| Title | Non-transferrin-bound iron in plasma or serum from patients with idiopathic hemochromatosis. Characterization by high performance liquid chromatography and nuclear magnetic resonance spectroscopy |
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| Authors | Grootveld, M., Bell, J.D., Halliwell, B., Aruoma, O.I., Bomford, A. and Sadler, P.J. |
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| Abstract | The nature of non-transferrin-bound iron in the plasma or serum of iron-overloaded hemochromatosis patients was studied by high performance liquid chromatography (HPLC) and high resolution nuclear magnetic resonance (NMR). 500-MHz proton Hahn spin-echo NMR spectra of plasma or serum, combined with the use of the iron chelator desferrioxamine, suggests complexation of iron ions with citrate and a possible involvement of acetate. Addition of FeCl3 to hemochromatosis samples broadened the NMR signals from citrate. HPLC analysis rigorously confirmed the presence of an iron-citrate complex in ultrafiltrates of plasma or serum studies with added FeCl3 or desferrioxamine supported this conclusion. It is proposed that non-transferrin-bound iron in the plasma of iron-overloaded patients exists largely as complexes with citrate and possibly also as ternary iron-citrate-acetate complexes. The presence of such complexes would account for the ability of non-transferrin-bound iron to be measurable by the bleomycin assay and for its rapid clearance from the circulation by the liver. |
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| Journal | Journal of Biological Chemistry |
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| Journal citation | 264 (8), pp. 4417-4422 |
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| ISSN | 0021-9258 |
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| Year | 15 Mar 1989 |
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| Publisher | American Society for Biochemistry and Molecular Biology |
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| Web address (URL) | http://www.jbc.org/content/264/8/4417.abstract |
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| Publication dates |
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| Published | 15 Mar 1989 |
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