|Title||Manganese enhancement in non-CNS organs|
|Authors||Lee, L.W., So, P.W., Price, A.N., Parkinson, J.R.C., Larkman, D.J., Halliday, J., Poucher, S.M., Pugh, J.A.T., Cox, A.G., McLeod, C.W. and Bell, J.D.|
Manganese-enhanced magnetic resonance imaging (MEMRI) is a novel imaging technique capable of monitoring calcium influx, in vivo. Manganese (Mn2+) ions, similar to calcium ions (Ca2+), are taken up by activated cells where their paramagnetic properties afford signal enhancement in T1-weighted MRI methodologies. In this study we have assessed Mn2+ distribution in mice using magnetization-prepared rapid gradient echo (MP-RAGE) based MRI, by measuring changes in T1–effective relaxation times (T1-eff), effective R1-relaxation rates (R1-eff) and signal intensity (SI) profiles over time. The manganese concentration in the tissue was also determined using inductively coupled plasma atomic emission spectrometry (ICP-AES). Our results show a strong positive correlation between infused dose of MnCl2 and the tissue manganese concentration. Furthermore, we demonstrate a linear relationship between R1-eff and tissue manganese concentration and tissue-specific Mn2+ distribution in murine tissues following dose-dependent Mn2+ administration. This data provides an optimized MnCl2 dose regimen for an MP-RAGE based sequence protocol for specific target organs and presents a potential 3D MRI technique for in vivo imaging of Ca2+ entry during Ca2+-dependent processes in a wide range of tissues.
|Journal||NMR in Biomedicine|
|Journal citation||23 (8), pp. 931-938|
|Digital Object Identifier (DOI)||https://doi.org/10.1002/nbm.1513|