Title | Molecularly imprinted polymer based on MWCNTs-QDs as fluorescent biomimetic sensor for specific recognition of target protein |
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Authors | Ding, X., Tao, L., Quan, J., Nie, H., Gong, X., Bligh, S.W.A. and Limin, Z. |
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Abstract | A novel molecularly imprinted optosensing material based on multi-walled carbon nanotube-quantum dots (MWCNT-QDs) has been designed and synthesized for its high selectivity, sensitivity and specificity in the recognition of a target protein bovine serum albumin (BSA). Molecularly imprinted polymer coated MWCNT-QDs using BSA as the template (BMIP-coated MWCNT-QDs) exhibits a fast mass-transfer speed with a response time of 25 min. It is found that the BSA as a target protein can significantly quench the luminescence of BMIP-coated MWCNT-QDs in a concentration-dependent manner that is best described by a Stem-Volmer equation. The K-SV for BSA is much higher than bovine hemoglobin and lysozyme, implying a highly selective recognition of the BMIP-coated MWCNT-QDs to BSA. Under optimal conditions, the relative fluorescence intensity of BMIP-coated MWCNT-QDs decreases linearly with the increasing target protein BSA in the concentration range of 5.0 x 10(-7)-35.0 x 10(-7) M with a detection limit of 80 nM. |
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Keywords | protein recognition |
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| fluorescent biomimetic sensor |
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| molecularly imprinted polymer |
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| multi-walled carbon nanotubes |
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| quantum dots |
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Journal | Materials Science and Engineering C |
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Journal citation | 48, pp. 469-479 |
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ISSN | 0928-4931 |
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Year | 2015 |
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Accepted author manuscript | |
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Digital Object Identifier (DOI) | https://doi.org/10.1016/j.msec.2014.12.032 |
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Web address (URL) | http://dx.doi.org/10.1016/j.msec.2014.12.032 |
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Publication dates |
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Published | 01 Mar 2015 |
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Funder | .No external funding |
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