 2013. Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y. Neurogenetics. 14 (1), pp. 63-70. https://doi.org/10.1007/s10048-012-0350-9

Hepatic mitochondrial dysfunction induced by fatty acids and ethanol
Gyamfi, D., Everitt, H.E., Tewfik, I., Clemens, D.L. and Patel, V. 2012. Hepatic mitochondrial dysfunction induced by fatty acids and ethanol. Free Radical Biology & Medicine. 53 (11), pp. 2131-2145. https://doi.org/10.1016/j.freeradbiomed.2012.09.024

The use of Scutellaria lateriflora: a pilot survey amongst herbal medicine practitioners
Brock, C., Whitehouse, J., Tewfik, I. and Towell, A. 2012. The use of Scutellaria lateriflora: a pilot survey amongst herbal medicine practitioners. Journal of Herbal Medicine. 2 (2), pp. 34-41. https://doi.org/10.1016/j.hermed.2012.04.005,

Tailored food recipes-TFR: employing the European perspective
Amlogu, A.M., Godden, K., Tewfik, S., Wambebe, C. & and Tewfik, I. 2012. Tailored food recipes-TFR: employing the European perspective. International Journal of Food, Nutrition and Public Health. 5 (1/2/3), pp. 3-18.

Teams under pressure in the emergency department: an interview study
Flowerdew, L., Brown, R., Vincent, C. and Woloshynowych, M. 2012. Teams under pressure in the emergency department: an interview study. Emergency Medicine Journal. 29 e2. https://doi.org/10.1136/emermed-2011-200084

Identifying Nontechnical Skills Associated With Safety in the Emergency Department: A Scoping Review of the Literature
Flowerdew, L., Brown, R., Vincent, C. and Woloshynowych, M. 2012. Identifying Nontechnical Skills Associated With Safety in the Emergency Department: A Scoping Review of the Literature. Annals of Emergency Medicine. 59 (5), pp. 386-395. https://doi.org/10.1016/j.annemergmed.2011.11.021

Development and Validation of a Tool to Assess Emergency Physicians' Nontechnical Skills
Flowerdew, L., Brown, R., Vincent, C. and Woloshynowych, M. 2012. Development and Validation of a Tool to Assess Emergency Physicians' Nontechnical Skills. Annals of Emergency Medicine. 59 (5), pp. 376-385. https://doi.org/10.1016/j.annemergmed.2011.11.022

Structures of lysenin reveal a shared evolutionary origin for pore-forming proteins and its mode of sphingomyelin recognition
De Colibus, L., Sonnen, A.F.-P., Morris, K.J., Siebert, C.A., Abrusci, P., Plitzko, J., Hodnik, V., Leippe, M., Volpi, E., Anderluh, G. and Gilbert, R.J.C. 2012. Structures of lysenin reveal a shared evolutionary origin for pore-forming proteins and its mode of sphingomyelin recognition. Structure. 20 (9), pp. P1498-1507. https://doi.org/10.1016/j.str.2012.06.011

The ABC of Handover: Impact on shift handover in the Emergency Department
Farhan, M., Brown, R., Vincent, C. and Woloshynowych, M. 2012. The ABC of Handover: Impact on shift handover in the Emergency Department. Emergency Medicine Journal. 19, pp. 947-953. https://doi.org/10.1136/emermed-2011-200201

The ABC of handover: a qualitative study to develop a new tool for handover in the emergency department.
Farhan, M., Brown, R., Woloshynowych, M. and Vincent, C. 2012. The ABC of handover: a qualitative study to develop a new tool for handover in the emergency department. Emergency Medicine Journal. 29, pp. 941-946. https://doi.org/10.1136/emermed-2011-200199

High-resolution fish on DNA fibers for low-copy repeats genome architecture studies.
Molina, O., Blanco, J., Anton, E., Vidal, F. and Volpi, E.V. 2012. High-resolution fish on DNA fibers for low-copy repeats genome architecture studies. Genomics. 100 (6), pp. 380-386. https://doi.org/10.1016/j.ygeno.2012.08.007

Obesity and low vision as a result of excessive internet use, and television viewing
Bener, A., Al-Mahdi, H.S., Ali, A.I., Al-Nufal, M., Vachhani, P.J. and Tewfik, I. 2011. Obesity and low vision as a result of excessive internet use, and television viewing. International Journal of Food Sciences and Nutrition. 62 (1), pp. 60-62. https://doi.org/10.3109/09637486.2010.495711

Artificial trans fatty acids in food supply: A global health threat
Salomaa V.K. and Tewfik, I. 2011. Artificial trans fatty acids in food supply: A global health threat. World Journal of Science, Technology and Sustainable Development. 8 (4), pp. 361-401. https://doi.org/10.1108/20425945201100017

Patient safety factors in children dying in a paediatric intensive care unit (PICU): a case notes review study
Monroe, K., Wang, D., Vincent, C., Woloshynowych, M., Neale, G. and Inwald, D.P. 2011. Patient safety factors in children dying in a paediatric intensive care unit (PICU): a case notes review study. BMJ Quality and Safety. 20, pp. 863-868. https://doi.org/10.1136/bmjqs.2010.048504

A family with autism and rare copy number variants disrupting the Duchenne/Becker muscular dystrophy gene DMD and TRPM3
Pagnamenta, A.T., Holt, R., Yusuf, M., Pinto, D., Wing, K., Betancur, C., Scherer, S.W., Volpi, E.V. and Monaco, A.P. 2011. A family with autism and rare copy number variants disrupting the Duchenne/Becker muscular dystrophy gene DMD and TRPM3. Journal of Neurodevelopmental Disorders. 3, pp. 124-131. https://doi.org/10.1007/s11689-011-9076-5

Characterization of a dominant cone degeneration in a green fluorescent protein-reporter mouse with disruption of Loci associated with human dominant retinal dystrophy.
Lipinski, D.M., Yusuf, M., Barnard, A.R., Damant, C., Charbel Issa, P., Singh, M.S., Lee, E., Davies, W.L., Volpi, E.V. and MacLaren R.E. 2011. Characterization of a dominant cone degeneration in a green fluorescent protein-reporter mouse with disruption of Loci associated with human dominant retinal dystrophy. Investigative Ophthalmology & Visual Science. 52 (9), pp. 6617-6623. https://doi.org/10.1167/iovs.11-7932

Perforin activity at membranes leads to invaginations and vesicle formation
Praper, T., Sonnen, A.F., Kladnik, A., Andrighetti, A.O., Viero, G., Morris, K.J., Volpi, E.V., Lunelli, L., Dalla Serra, M., Froelich, C.J., Gilbert, R.J. and Anderluh, G. 2011. Perforin activity at membranes leads to invaginations and vesicle formation. Proceedings of the National Academy of Sciences of the United States of America. 108 (52). https://doi.org/10.1073/pnas.1107473108

Stress Management Training for Surgeons - a randomised controlled intervention study
Wetzel, C.M., George, A., Hanna, G.B., Athanasiou, T., Black, S.A., Kneebone, R.L., Nestel, D. and Woloshynowych, M. 2011. Stress Management Training for Surgeons - a randomised controlled intervention study. Annals of Surgery. 253 (3), pp. 488-494. https://doi.org/10.1097/SLA.0b013e318209a594

Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions
Yusuf, M., Bauer, D.L.V., Lipinski, D.M., MacLaren, R.E., Wade-Martins, R., Mir, K.U. and Volpi, E. 2011. Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions. BMC Biotechnology. 11, pp. 121-131. https://doi.org/10.1186/1472-6750-11-121

Globesity in action: systematic review and pilot intervention to assess the potential health benefit of moderate physical activity
Salah, M. and Tewfik, I. 2011. Globesity in action: systematic review and pilot intervention to assess the potential health benefit of moderate physical activity. in: Ahmed, A. and Busler, M. (ed.) World sustainable development outlook 2011. Sharing knowledge, making a difference: the role of international scientific cooperation World Association for Sustainable Development (WASD).

Conceptual framework of public health-nutrition intervention programme to attenuate the progression of HIV to AIDS among people living with HIV (PLWH) in Abuja, Nigeria
Amlogu, M.A., Tewfik, S., Wambebe, C., Godden, K. and Tewfik, I. 2011. Conceptual framework of public health-nutrition intervention programme to attenuate the progression of HIV to AIDS among people living with HIV (PLWH) in Abuja, Nigeria. in: Ahmed, A. and Busler, M. (ed.) World sustainable development outlook 2011. Sharing knowledge, making a difference: the role of international scientific cooperation World Association for Sustainable Development (WASD). pp. 11-20

An improved technique for chromosomal analysis of human ES and iPS cells.
Moralli, D., Yusuf, M., Mandegar, M.A., Khoja, S., Monaco, Z.L. and Volpi, E.V. 2011. An improved technique for chromosomal analysis of human ES and iPS cells. Stem Cell Reviews. 7 (2), pp. 471-477. https://doi.org/10.1007/s12015-010-9224-4

Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells
Mandegar, M.A., Moralli, D., Khoja, S., Cowley, S., Chan, D.Y.L., Yusuf, M., Mukherjee, S., Blundell, M.P., Volpi, E., Thrasher, A.J., James, W. and Monaco, Z.L. 2011. Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells. Human Molecular Genetics. 20 (15), pp. 2905-2913. https://doi.org/10.1093/hmg/ddr144

American skullcap (Scutellaria lateriflora): an ancient remedy for today’s anxiety?
Brock, C., Whitehouse, J., Tewfik, I. and Towell, A. 2010. American skullcap (Scutellaria lateriflora): an ancient remedy for today’s anxiety? British Journal of Wellbeing. 1 (4), pp. 25-30.

Factors compromising safety in surgery: stressful events in the operating room
Arora, S., Hull, L., Tierney, T., Nestel, D., Woloshynowych, M., Darzi, A. and Kneebone, R. 2010. Factors compromising safety in surgery: stressful events in the operating room. The American Journal of Surgery. 199 (1), pp. P60-65. https://doi.org/10.1016/j.amjsurg.2009.07.036

The Imperial Stress Assessment Tool (ISAT): A feasible, reliable and valid approach to measuring stress in the Operating Room
Arora, S., Tierney, T., Sevdalis, N., Aggarwal, R., Woloshynowych, M., Nestel, D., Darzi, A. and Kneebone, R. 2010. The Imperial Stress Assessment Tool (ISAT): A feasible, reliable and valid approach to measuring stress in the Operating Room. World Journal of Surgery. 34, pp. 1756-1763. https://doi.org/10.1007/s00268-010-0559-4

The impact of stress on surgical performance: A systematic review of the literature
Arora, S., Sevdalis, N., Nestel, D., Woloshynowych, M., Darzi, A and Kneebone, R 2010. The impact of stress on surgical performance: A systematic review of the literature. Surgery. 147 (3), pp. 318-330. https://doi.org/10.1016/j.surg.2009.10.007

Reflective analysis of safety research in the hospital accident & emergency departments
Wears, R.L., Woloshynowych, M., Brown, R. and Vincent, C.A. 2010. Reflective analysis of safety research in the hospital accident & emergency departments. Applied Ergonomics. 41 (5), pp. 659-700. https://doi.org/10.1016/j.apergo.2009.12.006

Fluorescence in situ hybridization (FISH) for genomic investigations in rat
Jefferson, A. and Volpi, E.V. 2010. Fluorescence in situ hybridization (FISH) for genomic investigations in rat. in: Bridger, J. and Volpi, E. (ed.) Fluorescence in situ Hybridization (FISH). Methods in Molecular Biology (Methods and Protocols), vol 659 Humana Press. pp. 409-426

A novel three-dimensional culture system allows prolonged culture of functional human granulosa cells and mimics the ovarian environment.
Kossowska-Tomaszczuk, K., Pelczar, P., Güven, S., Kowalski, J., Volpi, E., De Geyter, C. and Scherberich, A. 2010. A novel three-dimensional culture system allows prolonged culture of functional human granulosa cells and mimics the ovarian environment. Tissue Engineering Part A. 16 (6), pp. 2063-73. https://doi.org/10.1089/ten.tea.2009.0684

Expression profiling in progressive stages of fumarate-hydratase deficiency: the contribution of metabolic changes to tumorigenesis.
Ashrafian, H., O'Flaherty, L., Adam, J., Steeples, V., Chung, Y.L., East, P., Vanharanta, S., Lehtonen, H., Nye, E., Hatipoglu, E., Miranda, M., Howarth, K., Shukla, D., Pollard, P.J. and Volpi, E. 2010. Expression profiling in progressive stages of fumarate-hydratase deficiency: the contribution of metabolic changes to tumorigenesis. Cancer Research. 70 (22), pp. 9153-9165. https://doi.org/10.1158/0008-5472.can-10-1949

The effect of stress and coping on surgical performance during simulations
Wetzel, C.M., Black, S.A., Hanna, G.B., Athanasiou, T., Kneebone, R.L., Nestel, D., Wolfe, J.H.N. and Woloshynowych, M. 2010. The effect of stress and coping on surgical performance during simulations . Annals of Surgery. 251 (1), pp. 171-176. https://doi.org/10.1097/SLA.0b013e3181b3b2be

Is Africa facing a nutrition transition under the double burden of disease?
Tewfik, I., Bener, A. and Tewfik, S. 2010. Is Africa facing a nutrition transition under the double burden of disease? in: Ahmed, A. and Nwankwo, S. (ed.) Achieving sustainable development in Africa: science, technology & innovation trajectory London World Association for Sustainable Development (WASD).

Altered intra-nuclear organisation of heterochromatin and genes in ICF syndrome.
Jefferson, A., Colella, S., Moralli, D., Wilson, N., Yusuf, M., Gimelli, G., Ragoussis, J. and Volpi, E.V. 2010. Altered intra-nuclear organisation of heterochromatin and genes in ICF syndrome. PLoS ONE. 5 (6) e11364. https://doi.org/10.1371/journal.pone.0011364

Managing Intra-operative stress: What do surgeons want from a crisis training programme?
Arora, S., Sevdalis, N., Nestel, D., Tierney, T., Woloshynowych, M. and Kneebone, R. 2009. Managing Intra-operative stress: What do surgeons want from a crisis training programme? The American Journal of Surgery. 197 (4), pp. 537-543. https://doi.org/10.1016/j.amjsurg.2008.02.009

The Care Homes Use of Medicines Study: prevalence, causes and potential harm of medication errors in care homes for older people
Barber, N.D., Alldred, D.P., Raynor, D.K., Dickinson, R., Garfield, S., Jesson, B., Lim, R., Savage, I., Standage, C., Buckle, P., Carpenter, J., Franklin, B., Woloshynowych, M. and Zermansky, A.G. 2009. The Care Homes Use of Medicines Study: prevalence, causes and potential harm of medication errors in care homes for older people. BMJ Quality and Safety. 18, pp. 341-346. https://doi.org/10.1136/qshc.2009.034231

Methodological variability in detecting prescribing errors when evaluating computerised physician order entry
Dean Franklin, B., Birch, S., Savage, I., Wong, I., Woloshynowych, M., Jacklin, A. and Barber, N. 2009. Methodological variability in detecting prescribing errors when evaluating computerised physician order entry. Pharmacoepidemiology and Drug Safety. 18 (11), pp. 992-999. https://doi.org/10.1002/pds.1811

Mapping of partially overlapping de novo deletions across an autism susceptibility region (AUTS5) in two unrelated individuals affected by developmental delays with communication impairment.
Newbury, D.F., Warburton, P.C., Wilson, N., Bacchelli, E., Carone, S., International Molecular Genetic Study of Autism Consortium, Lamb, J.A., Maestrini, E., Monaco, A.P. and Volpi, E. 2009. Mapping of partially overlapping de novo deletions across an autism susceptibility region (AUTS5) in two unrelated individuals affected by developmental delays with communication impairment. American Journal of Medical Genetics Part A. 149A (4), pp. 588-597. https://doi.org/10.1002/ajmg.a.32704

HAC stability in murine cells is influenced by nuclear localization and chromatin organization.
Moralli, D., Chan, D.Y., Jefferson, A., Volpi, E.V. and Monaco, Z.L. 2009. HAC stability in murine cells is influenced by nuclear localization and chromatin organization. BMC Cell Biology. 10 18. https://doi.org/10.1186/1471-2121-10-18

Copy number variation and association analysis of SHANK3 as a candidate gene for autism in the IMGSAC collection.
Sykes, N.H., Toma, C., Wilson, N., Volpi, E., Sousa, I., Pagnamenta, A.T., Tancredi, R., Battaglia, A., Maestrini, E., Bailey, A.J., Monaco, A.P. and International Molecular Genetic Study of Autism Consortium (IMGSAC) 2009. Copy number variation and association analysis of SHANK3 as a candidate gene for autism in the IMGSAC collection. European Journal of Human Genetics. 17, pp. 1347-1353. https://doi.org/10.1038/ejhg.2009.47

Severe insulin resistance and intrauterine growth deficiency associated with haploin sufficiency for INSR and CHN2: new insights into synergistic pathways involved in growth and metabolism
Suliman, S.G., Stanik, J., McCulloch, L.J., Wilson, N., Edghill, E.L., Misovicova, N., Gasperikova, D., Sandrikova, V., Elliott, K.S., Barak, L., Ellard, S., Volpi, E.V., Klimes, I. and Gloyn, A.L. 2009. Severe insulin resistance and intrauterine growth deficiency associated with haploin sufficiency for INSR and CHN2: new insights into synergistic pathways involved in growth and metabolism. Diabetes. 58 (12), pp. 2954-2961. https://doi.org/10.2337/db09-0787

Childhood obesity prevention in Emirates (COPE): a conceptual framework for pilot intervention
Tewfik, I., Mackenzie, J., Cunliffe, A., Al-Dhaheri, A., Ali, H., Washi, S. and Platat, C. 2009. Childhood obesity prevention in Emirates (COPE): a conceptual framework for pilot intervention. in: Ahmed, A. (ed.) World sustainable development outlook: the impact of the global financial crisis on the environment, energy and sustainable development 2009 London World Association for Sustainable Development (WASD).

Nutritional quality of deep fried street-vended foods: a public health concern
Tewfik, I., Ismail, H. and Tewfik, S. 2009. Nutritional quality of deep fried street-vended foods: a public health concern. in: Ahmed, A. (ed.) World sustainable development outlook: the impact of the global financial crisis on the environment, energy and sustainable development 2009 London World Association for Sustainable Development (WASD).

Hepatic oxidative stress and apoptosis following acute alcohol
Everitt, H.E., Tewfik, I., Preedy, V.R. and Patel, V. 2009. Hepatic oxidative stress and apoptosis following acute alcohol. Alcohol & Alcoholism. 44, p. p85.

Assessment of shelf-life of irradiated frozen chicken
Alnasser, M.A., Park, S.F., Jenesson, P. and Tewfik, I. 2009. Assessment of shelf-life of irradiated frozen chicken. International Journal of Food Safety, Nutrition and Public Health. 2 (1), pp. 48-58. https://doi.org/10.1504/IJFSNPH.2009.026918

Would it still be possible to identify irradiated lipid-containing foods towards the end of their shelf-life?
Tewfik, I. and Tewfik, S. 2008. Would it still be possible to identify irradiated lipid-containing foods towards the end of their shelf-life? Food Science and Technology International. 14 (6), pp. 519-524. https://doi.org/10.1177/1082013208100465

Extraction and identification of cyclobutanones from irradiated cheese employing a rapid direct solvent extraction method
Tewfik, I. 2008. Extraction and identification of cyclobutanones from irradiated cheese employing a rapid direct solvent extraction method. International Journal of Food Sciences and Nutrition. 59 (7 & 8), pp. 590-598. https://doi.org/10.1080/09637480701515450

Inter-laboratory trial to validate the direct solvent extraction method for the identification of 2-dodecylcyclobutanone in irradiated chicken and whole liquid egg
Tewfik, I. 2008. Inter-laboratory trial to validate the direct solvent extraction method for the identification of 2-dodecylcyclobutanone in irradiated chicken and whole liquid egg. Food Science and Technology International. 14 (3), pp. 277-283. https://doi.org/10.1177/1082013208095689

Does prolonged breastfeeding reduce the risk for childhood leukemia and lymphomas?
Bener, A., Hoffmann, G.F., Afify, Z., Rasul, K., Mian, M., Guiter, G. and Tewfik, I. 2008. Does prolonged breastfeeding reduce the risk for childhood leukemia and lymphomas? Minerva Pediatrica. 60 (2), pp. 155-161.

Research Student Supervision: Developing Reflective Practice in Higher Education
Tewfik, I. 2008. Research Student Supervision: Developing Reflective Practice in Higher Education. in: World Sustainable Development Outlook 2008: Managing Science and Technology for a Sustainable Future Routledge. pp. 85-92

FISH glossary: an overview of the fluorescence in situ hybridization technique.
Volpi, E.V. and Bridger, J.M. 2008. FISH glossary: an overview of the fluorescence in situ hybridization technique. BioTechniques. 45 (4), pp. 385-409. https://doi.org/10.2144/000112811

Nutraceuticals, functional foods and botanical dietary supplements; promote wellbeing and underpin public health
Tewfik, S. and Tewfik, I. 2008. Nutraceuticals, functional foods and botanical dietary supplements; promote wellbeing and underpin public health. World Review of Science, Technology and Sustainable Development. 5 (2), pp. 104-123. https://doi.org/10.1504/WRSTSD.2008.018552

Childhood obesity prevention (CHOP) programme: a conceptual framework for nutrition intervention
Tewfik, I. 2008. Childhood obesity prevention (CHOP) programme: a conceptual framework for nutrition intervention. International Journal of Food Safety, Nutrition and Public Health. 1 (1), pp. 16-32. https://doi.org/10.1504/IJFSNPH.2008.018853

Metabolic Syndrome and its components among Qatari population
Musallam, M., Bener, A., Zirie, M., Al-Gaud, Y.K., Al-Hamaq, A.A., Othman, M.A. and Tewfik, I. 2008. Metabolic Syndrome and its components among Qatari population. International Journal of Food Safety, Nutrition and Public Health. 1 (1), pp. 88-102. https://doi.org/10.1504/IJFSNPH.2008.018858

Impact of lifestyle and dietary habits on hypovitaminosis D in type 1 diabetes mellitus and healthy children from Qatar, a sun-rich country
Bener, A., Alsaied, A., Al-Ali, M., Hassan, A.S., Basha, B., Al-Kubaisi, A., Abraham, A., Mian, M., Guiter, G., Tewfik, I. and Agha, M. 2008. Impact of lifestyle and dietary habits on hypovitaminosis D in type 1 diabetes mellitus and healthy children from Qatar, a sun-rich country. Annals of Nutrition and Metabolism. 53 (3-4), pp. 215-222. https://doi.org/10.1159/000184439

A rapid direct solvent extraction (DSE) method for the extraction of cyclobutanones from irradiated chicken and liquid whole egg
Tewfik, I. 2008. A rapid direct solvent extraction (DSE) method for the extraction of cyclobutanones from irradiated chicken and liquid whole egg. International Journal of Food Science and Technology. 43 (1), pp. 108-113. https://doi.org/10.1111/j.1365-2621.2006.01399.x

Nutrition and alcoholic liver disease
Everitt, H.E., Patel, V. and Tewfik, I. 2007. Nutrition and alcoholic liver disease. Nutrition Bulletin. 32 (2), pp. 138-144. https://doi.org/10.1111/j.1467-3010.2007.00627.x

Nutrition and alcoholic liver disease
Everitt, H, Patel, V.B and Tewfik, I. 2007. Nutrition and alcoholic liver disease. Nutrition Bulletin. 32 (2), pp. 138 - 144. https://doi.org/10.1111/j.1467-3010.2007.00627.xopen_in_newISSN

Research Supervision: Training, process and experience
Tewfik, I. 2007. Research Supervision: Training, process and experience. in: World Sustainable Development Outlook 2007: Knowledge Management and Sustainable Development in the 21st Century Routledge.

Plasma 5-HT And Fatigue During Endurance Cycle Exercise: Effects Of Exercise Intensity And Duration
Vleck, Veronica E, Asensio, Karine, Tewfik, Ihab, Webb, Heather, McNaughton, Lars, Lowry, Christopher and Bentley, David J 2007. Plasma 5-HT And Fatigue During Endurance Cycle Exercise: Effects Of Exercise Intensity And Duration. Medicine & Science in Sports & Exercise. 39 (5). https://doi.org/10.1249/01.mss.0000274050.00286.a9

Continuity of care 2006: what have we learned since 2000 and what are policy imperatives now?
Freeman, G., Woloshynowych, M., Baker, R., Boulton, M., Guthrie, B., Car, J., Haggerty, J. and Tarrant, C. 2007. Continuity of care 2006: what have we learned since 2000 and what are policy imperatives now? NCCSDO.

Endothelial Nox2 overexpression potentiates vascular oxidative stress and hemodynamic response to angiotensin II: studies in endothelial-targeted Nox2 transgenic mice.
Bendall, J.K., Rinze, R., Adlam, D., Tatham, A.L., de Bono, J., Wilson, N., Volpi, E. and Channon, K.M. 2007. Endothelial Nox2 overexpression potentiates vascular oxidative stress and hemodynamic response to angiotensin II: studies in endothelial-targeted Nox2 transgenic mice. Circulation Research. 100, pp. 1016-1025. https://doi.org/10.1161/01.res.0000263381.83835.7b

Replication profile of PCDH11X and PCDH11Y, a gene pair located in the non-pseudoautosomal homologous region Xq21.3/Yp11.2.
Wilson, N.D., Ross, L.J., Close, J., Mott, R., Crow, T.J. and Volpi, E.V. 2007. Replication profile of PCDH11X and PCDH11Y, a gene pair located in the non-pseudoautosomal homologous region Xq21.3/Yp11.2. Chromosome Research. 15, pp. 485-498. https://doi.org/10.1007/s10577-007-1153-y

Search for neuroblastoma loci: characterization of tumor cell lines that could facilitate their positional cloning.
Romani, M., Muresu, R., Volpi, E.V., Rozzo, C., Marchi, J.M. and Casciano, I. 2007. Search for neuroblastoma loci: characterization of tumor cell lines that could facilitate their positional cloning. Journal of Neuro-oncology . 31, pp. 41-47. https://doi.org/10.1023/a:1005725112688

Communication Patterns in a UK Emergency Department
Woloshynowych, M., Davis, R., Brown, R. and Vincent, C. 2007. Communication Patterns in a UK Emergency Department. Annals of Emergency Medicine. 50 (4), pp. 407-413. https://doi.org/10.1016/j.annemergmed.2007.08.001

Patient safety and iatrogenesis
Woloshynowych, M. and Vincent, C. 2007. Patient safety and iatrogenesis. in: Ayers, S., Baum, A., McManus, C., Newman, S, Wallston, K., Weinman, J. and West, R. (ed.) Cambridge Handbook of Psychology, Health and Medicine. 2nd edition Cambridge University Press.

The public health advocate: food irradiation's offer to food security
Tewfik, I. and Tewfik, S. 2007. The public health advocate: food irradiation's offer to food security. in: Ahmed, A. (ed.) Science, technology and sustainability in the Middle East and North Africa Genève InderScience. pp. 197-209

Competitive food safety program: the case of Qatar
Al-Hamaq, A.A., Zeyadah, S.S., Ahmed, A., Amuna, P. and Tewfik, I. 2007. Competitive food safety program: the case of Qatar. in: Ahmed, A. (ed.) Science, technology and sustainability in the Middle East and North Africa Genève InderScience. pp. 210-228

Book review: B. Freire Wilma (editor). Nutrition and an Active Life: From Knowledge to Action. Scientific and Technical Publication No. 612. Washington DC: Pan American Health Organization 2005. US$ 36·00 (paperback) pp. 260. ISBN: 92 75 11612 1
Tewfik, I. 2006. Book review: B. Freire Wilma (editor). Nutrition and an Active Life: From Knowledge to Action. Scientific and Technical Publication No. 612. Washington DC: Pan American Health Organization 2005. US$ 36·00 (paperback) pp. 260. ISBN: 92 75 11612 1. British Journal of Nutrition. 96 (4), p. 793. https://doi.org/10.1079/BJN20061844

Prevalence of overweight, obesity, and associated psychological problems in Qatari's female population
Bener, A. and Tewfik, I. 2006. Prevalence of overweight, obesity, and associated psychological problems in Qatari's female population. Obesity Reviews. 7 (2), pp. 139-145. https://doi.org/10.1111/j.1467-789X.2006.00209.x

Prevalence of overweight, obesity, and associated psychological problems in Qatari's female population
Bener, A. and Tewfik, I. 2006. Prevalence of overweight, obesity, and associated psychological problems in Qatari's female population. Obesity Reviews. 7 (2), pp. 139-145. https://doi.org/10.1111/j.1467-789X.2006.00209.x

Incident reporting in one UK accident and emergency department
Tighe, C.M., Woloshynowych, M., Brown, R., Wears, B. and Vincent, C. 2006. Incident reporting in one UK accident and emergency department. Accident and Emergency Nursing. 14 (1), pp. 27-37. https://doi.org/10.1016/j.aaen.2005.10.001

PCDH11 is X/Y homologous in Homo sapiens but not in Gorilla gorilla and Pan troglodytes.
Wilson, N.D., Ross, L.J., Crow, T.J. and Volpi, E.V. 2006. PCDH11 is X/Y homologous in Homo sapiens but not in Gorilla gorilla and Pan troglodytes. Cytogenetic and Genome Research. 114, pp. 137-139. https://doi.org/10.1159/000093329

The leukocyte receptor complex in chicken is characterized by massive expansion and diversification of immunoglobulin-like Loci.
Laun, K., Coggill, P., Palmer, S., Sims, S., Ning, Z., Ragoussis, J., Volpi, E., Wilson, N., Beck, S., Ziegler, A. and Volz, A. 2006. The leukocyte receptor complex in chicken is characterized by massive expansion and diversification of immunoglobulin-like Loci. PLoS Genetics. 2 (5) e73. https://doi.org/10.1371/journal.pgen.0020073

The effects of stress on surgical performance.
Wetzel, C. M., Kneebone, R., Woloshynowych, M., Nestel, D., Moorthy, K., Kidd, J. and Darzi, A. 2006. The effects of stress on surgical performance. The American Journal of Surgery. 191 (1), pp. 5-10. https://doi.org/10.1016/j.amjsurg.2005.08.034

Techniques used in the investigation and analysis of critical incidents in healthcare
Rogers, S, Taylor-Adams, S. and Woloshynowych, M. 2006. Techniques used in the investigation and analysis of critical incidents in healthcare. in: Walshe, K. and Boaden, R. (ed.) Patient Safety: Research into Practice Maidenhead, UK Open University Press. pp. 130-143

Industrial and dietetic applications of the food multimix (FMM) concept in meeting the nutritional needs of vulnerable groups in South Africa
Zotor, F., Amuna, P., Oldewage-Theron, W.H., Adewuya, T., Prinsloo, G., Chinyanga, Y., Tewfik, I. and Amuna, N. 2006. Industrial and dietetic applications of the food multimix (FMM) concept in meeting the nutritional needs of vulnerable groups in South Africa. Academic Journal of Vaal University of Technology. 3, pp. 54-67.

CO2 photoassimilation, chlorophyll fluorescence, lipid peroxidation and yield in cotton (Gossypium hirsutum L. cv Giza 65) in response to O3
Hassan, I.A. and Tewfik, I. 2006. CO2 photoassimilation, chlorophyll fluorescence, lipid peroxidation and yield in cotton (Gossypium hirsutum L. cv Giza 65) in response to O3. World Review of Science, Technology and Sustainable Development. 3 (1), pp. 70-79. https://doi.org/10.1504/WRSTSD.2006.008764

Prevalence of dieting, overweight, body image satisfaction and associated psychological problems in adolescent boys
Bener, A., Kamal, A., Tewfik, I. and Sabuncuoglu, O. 2006. Prevalence of dieting, overweight, body image satisfaction and associated psychological problems in adolescent boys. Nutrition & Food Science. 36 (5), pp. 295-304. https://doi.org/10.1108/00346650610703144

Health habits and risk of obesity among 6–13 year old school children and adolescents
Al-Shammari, H., Bumejjdad, A., Amuna, P. and Tewfik, I. 2005. Health habits and risk of obesity among 6–13 year old school children and adolescents. in: Nutrition and health - Current Topics IV Garant. pp. 207–208

An interstitial deletion-insertion involving chromosomes 2p25.3 and Xq27.1, near SOX3, causes X-linked recessive hypoparathyroidism.
Bowl, M.R., Nesbit, M.A., Harding, B., Levy, E., Jefferson, A., Volpi, E.V., Rizzoti, K., Lovell-Badge, R., Schlessinger, D., Whyte, M.P. and Thakker, R.V. 2005. An interstitial deletion-insertion involving chromosomes 2p25.3 and Xq27.1, near SOX3, causes X-linked recessive hypoparathyroidism. Journal of Clinical Investigation. 115 (10), pp. 2822-2831. https://doi.org/10.1172/jci24156

The investigation and analysis of critical incidents and adverse events in healthcare
Woloshynowych, M., Rogers, S., Taylor-Adams, S. and Vincent, C. 2005. The investigation and analysis of critical incidents and adverse events in healthcare. Health Technology Assessment. 9 (19). https://doi.org/10.3310/hta9190

Error Reduction in Medicine: Final report to the Nuffield Trust
Lyons, M., Woloshynowych, M., Adams, S. and Vincent, C. 2004. Error Reduction in Medicine: Final report to the Nuffield Trust. Nuffield Trust.

Unexpected complexity in the haplotypes of commonly used inbred strains of laboratory mice
Yalcin, B., Fullerton, J., Miller, S., Keays, D.A., Brady, S., Bhomra, A., Jefferson, A., Volpi, E., Copley, R.R., Flint, J. and Mott, R. 2004. Unexpected complexity in the haplotypes of commonly used inbred strains of laboratory mice. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.0401189101

Mapping of three translocation breakpoints associated with orofacial clefting within 6p24 and identification of new transcripts within the region
Davies, S.J., Wise, C., Venkatesh, B., Mirza, G., Jefferson, A., Volpi, E.V. and Ragoussis, J. 2004. Mapping of three translocation breakpoints associated with orofacial clefting within 6p24 and identification of new transcripts within the region. Cytogenetic and Genome Research. 105, pp. 47-53. https://doi.org/10.1159/000078008

Would food irradiation as a means of technology transfer assist food productivity and security in Africa and sustain its development?
Tewfik, I., Amuna, P. and Zotor, F. 2004. Would food irradiation as a means of technology transfer assist food productivity and security in Africa and sustain its development? International Journal of Technology Policy and Management. 4 (1), pp. 44-52.

Human and economic development in developing countries: a public health dimension employing the food multimix concept
Amuna, P., Zotor, F. and Tewfik, I. 2004. Human and economic development in developing countries: a public health dimension employing the food multimix concept. World Review of Science, Technology and Sustainable Development. 1 (2), pp. 129-137.

Retrospective case record review: a blunt instrument that needs sharpening
Neale, G. and Woloshynowych, M. 2003. Retrospective case record review: a blunt instrument that needs sharpening. BMJ Quality and Safety. 12 (1), pp. 2-3. https://doi.org/10.1136/qhc.12.1.2

Cohesion, but not too close
Volpi, E.V., Sheer, D. and Uhlmann, F. 2001. Cohesion, but not too close. Current Biology. 11 (10), p. R378. https://doi.org/10.1016/s0960-9822(01)00212-3

Exploring the causes of adverse events in British NHS hospital practice.
Neale, G., Woloshynowych, M. and Vincent, C. 2001. Exploring the causes of adverse events in British NHS hospital practice. Journal of the Royal Society of Medicine. 94, pp. 322-220. https://doi.org/10.1177/014107680109400702

Adverse events in British hospitals: preliminary retrospective record review
Vincent, C., Neale, G. and Woloshynowych, M. 2001. Adverse events in British hospitals: preliminary retrospective record review. BMJ. 322, pp. 517-519. https://doi.org/10.1136/bmj.322.7285.517

Large-scale chromatin organization of the major histocompatibility complex and other regions of human chromosome 6 and its response to interferon in interphase nuclei.
Volpi, E.V., Chevret, E., Jones, T., Vatcheva, R., Williamson, J., Beck, S., Campbell, R.D., Goldsworthy, M., Powis, S.H., Ragoussis, J., Trowsdale, J. and Sheer, D. 2000. Large-scale chromatin organization of the major histocompatibility complex and other regions of human chromosome 6 and its response to interferon in interphase nuclei. Journal of Cell Science. 113 (Pt 9), pp. 1565-1576. https://doi.org/10.1242/jcs.113.9.1565

Mini review: form and function in the human interphase chromosome
Chevret, E., Volpi, E.V. and Sheer, D. 2000. Mini review: form and function in the human interphase chromosome. Cytogenetics and Cell Genetics. 90 (1-2), pp. 13-21. https://doi.org/10.1159/000015654

The effect of intermittent heating on some chemical parameters of refined oils used in Egypt. A public health nutrition concern
Tewfik, I., Ismail, H. and Sumar, S. 1998. The effect of intermittent heating on some chemical parameters of refined oils used in Egypt. A public health nutrition concern. International Journal of Food Sciences and Nutrition. 49 (5), pp. 339-342. https://doi.org/10.3109/09637489809089408

Nucleotide sequence and chromosomal mapping of the 5S rDNA repeat of the crustacean Proasellus coxalis.
Pelliccia, F., Barzotti, R., Volpi, E.V., Bucciarelli, E. and Rocchi, A. 1998. Nucleotide sequence and chromosomal mapping of the 5S rDNA repeat of the crustacean Proasellus coxalis. Genome. 41 (1), pp. 129-123. https://doi.org/10.1139/g97-110

Numerical abnormalities of chromosomes 1 and 10 in endometrial adenocarcinoma: fluorescence in situ hybridization analysis of 23 archival paraffin-embedded samples.
Muresu, R., Cossu, A., Scarpa, A.M., Volpi, E.V., Rocca, P.C., Pintus, A., Tibiletti, M.G., Tanda, F., Pirastu, M. and Massarelli, G. 1998. Numerical abnormalities of chromosomes 1 and 10 in endometrial adenocarcinoma: fluorescence in situ hybridization analysis of 23 archival paraffin-embedded samples. Cancer Genetics and Cytogenetics. 107 (1), pp. 37-42. https://doi.org/10.1016/s0165-4608(98)00059-4

A rapid supercritical fluid extraction method for the detection of 2-alkylcyclobutanones in gamma irradiated beef and chicken
Tewfik, I., Ismail, H. and Sumar, S. 1998. A rapid supercritical fluid extraction method for the detection of 2-alkylcyclobutanones in gamma irradiated beef and chicken. Lebensmittel-Wissenschaft und-Technologie = Journal of Food Sciences and Technology. 31 (4), pp. 366-370. https://doi.org/10.1006/fstl.1998.0371

A rapid direct solvent extraction method (DSE-GC-MS) used to detect irradiated minced beef
Tewfik, I., Ismail, H. and Sumar, S. 1998. A rapid direct solvent extraction method (DSE-GC-MS) used to detect irradiated minced beef. Bulletin of High Institute of Public Health. 28 (3), pp. 85-92.

A rapid supercritical fluid extraction method for the qualitative detection of 2-alkylcyclobutanones in gamma-irradiated fresh and sea water fish
Tewfik, I. 1998. A rapid supercritical fluid extraction method for the qualitative detection of 2-alkylcyclobutanones in gamma-irradiated fresh and sea water fish. International Journal of Food Sciences and Nutrition. 50 (1), pp. 51-56. https://doi.org/10.1080/096374899101418

Immediate Recovery of Psychomotor Function After Patient-Administered Nitrous Oxide/Oxygen Inhalation for Colonoscopy
Trojan, J., Saunders, B.P., Woloshynowych, M., Debinsky, H.S. and Williams, C.B. 1997. Immediate Recovery of Psychomotor Function After Patient-Administered Nitrous Oxide/Oxygen Inhalation for Colonoscopy. Endoscopy. 29 (1), pp. 17-22. https://doi.org/10.1055/s-2007-1004055

An integrated physical and genetic map spanning chromosome band 10q24.
Gray, I.C., Fallowfield, J., Ford, S., Nobile, C., Volpi, E.V. and Spurr, N.K. 1997. An integrated physical and genetic map spanning chromosome band 10q24. Genomics. 43 (1), pp. 85-88. https://doi.org/10.1006/geno.1997.4809

Detailed physical analysis of a 1.5-megabase YAC contig containing the MXI1 and ADRA2A genes.
Manca, A., Volpi, E.V., Laficara, F., Muresu, R., Gray, I.C., Spurr, N.K. and Nobile, C. 1997. Detailed physical analysis of a 1.5-megabase YAC contig containing the MXI1 and ADRA2A genes. Genomics. 45 (2), pp. 407-411. https://doi.org/10.1006/geno.1997.4924

The Patient Requests Form: a way of measuring what patients want from their general practitioner.
Valori, R., Woloshynowych, M., Bellenger, N., Aluvihare, V. and Salmon, P. 1996. The Patient Requests Form: a way of measuring what patients want from their general practitioner. Journal of Psychosomatic Research. 40 (1), pp. 87-94. https://doi.org/10.1016/0022-3999(95)00548-X

The measurement of beliefs about physical symptoms in English general practice patients
Salmon, P., Woloshynowych, M. and Valori, R. 1996. The measurement of beliefs about physical symptoms in English general practice patients. Social Science & Medicine. 42 (11), pp. 1561-1567. https://doi.org/10.1016/0277-9536(95)00263-4

Psychological aspects of gastrointestinal endoscopy: a review
Woloshynowych, M., Oakley, D.A., Saunders, B.P. and Williams, C.B. 1996. Psychological aspects of gastrointestinal endoscopy: a review. Endoscopy. 28 (9), pp. 763-767. https://doi.org/10.1055/s-2007-1005602

Taxonomic and biogeographic analysis of the Proasellus coxalis-group (Crustacea, Isopoda, Asellidae) in Sicily, with description of Proasellus montalentii n.sp.
Stoch, F., Valentino, F. and Volpi, E. 1996. Taxonomic and biogeographic analysis of the Proasellus coxalis-group (Crustacea, Isopoda, Asellidae) in Sicily, with description of Proasellus montalentii n.sp. Hydrobiologia. 317, pp. 247-258. https://doi.org/10.1007/bf00036474

More detailed characterization of some of the HL60 karyotypic features by fluorescence in situ hybridization.
Volpi, E.V., Vatcheva, R., Labella, T. and Gan, S.U. 1996. More detailed characterization of some of the HL60 karyotypic features by fluorescence in situ hybridization. Cancer Genetics and Cytogenetics. 87 (2), pp. 103-106. https://doi.org/10.1016/0165-4608(95)00214-6

Molecular and genetic studies on the region of translocation and duplication in the neuroblastoma cell line NGP at the 1p36.13-p36.32 chromosomal site
Casciano, I., Marchi, J.V.M., Muresu, R., Volpi, E.V., Rozzo, C., Opdenakker, G. and Romani, M. 1996. Molecular and genetic studies on the region of translocation and duplication in the neuroblastoma cell line NGP at the 1p36.13-p36.32 chromosomal site. Oncogene. 12 (10), pp. 2101-2108.

A rapid method (SFE-GC-MS) used to detect irradiated minced beef
Tewfik, I., Ismail, H., Amine, E. and Rady, A. 1996. A rapid method (SFE-GC-MS) used to detect irradiated minced beef. Arab Journal of Nuclear Sciences and Applications. 29 (4), pp. 345-353.

Simple DNA repeats and sex chromosome differentiation in asellus aquaticus (Crustacea, isopoda)
Volpi, E.V., Pelliccia, F., Lanza, V., Barzotti, R. and Rocchi, A. 1995. Simple DNA repeats and sex chromosome differentiation in asellus aquaticus (Crustacea, isopoda). Heredity. 75, p. 267–272. https://doi.org/10.1038/hdy.1995.134

Cytogenetic and molecular studies on the neuroblastoma cell line NGP: identification of a reciprocal t(1;15) involving the "consensus region" 1p36.1.
Muresu, R., Casciano I., Volpi, E.V., Siniscalco, M. and Romani, M. 1995. Cytogenetic and molecular studies on the neuroblastoma cell line NGP: identification of a reciprocal t(1;15) involving the "consensus region" 1p36.1. Genes, Chromosomes & Cancer. 13 (1), pp. 66-71. https://doi.org/10.1002/gcc.2870130111

Subregional mapping of the human lymphocyte-specific protein tyrosine kinase gene (LCK) to 1p35-->p34.3 and its position relative to the 1p marker D1S57.
Volpi, E.V., Romani, M. and Siniscalco, M. 1994. Subregional mapping of the human lymphocyte-specific protein tyrosine kinase gene (LCK) to 1p35-->p34.3 and its position relative to the 1p marker D1S57. Cytogenetics and Cell Genetics. 67, pp. 187-189. https://doi.org/10.1159/000133819

Telomeric sequences of ase/lus aquaticus (Crust. isop.)
Pelliccia, F., Volpi, E.V., Lanza, V., Gaddini, L., Balding, A. and Rocchi, A. 1994. Telomeric sequences of ase/lus aquaticus (Crust. isop.). Heredity. 72, pp. 78-80. https://doi.org/10.1038/hdy.1994.9

Concurrent mapping of an adenovirus 5/SV40 integration site and the U1 snRNA cluster (RNU1) within 400 kb of the chromosome region 1p36.1.
Romani, M., Baldini, A., Volpi, E., Casciano, I., Nobile, C., Muresu, R. and Siniscalco, M. 1994. Concurrent mapping of an adenovirus 5/SV40 integration site and the U1 snRNA cluster (RNU1) within 400 kb of the chromosome region 1p36.1. Cytogenetics and Cell Genetics. 67, pp. 37-40. https://doi.org/10.1159/000133793

MULTIPRINS: a method for multicolour primed in situ labelling
Volpi, E. and Baldini, A. 1993. MULTIPRINS: a method for multicolour primed in situ labelling. Chromosome Research. 1, pp. 257-260. https://doi.org/10.1007/bf00710131

Morphological differentiation of a sex chromosome and ribosomal genes in asellus aquaticus (Crust. Isop.)
Volpi, E.V., Pelliccia, F., Lanza, V., Di Castro, M. and Rocchi, A. 1992. Morphological differentiation of a sex chromosome and ribosomal genes in asellus aquaticus (Crust. Isop.). Heredity. https://doi.org/10.1038/hdy.1992.153

GATA repeats in the genome of Asellus aquaticus (Crustacea, Isopoda).
Pelliccia, F., Di Castro, M., Lanza, V., Volpi, E.V. and Rocchi, A. 1991. GATA repeats in the genome of Asellus aquaticus (Crustacea, Isopoda). Chromosoma. 100, pp. 152-155. https://doi.org/10.1007/bf00337243

Light-dark cycle and mitotic index in Asellus aquaticus (L.) (Crustacea, Isopoda)
Valentino, F., Volpi, E.V., Antolini, R. and Migliore, L. 1990. Light-dark cycle and mitotic index in Asellus aquaticus (L.) (Crustacea, Isopoda). Genetica. 82, pp. 147-150. https://doi.org/10.1007/bf00124645

The genetic isolation between some populations of Proasellus coxalis
Volpi, E.V., Antolini, R. and Valentino, F. 1989. The genetic isolation between some populations of Proasellus coxalis. Journal of Zoological Systematics and Evolutionary Research. 27 (3), pp. 246-251. https://doi.org/10.1111/j.1439-0469.1989.tb00346.x

Characterization of the karyotype of proasellus meridianusby differential staining techniques
Di Castro, M., Lanza, V., Volpi, E.V. and Rocchi, A. 1989. Characterization of the karyotype of proasellus meridianusby differential staining techniques. Caryologia. 42 (3-4), pp. 235-242. https://doi.org/10.1080/00087114.1989.10796970

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