Title | Hydrophobically modified chitosan nanoliposomes for intestinal drug delivery |
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Type | Journal article |
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Authors | Zariwala, M., Bendre, H., Markiv, A., Farnaud, S., Renshaw, D., Taylor, K.M.G. and Somavarapu, S. |
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Abstract | Background: Encapsulation of hydrophilic drugs within liposomes can be challenging. Methods: A novel chitosan derivative, O-palmitoyl chitosan (OPC) was synthesized from chitosan and palmitoyl chloride using methane-sulfonic acid as a solvent. The success of synthesis was confirmed by Fourier transform infra-red (FT-IR) spectroscopy and proton NMR spectroscopy (H-NMR). Liposomes encapsulating ferrous sulphate as a model hydrophilic drug for intestinal delivery were prepared with or without OPC inclusion (Lipo-Fe and OPC-Lipo-Fe). Results: Entrapment of iron was significantly higher in OPC containing liposomes compared to controls. Quantitative iron absorption from the OPC liposomes was significantly higher (1.5-fold P<0.05) than free ferrous sulphate controls. Qualitative uptake analysis by confocal imaging using coumarin-6 dye loaded liposomes also indicated higher cellular uptake and internalization of the OPC-containing liposomes. Conclusion: These findings suggest that addition of OPC during liposome preparation creates robust vesicles that have improved mucoadhesive and absorption enhancing properties. The chitosan derivative OPC therefore provides a novel alternative for formulation of delivery vehicles targeting intestinal absorption. |
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Keywords | gut delivery, intestinal absorption, Caco-2, ferrous sulfate |
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Journal | International Journal of Nanomedicine |
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Journal citation | 2018:13, pp. 5837-5848 |
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ISSN | 1176-9114 |
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Year | 2018 |
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Publisher | Dove Medical Press Ltd. |
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Publisher's version | |
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Digital Object Identifier (DOI) | https://doi.org/10.2147/IJN.S166901 |
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Publication dates |
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Published | 27 Sep 2018 |
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License | CC BY-NC 3.0 |
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