Enhanced production of biobased, biodegradable, Poly(3-hydroxybutyrate) using an unexplored marine bacterium Pseudohalocynthiibacter aestuariivivens, isolated from highly polluted coastal environment.

Esposito, Fortunato Palma, Vecchiato, Vittoria, Buonocore, Carmine, Tedesco, Pietro, Noble, Brendon, Basnett, Pooja and de Pascale, Donatella 2023. Enhanced production of biobased, biodegradable, Poly(3-hydroxybutyrate) using an unexplored marine bacterium Pseudohalocynthiibacter aestuariivivens, isolated from highly polluted coastal environment. Bioresource Technology. 368, p. 128287. https://doi.org/10.1016/j.biortech.2022.128287

TitleEnhanced production of biobased, biodegradable, Poly(3-hydroxybutyrate) using an unexplored marine bacterium Pseudohalocynthiibacter aestuariivivens, isolated from highly polluted coastal environment.
TypeJournal article
AuthorsEsposito, Fortunato Palma, Vecchiato, Vittoria, Buonocore, Carmine, Tedesco, Pietro, Noble, Brendon, Basnett, Pooja and de Pascale, Donatella
AbstractThe production and disposal of plastics from limited fossil reserves, has prompted research for greener and sustainable alternatives. Polyhydroxyalkanoates (PHAs) are biocompatible, biodegradable, and thermoprocessable polyester produced by microbes. PHAs found several applications but their use is limited due to high production cost and low yields. Herein, for the first time, the isolation and characterization of Pseudohalocynthiibacter aestuariivivens P96, a marine bacterium able to produce surprising amount of PHAs is reported. In the best growth condition P96 was able to reach a maximum production of 4.73 g/L, corresponding to the 87 % of total cell dry-weight. Using scanning and transmission microscopy, lab-scale fermentation, spectroscopic techniques, and genome analysis, the production of thermoprocessable polymer Polyhydroxybutyrate P(3HB), a PHAs class, endowed with mechanical and thermal properties comparable to that of petroleum-based plastics was confirmed. This study represents a milestone toward the use of this unexplored marine bacterium for P(3HB) production. [Abstract copyright: Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.]
KeywordsBlue biotechnology
Marine bacteria
Thermoplasticity
Polyhydroxyalkanoate
JournalBioresource Technology
Journal citation368, p. 128287
ISSN1873-2976
Year2023
PublisherElsevier
Publisher's version
License
CC BY-NC-ND 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1016/j.biortech.2022.128287
PubMed ID36368485
Publication dates
Published online09 Nov 2022
Published in print2023

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