Background Between 2015 and 2024, 113 Low Traffic Neighbourhoods (LTNs) were implemented across Greater London, with 27 subsequently removed. We investigated their impacts on road traffic injuries inside LTNs and on ‘boundary roads’ immediately surrounding the LTNs. Methods We matched police-recorded injuries from STATS19 data to Ordnance Survey road links that were spatially intersected with LTNs/boundary roads. Conditional fixed-effects Poisson regression models used the number of injuries per road link per quarter of each year (January 2012 to June 2024) to test whether LTN implementation was associated with changes in injury rates. Results LTN implementation was associated with a 35% (95% CI 29% to 40%; p<0.001) decrease in all injuries and a 37% (95% CI 24% to 48%; p<0.001) decrease in people Killed or Seriously Injured (KSI). Injuries decreased across a range of casualty and LTN characteristics. However, there was evidence of a smaller benefit in LTNs implemented in Outer London since 2020. Following the removal of an LTN, injury numbers increased back to pre-intervention levels. On boundary roads, there was no evidence of a change in total injury numbers (estimate −2%, 95% CI −5% to +2%) or KSI injury numbers (estimate 0%, 95% CI −7% to +8%). This reflected decreased numbers of injuries on boundary roads for cyclists and motorcyclists, and no change for pedestrians and other motor vehicle users. Conclusion LTNs in London reduced road traffic injuries among all road users inside the LTN areas, with no evidence of overall impact (and for cyclists and motorcyclists a benefit) on boundary roads. |